Date published: 2025-9-10
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Hep B sAg Antibody (1023) - Western Blotting - Image 17233
乙型肝炎表面抗原抗体 (1023): sc-53299. 重组乙型肝炎表面抗原的 Western 印迹分析.
Hep B sAg Antibody (1023) - Western Blotting - Image 17233
乙型肝炎表面抗原抗体 (1023): sc-53299. 重组乙型肝炎表面抗原的 Western 印迹分析.
乙型肝炎表面抗原抗体 (1023): sc-53299. 重组乙型肝炎表面抗原的 Western 印迹分析.

Hep B sAg 抗体 (1023): sc-53299

2.7(3)
寫評論提問

说明书
  • Hep B sAg 抗体 1023 是小鼠单克隆 IgG1 κ，Hep B sAg抗体, 在9篇文献中引用，规格为200 µg/ml
  • 针对重组Hep B sAg
  • Hep B sAg 抗体 (1023) 推荐用于WB 和 IP，检测Hep B 来源的 surface antigen
  • 抗Hep B sAg抗体（1023）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Hep B sAg (1023): sc-53299的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是Hep B sAg Antibody (1023) for WB applications. 的首选辅助检测试剂，这些试剂现与Hep B sAg Antibody (1023) 打包提供（请参阅下面的订购信息）。

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描述
说明书与实验方案
研究信息

関連項目

额外Hepatitis B Protein抗体，包括Hep B cAg, Hep B eAg, Hep B Pol, Hep B preS1, Hep B preS2, Hep B sAg和Hep B xAg

Hep B sAg抗体（1023）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）和免疫沉淀（IP）检测Hep B来源的Hep B sAg蛋白。Hep B sAg单克隆抗体（1023）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。乙型肝炎病毒（HBV）是肝病毒家族的一员，以引起肝炎症、黄疸和严重时导致死亡而闻名。乙型肝炎表面抗原蛋白（sAg）的一个关键方面是其在感染期间释放到血液中的表面抗原的作用，它作为HBV存在的早期标志物，通常甚至在临床症状出现之前就可以检测到。早期检测对于及时诊断和管理感染至关重要，因为乙型肝炎与罹患肝细胞癌的风险显著增加有关，全球有超过2.5亿人受到影响。HBV基因组部分为双链结构，长度约为3.2千碱基，可编码多种蛋白质，包括HBx蛋白，后者在病毒复制和发病机理中起作用。使用抗乙肝表面抗原抗体（1023）可有效研究HBV感染动态及其对肝脏健康的影响。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Hep B sAg 抗体 (1023) 参考文献:

  1. 编码主要 HBsAg 基因的遗传改变：对来自单克隆抗体治疗的肝移植患者的复发性 HBsAg 进行 DNA 和免疫学分析。  |  McMahon, G., et al. 1992. Hepatology. 15: 757-66. PMID: 1568715
  2. HBsAg 阳性患者肝移植后的被动免疫预防。  |  Samuel, D., et al. 1991. Lancet. 337: 813-5. PMID: 1672913
  3. 慢性乙型肝炎病毒感染者血清 HBsAg 清除延迟的发生率, 决定因素和意义：一项前瞻性研究。  |  Liaw, YF., et al. 1991. Hepatology. 13: 627-31. PMID: 2010157
  4. 在分化的人类肝癌衍生细胞系中可控合成 HBsAg。  |  Aden, DP., et al. 1979. Nature. 282: 615-6. PMID: 233137
  5. 接受聚乙二醇干扰素 alfa-2a 治疗的非活动性乙型肝炎表面抗原携带者的乙型肝炎表面抗原清除率。  |  Li, MH., et al. 2016. World J Hepatol. 8: 637-43. PMID: 27239256
  6. 在乙型肝炎病毒和原发性肝细胞癌流行地区对新生儿普遍接种小剂量乙型肝炎疫苗的试点研究。  |  Sun, TT., et al. 1986. J Cell Physiol Suppl. 4: 83-90. PMID: 3018008
  7. 乙型肝炎表面抗体在使用聚乙二醇干扰素治疗实现乙型肝炎表面抗原消失的患者中乙型肝炎表面抗原血清转换中的作用。  |  Gao, N., et al. 2022. J Viral Hepat. 29: 899-907. PMID: 35871534
  8. 高效实施乙型肝炎表面抗原确证中和试验。  |  Yonezawa, H., et al. 2024. J Infect Chemother. 30: 29-33. PMID: 37690522
  9. 全球 HBsAg 亚型的分布情况。  |  Couroucé-Pauty, AM., et al. 1983. Vox Sang. 44: 197-211. PMID: 6845678
  10. 乙型肝炎病毒的免疫发病机制。  |  Chisari, FV. and Ferrari, C. 1995. Annu Rev Immunol. 13: 29-60. PMID: 7612225
  11. HBsAg（-）, HBcAb（+）, HBIgM（-）器官捐献者传播乙型肝炎的风险。  |  Wachs, ME., et al. 1995. Transplantation. 59: 230-4. PMID: 7839446
  12. 使用一组单克隆抗体对新型乙型肝炎疫苗的抗原性和免疫原性进行研究。  |  Waters, JA., et al. 1998. J Med Virol. 54: 1-6. PMID: 9443102

