Date published: 2025-9-6
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HGFα Antibody (H-10) - Western Blotting - Image 353344
HGFα 抗体 (H-10): sc-374422. JAR (A) 和 Hep G2 (B) 全细胞裂解液中 HGFα 表达的 Western 印迹分析.
HGFα Antibody (H-10) - Western Blotting - Image 353344
HGFα 抗体 (H-10): sc-374422. JAR (A) 和 Hep G2 (B) 全细胞裂解液中 HGFα 表达的 Western 印迹分析.
HGFα Antibody (H-10) - Immunohistochemistry - Image 159121
HGFa (H-10): sc-374422. 福尔马林固定, 石蜡包埋的人体直肠组织的免疫过氧化物酶染色显示腺细胞的胞浆染色.
HGFα Antibody (H-10) - Western Blotting - Image 150524
HGFα (H-10): sc-374422. 人重组 HGFα 的 Western 印迹分析.
HGFα 抗体 (H-10): sc-374422. JAR (A) 和 Hep G2 (B) 全细胞裂解液中 HGFα 表达的 Western 印迹分析.

HGFα 抗体 (H-10): sc-374422

4.9(10)
寫評論提問

说明书
  • HGF alpha 抗体 H-10 是小鼠单克隆 IgG1 κ，HGFα抗体, 在12篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的HGFα的N-terminus的氨基酸3-27之间
  • HGF alpha 抗体 (H-10) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 HGFα; 也和以下物种反应，包括： 和 equine, canine and porcine
  • 抗HGF alpha抗体（H-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取HGFα (H-10): sc-374422的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 HGFα 抗体 (H-10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与HGFα 抗体 (H-10) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 HGF 抗体 包括 HGF β

HGFα抗体（H-10）是一种IgG1κ小鼠单克隆HGFα抗体（也称为肝细胞生长因子α抗体或HGFα抗体），可通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人源的HGFα蛋白。HGFα抗体（H-10）可以是非偶联的抗HGFα抗体形式，也可以是多种偶联形式的抗HGFα抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。肝细胞生长因子或HGF是一种多效生长因子，也被称为散射因子、造血素A和乳腺生长因子。HGF以728个氨基酸的前体形式合成，具有29个氨基酸的信号肽，该信号肽不存在于成熟蛋白质中。生物活性HGF由二硫键连接的α链和β链组成，二者均高度糖基化。HGF通过HGF受体c-Met发挥其生物学作用，c-Met由正常肝细胞、胃和肠上皮细胞、卵巢和子宫内膜内皮细胞以及皮肤基底层表达。虽然c-Met被认为不在正常肺、甲状腺或胰腺组织中表达，但已在这些组织的肿瘤中检测到c-Met。c-Met原癌基因编码一种1408个氨基酸的糖蛋白，代表了一种新型受体酪氨酸激酶（RTK）家族的原型成员，包括Ron、Sea和Sex。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

HGFα 抗体 (H-10) 参考文献:

  1. HGF/SF：促进乳腺生长, 形态发生和发育的强效细胞因子。  |  Niranjan, B., et al. 1995. Development. 121: 2897-908. PMID: 7555716
  2. 尿激酶对原肝细胞生长因子（pro-HGF）的生物活化是由化学反应控制的。  |  Naldini, L., et al. 1995. J Biol Chem. 270: 603-11. PMID: 7822285
  3. Met/HGF 受体在人类骨肉瘤中过度表达，并通过旁分泌或自分泌回路激活。  |  Ferracini, R., et al. 1995. Oncogene. 10: 739-49. PMID: 7862451
  4. 组织损伤时肝细胞生长因子的蛋白水解活化。  |  Miyazawa, K., et al. 1994. J Biol Chem. 269: 8966-70. PMID: 8132634
  5. 人类乳腺癌中肝细胞生长因子和受体（Met）的共表达。  |  Tuck, AB., et al. 1996. Am J Pathol. 148: 225-32. PMID: 8546209
  6. 肝细胞生长因子（HGF）受体蛋白酪氨酸激酶家族成员--细胞海受体的表达和成熟。  |  Huff, JL., et al. 1996. Oncogene. 12: 299-307. PMID: 8570207
  7. 与肝细胞生长因子受体 MET 同源的跨膜蛋白家族。  |  Maestrini, E., et al. 1996. Proc Natl Acad Sci U S A. 93: 674-8. PMID: 8570614

