Date published: 2025-9-8

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Hint1 抗体 (H-5): sc-365015

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说明书
  • Hint1抗体(H-5)是小鼠单克隆IgG1 (kappa轻链),规格为200 µg/ml
  • 免疫原氨基酸序列8-47位于human物种的Hint 1的N-terminus附近
  • 推荐用于 mouse, rat 和 human 来源的Hint1 WB, IP, IF 和 ELISA检测
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是Hint1 Antibody (H-5) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Hint1 Antibody (H-5) 打包提供(请参阅下面的订购信息)。

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    関連項目

    Hint1(组氨酸三联体核苷酸结合蛋白1),也称为PRKCNH1或PKCI1,是一种由126个氨基酸组成的蛋白质,它定位于细胞质和细胞核,并包含一个HIT(组氨酸三联体)结构域。Hint1广泛表达并以同源二聚体的形式存在,它的功能是水解腺苷5'-单磷酰胺底物,如AMP-N-丙氨酸甲酯、AMP-吗啉代和AMP-α-乙酰赖氨酸甲酯,并可能抑制PKC功能并影响细胞凋亡。Hint1 mRNA的敲低导致p53和Bax的下调,这表明Hint1是一个单倍体不足的肿瘤抑制因子。此外,编码Hint1的基因的启动子超甲基化可能在肝癌的发生中发挥作用。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    Hint1 抗体 (H-5) 参考文献:

    1. Hint1基因敲除导致脑中蛋白激酶C伽马激活受损。  |  Zhang, F., et al. 2015. Brain Res. 1622: 196-203. PMID: 26133792
    2. Hint1基因缺陷增强小鼠脊髓上疼痛敏感性。  |  Liu, F., et al. 2016. Brain Behav. 6: e00496. PMID: 27547499
    3. HINT1在甲基苯丙胺诱导的行为敏化中的作用。  |  Liu, P., et al. 2020. Psychopharmacology (Berl). 237: 2345-2351. PMID: 32430517
    4. Hint1 过度表达抑制细胞周期并诱导人类骨肉瘤细胞凋亡。  |  Duan, DD., et al. 2020. Onco Targets Ther. 13: 8223-8232. PMID: 32884300
    5. 导致轴突运动神经病的HINT1人类突变蛋白与伴侣蛋白发生异常相互作用。  |  Cortés-Montero, E., et al. 2021. Mol Neurobiol. 58: 1834-1845. PMID: 33404983
    6. 老年小鼠缺乏 HINT1 会减少焦虑样和抑郁样行为,并提高认知能力。  |  Zhou, Y., et al. 2022. Exp Gerontol. 159: 111683. PMID: 34995725
    7. USP40在足细胞损伤中脱乙酰化HINT1并稳定p53。  |  Takahashi, S., et al. 2022. Biochem Biophys Res Commun. 614: 198-206. PMID: 35605301
    8. HINT1基因的新突变可导致常染色体隐性遗传的轴索神经病伴神经肌强直,卡马西平可有效治疗该病,文献综述。  |  Xu, L., et al. 2022. Acta Neurol Belg. 122: 1305-1312. PMID: 35767146
    9. 立陶宛的HINT1神经病变:临床、遗传和功能分析。  |  Malcorps, M., et al. 2022. Orphanet J Rare Dis. 17: 374. PMID: 36242072
    10. HINT1可促进神经元凋亡,并引发大鼠出现类似精神分裂症的行为。  |  Kang, Y., et al. 2025. Behav Brain Res. 477: 115297. PMID: 39426527

    订购信息

    产品名称产品编号规格价格数量收藏夹

    Hint1 抗体 (H-5)

    sc-365015
    200 µg/ml
    RMB2377.00

    Hint1 (H-5): m-IgG Fc BP-HRP 套装

    sc-537759
    200 µg Ab; 10 µg BP
    RMB2662.00

    Hint1 (H-5): m-IgGκ BP-HRP 套装

    sc-535129
    200 µg Ab; 40 µg BP
    RMB2662.00

    Hint1 (H-5): m-IgG1 BP-HRP 套装

    sc-545338
    200 µg Ab; 20 µg BP
    RMB2662.00

    What is the recommended fixation method for immunofluorescence staining with Hint1 (H-5): sc-365015 monoclonal antibody?

    Asked by: Cweed
    Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
    Answered by: Technical Support
    Date published: 2017-03-01
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    Rated 5 out of 5 by from Produced excellent immunofluorescence cytoplasmicProduced excellent immunofluorescence cytoplasmic and nuclear staining in formalin-fixed HepG2 cells. -SCBT QC
    Date published: 2015-02-14
    Rated 5 out of 5 by from Nice Western Blot data of Hint1 expressionNice Western Blot data of Hint1 expression in Jurkat and HEK293 whole cell lysates. -SCBT QC
    Date published: 2013-06-08
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    Hint1 抗体 (H-5) is rated 5.0 out of 5 by 2.
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