Date published: 2025-9-7

021-6093-6350

SCBT Portrait Logo
Seach Input

HFE 抗体 (C-12): sc-514405

5.0(3)
寫評論提問

说明书
  • HFE 抗体 C-12 是小鼠单克隆 IgG1 κ,HFE抗体, 在3篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列210-263位于human物种的HFE的内部区域内
  • HFE 抗体 (C-12) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 HFE
  • 抗HFE抗体(C-12)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取HFE (C-12): sc-514405免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgGκ BP-HRP是HFE Antibody (C-12) 适用于 WB 应用。 的首选辅助检测试剂,这些试剂现与HFE Antibody (C-12) 打包提供(请参阅下面的订购信息)。

快捷链接

関連項目

HFE抗体(C-12)是一种IgG1 κ小鼠单克隆HFE抗体(也称为HFE抗体),可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的HFE蛋白。HFE抗体(C-12)既有非偶联的抗HFE抗体形式,也有多种偶联形式的抗HFE抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。血色病的特点包括肝硬化、糖尿病、皮肤黑色素沉着和心力衰竭。由于血色病在诊断时是一种相对容易治疗的疾病,因此这是一种可预防的癌症。遗传性血色病中缺陷的HFE蛋白通常在十二指肠隐窝肠细胞中表达,具有独特的细胞内定位。在胎盘中,HFE蛋白与转铁蛋白受体(TfR)共定位并形成稳定关联,为HFE蛋白与铁转运之间的联系提供了依据。免疫细胞化学显示,HFE蛋白和TfR均在隐窝肠细胞中表达。Western印迹显示,与人类胎盘中的情况一样,隐窝肠细胞中的HFE蛋白与TfR和β2-微球蛋白物理相关。有人提出,HFE在细胞中具有两种相互排斥的活性:抑制铁的摄取或抑制铁的释放,血清转铁蛋白饱和度和血清转铁蛋白受体浓度之间的平衡决定了这两种功能中哪一种占主导地位。编码HFE的基因定位于人类染色体6p21.3。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

HFE 抗体 (C-12) 参考文献:

  1. HFE 在铁代谢, 遗传性血色素沉着病, 慢性病贫血和继发性铁过载中的作用。  |  Townsend, A. and Drakesmith, H. 2002. Lancet. 359: 786-90. PMID: 11888608
  2. 人类铁蛋白 H 和 L 序列位于十条不同的染色体上。  |  McGill, JR., et al. 1987. Hum Genet. 76: 66-72. PMID: 3032771
  3. 人类铁蛋白 'H '亚基的基因存在于一些人类染色体上。  |  Cragg, SJ., et al. 1985. Hum Genet. 71: 108-12. PMID: 3862645
  4. 人类十二指肠隐窝肠细胞中的 HFE 蛋白与转铁蛋白受体的关联。  |  Waheed, A., et al. 1999. Proc Natl Acad Sci U S A. 96: 1579-84. PMID: 9990067

订购信息

产品名称产品编号规格价格数量收藏夹

HFE 抗体 (C-12)

sc-514405
200 µg/ml
RMB2377.00

HFE (C-12): m-IgG Fc BP-HRP 套装

sc-531113
200 µg Ab; 10 µg BP
RMB2662.00

HFE (C-12): m-IgGκ BP-HRP 套装

sc-524712
200 µg Ab, 40 µg BP
RMB2662.00

HFE 抗体 (C-12) AC

sc-514405 AC
500 µg/ml, 25% agarose
RMB3129.00

HFE 抗体 (C-12) HRP

sc-514405 HRP
200 µg/ml
RMB2377.00

HFE 抗体 (C-12) FITC

sc-514405 FITC
200 µg/ml
RMB2482.00

HFE 抗体 (C-12) PE

sc-514405 PE
200 µg/ml
RMB2580.00

HFE 抗体 (C-12) Alexa Fluor® 488

sc-514405 AF488
200 µg/ml
RMB2685.00

HFE 抗体 (C-12) Alexa Fluor® 546

sc-514405 AF546
200 µg/ml
RMB2685.00

HFE 抗体 (C-12) Alexa Fluor® 594

sc-514405 AF594
200 µg/ml
RMB2685.00

HFE 抗体 (C-12) Alexa Fluor® 647

sc-514405 AF647
200 µg/ml
RMB2685.00

HFE 抗体 (C-12) Alexa Fluor® 680

sc-514405 AF680
200 µg/ml
RMB2685.00

HFE 抗体 (C-12) Alexa Fluor® 790

sc-514405 AF790
200 µg/ml
RMB2685.00

Hi, do you sell or know about an antibody that recognizes H63D mutated region of the HFE molecule (amino acid 63 in the HFE-alpha1 domaine: Histidine versus Aspartate exchange)?

Asked by: Anonymous
Thank you for your question. We currently offer two antibodies specific against HFE, which are Anti-HFE Antibody (C-12): sc-514405 and Anti-HFE Antibody (44-H): sc-130375. Unfortunately, neither recognise any particular mutations specifically.
Answered by: Tech Support Europe
Date published: 2022-09-05

Hello, HFE is a transmembrane protein. Where does the antibody bind? On the intracellular part or the extracellular part of the protein?

Asked by: We4ver
Thank you for your question. HFE Antibody (C-12): sc-514405 was raised against amino acids 210-263 mapping within an internal region of HFE of human origin. This sequence corresponds to the protein's extracellular domain.
Answered by: Tech Support Europe
Date published: 2021-02-23

Hello. What secondary antibody (for Western blot) would be best for these: 1) HFE Antibody (C-12): sc-514405, 2) CD71 Antibody (3B8 2A1): sc-32272, 3)TFR2 Antibody (9F8 1C11): sc-32271, 4) β-2-Microglobulin Antibody (BBM.1): sc-13565 ?

Asked by: AnnaMargret
Thank you for your question. I would suggest this secondary reagent for use with all these antibodies: mouse IgG kappa binding protein (m-IgGκ BP) , sc-516102 https://www.scbt.com/p/m-igg-kappa-bp-hrp?requestFrom=search
Answered by: Tech Service
Date published: 2019-12-17

I need to use an antibody to detect the protein in samples of canine origin. Do you know if HFE (C-12): sc-514405 mouse monoclonal antibody will work?

Asked by: TinTin
Thank you for your inquiry. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity. We are available by phone, e-mail or chat.
Answered by: Technical Support
Date published: 2017-03-01
  • y_2025, m_8, d_30, h_9CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_4
  • loc_zh_CN, sid_514405, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 99ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Good choice for Western BlotAntibody works consistently in HeLa and A-431 whole cell lysates. -SCBT QC
Date published: 2023-09-14
Rated 5 out of 5 by from it works wellI have done in breast cancer cells, the band is clear, soon appeared, the effect is good.
Date published: 2017-11-09
  • y_2025, m_8, d_30, h_9
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_1, tr_2
  • loc_zh_CN, sid_514405, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 8ms
  • REVIEWS, PRODUCT
HFE 抗体 (C-12) is rated 5.0 out of 5 by 3.
  • y_2025, m_8, d_30, h_9
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_1, tr_2
  • loc_zh_CN, sid_514405, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 99ms
  • REVIEWS, PRODUCT