订购信息

产品名称产品编号规格价格数量收藏夹

Hep B sAg 抗体 (1023)

sc-53299
200 µg/ml
RMB2377.00

Hep B sAg (1023): m-IgG Fc BP-HRP 套装

sc-526790
200 µg Ab; 10 µg BP
RMB2662.00

Hep B sAg (1023): m-IgG1 BP-HRP 套装

sc-532163
200 µg Ab; 20 µg BP
RMB2662.00

Hep B sAg 抗体 (1023) AC

sc-53299 AC
500 µg/ml, 25% agarose
RMB3129.00

Hep B sAg 抗体 (1023) HRP

sc-53299 HRP
200 µg/ml
RMB2377.00

Hep B sAg 抗体 (1023) FITC

sc-53299 FITC
200 µg/ml
RMB2482.00

Hep B sAg 抗体 (1023) PE

sc-53299 PE
200 µg/ml
RMB2580.00

Hep B sAg 抗体 (1023) Alexa Fluor® 488

sc-53299 AF488
200 µg/ml
RMB2685.00

Hep B sAg 抗体 (1023) Alexa Fluor® 546

sc-53299 AF546
200 µg/ml
RMB2685.00

Hep B sAg 抗体 (1023) Alexa Fluor® 594

sc-53299 AF594
200 µg/ml
RMB2685.00

Hep B sAg 抗体 (1023) Alexa Fluor® 647

sc-53299 AF647
200 µg/ml
RMB2685.00

Hep B sAg 抗体 (1023) Alexa Fluor® 680

sc-53299 AF680
200 µg/ml
RMB2685.00

Hep B sAg 抗体 (1023) Alexa Fluor® 790

sc-53299 AF790
200 µg/ml
RMB2685.00

Hello. It is known that HBsAg surface antigen has three structures. I would like to know which part to target Pre S1, Pre S2, and S parts. More precisely, I want to know which sequence is attached to a specific site. My target is HBsAg on cell membrane

Asked by: 0honey
Thank you for your question. sc-53299, Hep B sAg Antibody (1023) is recommended for detection of surface antigen of Hep B origin by WB and IP. We will recommend sc-57761, sc-57762 and sc-73587 for the detection of preS1 region of Hep B origin by WB, IP, IF and IHC(P). https://www.scbt.com/browse/hep-b-pres1-Antibodies/_/N-1m22gpy If you wants to detect the Pre S2, we will reommend Hep B preS2 Antibody (S 26), sc-23944. https://www.scbt.com/browse/hep-b-pres2-Antibodies/_/N-zsnsbw https://www.scbt.com/browse/hep-b-sag-Antibodies/_/N-soixtz
Answered by: Ling Chen
Date published: 2023-01-13

Which geneotype and serotype of HBV was the Ab raised against? I'm looking for something that recognises genotype D serotype ayw HBsAg

Asked by: BeckyM
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2024-03-28

请问SC-53299这个抗体他能检测到HBsAg大中小三种抗原吗?因为看到说明书上的效果图是20+KD，其大小是一个小S，那么小S作为组成中S和大S的一部分，理论上中S和大S应该也可以检测到，但是效果图上没有呈现，所以想请教一下，这个抗体是否可以检测到大中小三种HBV表面抗原。

Asked by: luoluo
Thank you for your question. The molecular weight of the protein is about 28 kDa according to our current data. 感谢您的咨询.目前我们这个货号的抗体，根据已有数据显示，只能检测到蛋白分子量为28左右。
Answered by: Anonymous
Date published: 2021-12-07

检测HepG2.2.15细胞内的表面抗原可以用sc-53299吗？另外，怎么申请试用装？谢谢！

Asked by: fanfanhx
感谢您的提问。关于抗体的反应性，请联系我们的技术支持部门。我们会比对我们的数据以及您提供的信息，为您具体解答。亚洲技术支持电话(+86) 021-60936350、邮箱[email protected]。申请小样也请联系技术支持。 Thank you for your question. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about reactivity. To receive a testing sample, please also contact Technical Service.
Answered by: Technical Support 14
Date published: 2017-06-13

What is the recommended secondary reagent to use with Hep B sAg (1023): sc-53299 for Western blot?

Asked by: jenni
We recommend using one our exclusive Mouse IgG Binding Proteins (m-IgGκ BP) conjugated to HRP as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 3
Date published: 2017-01-27

我的目的蛋白是质粒表达载体在293细胞表达的乙肝表面抗原S蛋白。请问这个货号的产品能不能反应？ 产品是否有现货？下订单后，多久能送到福州？ 谢谢！

Asked by: xiaochunfu
感谢您的提问。Hep B sAg Antibody (1023): sc-53299推荐用于检测Hep B的表面抗原。您可以联系我们的技术支持部门，我们会具体分析您的蛋白序列以确定抗体反应性。技术支持部门：电话(+86) 021-60936351，邮箱[email protected]。产品购买的相关问题，您也可以一并咨询技术支持。 Thank you for your question. Hep B sAg Antibody (1023): sc-53299 is recommended for detection of surface antigen of Hep B. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about reactivity.
Answered by: Technical Support 14
Date published: 2017-01-11
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Rated 1 out of 5 by from Doesn't work for meTried this antibody on many blots with a wide variety of recombinant HBs and cell samples, and this antibody didn't produce any signal. By comparison, the preS1 antibody sc57761 worked fine.
Date published: 2018-09-06
Rated 5 out of 5 by from Good choice for Hep B sAgStrong distinct band in WB of recombinant hepatitis B surface antigen. Referenced in literature for IHC on HCC cell lines
Date published: 2016-12-22
Rated 2 out of 5 by from Robust band seen in WB using recombinantRobust band seen in WB using recombinant hepatitis B surface antigen. -SCBT QC
Date published: 2013-01-06
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Hep B sAg 抗体 (1023) is rated 2.7 out of 5 by 3.
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