Date published: 2025-9-7
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Hemogen Antibody (D-4) - Western Blotting - Image 294830
血原抗体 (D-4): sc-393386. K-562 (A), Jurkat (B), HEL 92. 1. 7 (C) 和 MEG-01 (D) 核提取物中血原表达的 Western 印迹分析.
血原抗体 (D-4): sc-393386. K-562 (A), Jurkat (B), HEL 92. 1. 7 (C) 和 MEG-01 (D) 核提取物中血原表达的 Western 印迹分析.

Hemogen 抗体 (D-4): sc-393386

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说明书
  • Hemogen抗体(D-4)是小鼠单克隆IgG1 (kappa轻链)，规格为200 µg/ml
  • 免疫原氨基酸序列1-300位于human物种的Hemogen的N-terminus
  • 推荐用于 mouse, rat 和 human 来源的Hemogen WB, IP, IF, IHC(P) 和 ELISA检测
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 Hemogen 抗体 (D-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Hemogen 抗体 (D-4) 组合成套装提供。 (请参阅下面的订购信息)。

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    描述
    基因信息
    说明书与实验方案
    研究信息

    関連項目

    Hemogen（造血基因蛋白，红系分化相关基因蛋白）是一种由人类基因HEMGN编码的含有484个氨基酸的蛋白质。Hemogen是一种在造血前体细胞中表达的核蛋白，可在CD34+和K-562白血病细胞系中检测到。它也在骨髓、睾丸、胸腺和甲状腺肿瘤、非霍奇金淋巴瘤、各种白血病细胞系、白血病患者的外周血单个核细胞(PBMCs)和骨髓单个核细胞(BMMCs)中表达。Hemogen在12-O-十四烷酰佛波醇-13-乙酸酯(TPA)诱导的K-562细胞巨核细胞分化过程中下调（在蛋白质水平）。它可被促有丝分裂原在正常PBMCs中上调。

    仅限研究使用。不适用于诊断和治疗用途。

    订购信息

    产品名称产品编号规格价格数量收藏夹

    Hemogen 抗体 (D-4)

    sc-393386
    200 µg/ml
    		RMB2377.00

    Hemogen (D-4): m-IgG Fc BP-HRP 套装

    sc-538202
    200 µg Ab; 10 µg BP
    		RMB2662.00

    Hemogen (D-4): m-IgGκ BP-HRP 套装

    sc-535770
    200 µg Ab; 40 µg BP
    		RMB2662.00

    Hemogen (D-4): m-IgG1 BP-HRP 套装

    sc-545575
    200 µg Ab; 20 µg BP
    		RMB2662.00

    Can Hemogen (D-4): sc-393386 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

    Asked by: Randy McDonald
    Thank you for your question. Yes, Hemogen (D-4): sc-393386 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
    Answered by: Technical Support
    Date published: 2017-03-01
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    Rated 1 out of 5 by from Nothing is detectedThis antibody detected no band at the right position except many background bands. The mRNA is induced substantially. Livers are from infected mice.
    Date published: 2021-06-04
    Rated 5 out of 5 by from Produced positive Western blot data of HemogenProduced positive Western blot data of Hemogen expression in K-562, Jurkat, HEL 92.1.7 and MEG-01 nuclear extracts. -SCBT QC
    Date published: 2014-11-21
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    Hemogen 抗体 (D-4) is rated 3.0 out of 5 by 2.
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