订购信息

产品名称产品编号规格价格数量收藏夹

HGFα 抗体 (H-10)

sc-374422
200 µg/ml
RMB2377.00

HGFα (H-10): m-IgG Fc BP-HRP 套装

sc-529741
200 µg Ab; 10 µg BP
RMB2662.00

HGFα (H-10): m-IgGκ BP-HRP 套装

sc-522733
200 µg Ab, 40 µg BP
RMB2662.00

HGFα (H-10): m-IgG1 BP-HRP 套装

sc-543663
200 µg Ab; 20 µg BP
RMB2662.00

HGFα 抗体 (H-10) AC

sc-374422 AC
500 µg/ml, 25% agarose
RMB3129.00

HGFα 抗体 (H-10) HRP

sc-374422 HRP
200 µg/ml
RMB2377.00

HGFα 抗体 (H-10) FITC

sc-374422 FITC
200 µg/ml
RMB2482.00

HGFα 抗体 (H-10) PE

sc-374422 PE
200 µg/ml
RMB2580.00

HGFα 抗体 (H-10) Alexa Fluor® 488

sc-374422 AF488
200 µg/ml
RMB2685.00

HGFα 抗体 (H-10) Alexa Fluor® 546

sc-374422 AF546
200 µg/ml
RMB2685.00

HGFα 抗体 (H-10) Alexa Fluor® 594

sc-374422 AF594
200 µg/ml
RMB2685.00

HGFα 抗体 (H-10) Alexa Fluor® 647

sc-374422 AF647
200 µg/ml
RMB2685.00

HGFα 抗体 (H-10) Alexa Fluor® 680

sc-374422 AF680
200 µg/ml
RMB2685.00

HGFα 抗体 (H-10) Alexa Fluor® 790

sc-374422 AF790
200 µg/ml
RMB2685.00

HGFα (H-10) 中和勝肽

sc-374422 P
100 µg/0.5 ml
RMB511.00

What application is the blocking peptide sc-374422 P appropriate for?

Asked by: Randy McDonald
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Great IHC stainingTest sample received very quickly, IHC staining performance is great in murine colon sections
Date published: 2017-12-06
Rated 5 out of 5 by from L02 cellit works well in liver normal cell L02 in WB with 1:500 dilution
Date published: 2017-11-22
Rated 5 out of 5 by from HGFα antibodyThe HGFα antibody is suitable for a variety of experimental methods, IHC diluted with 1/200, dyeing signal is very strong.
Date published: 2017-10-24
Rated 5 out of 5 by from Strong bandStrong band obtained in western blot analysis of HGF alpha expression in human kidney tissue extract.
Date published: 2017-05-28
Rated 5 out of 5 by from clean bandsUsed this antibody in WB in mouse cells and got clean bands for dilution 1:600.
Date published: 2017-04-15
Rated 5 out of 5 by from Excellent productThe HGF alpha Antibody (H-10): sc-374422 works very well for immunohistochemistry with formalin fixed, paraffin-embedded tissue sections, showing staining pattern localized to the cytoplasm.
Date published: 2017-02-02
Rated 4 out of 5 by from easy to detect hgf alpha in tumor cellsI use it to detect endogenous HGF alpha expression in a549 lung cancer cells and it works good, although higher concentration is needed.
Date published: 2017-01-24
Rated 5 out of 5 by from reliableImmunoperoxidase staining of paraffin embedded human kidney sections using 1:100 dilution,the work is very good and reliable.
Date published: 2016-12-18
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HGFα抗体 (H-10) is rated 4.9 out of 5 by 10.
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