Date published: 2025-9-8
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
Heme Oxygenase 2 Antibody (B-3) - Western Blotting - Image 15217
Heme Oxygenase 2 Antibody (B-3): sc-17786. Western blot analysis of Heme Oxygenase 2 expression in K-562 (A), ECV304 (B), Hel 92.1.7 (C) and NIH/3T3 (D) whole cell lysates.
Heme Oxygenase 2 Antibody (B-3): sc-17786. Western blot analysis of Heme Oxygenase 2 expression in K-562 (A), ECV304 (B), Hel 92.1.7 (C) and NIH/3T3 (D) whole cell lysates.

Heme Oxygenase 2 抗体 (B-3): sc-17786

4.8(8)
寫評論提問

说明书
  • Heme Oxygenase 2 抗体 B-3 是小鼠单克隆 IgG1 κ，Heme Oxygenase 2抗体, 在7篇文献中引用，规格为200 µg/ml
  • 免疫human来源Heme Oxygenase 2近C-terminus的239-311氨基酸
  • Heme Oxygenase 2 抗体 (B-3) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Heme Oxygenase 2
  • 抗Heme Oxygenase 2抗体（B-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Heme Oxygenase 2 (B-3): sc-17786的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 Heme Oxygenase 2 抗体 (B-3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Heme Oxygenase 2 抗体 (B-3) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 Heme Oxygenase 抗体 包括 Heme Oxygenase 1, Heme Oxygenase 2和Heme Oxygenase 3

Heme Oxygenase 2 Antibody (B-3) 是一种小鼠单克隆 IgG1 kappa 轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的 Heme Oxygenase 2 (HMOX2)。抗HMOX2抗体（B-3）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。HMOX2在脑内一氧化碳（CO）的产生中起着至关重要的作用，CO在脑内作为神经递质，影响各种生理过程。HMOX2在哺乳动物组织中具有固有表达，这使其与HMOX1区分开来，后者在压力条件下具有高度诱导性。CO信号通路与一氧化氮的信号通路密切相关，这凸显了HMOX2在维持细胞稳态和神经保护方面的重要性。HMOX2参与血红素代谢，生成胆绿素和游离铁，这对细胞抗氧化防御机制至关重要。了解HMOX2的功能对于阐明其在神经生物学中的作用以及其在神经退行性疾病中的潜在治疗应用至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Heme Oxygenase 2 抗体 (B-3) 参考文献:

  1. 血红素加氧酶的发育生物学。  |  Rodgers, PA. and Stevenson, DK. 1990. Clin Perinatol. 17: 275-91. PMID: 2196131
  2. 血红素加氧酶-2（HO-2）作为治疗靶点：激活剂和抑制剂。  |  Intagliata, S., et al. 2019. Eur J Med Chem. 183: 111703. PMID: 31550661
  3. 血红素加氧酶：功能, 多样性, 调节机制和临床应用。  |  Maines, MD. 1988. FASEB J. 2: 2557-68. PMID: 3290025
  4. 血红素加氧酶-2对小鼠脑出血后神经的保护作用  |  Zhang, Z., et al. 2021. J Neuropathol Exp Neurol. 80: 457-466. PMID: 33870420
  5. 血红素加氧酶-2（HEME oxygenase-2，HO-2）在人类胚胎肾细胞中结合并缓冲易溶的铁血黄素。  |  Hanna, DA., et al. 2022. J Biol Chem. 298: 101549. PMID: 34973332
  6. 血红素向血红素加氧酶-2的输送涉及甘油醛-3-磷酸脱氢酶。  |  Dai, Y., et al. 2022. Biol Chem. 403: 1043-1053. PMID: 36302634
  7. 血红素加氧酶-2通过防止炎症减轻动脉粥样硬化。  |  Wang, Z., et al. 2023. Curr Res Pharmacol Drug Discov. 4: 100141. PMID: 36593926
  8. 小鼠血红素加氧酶-1 基因的分离和特征描述。血红素或重金属诱导需要远端 5' 序列。  |  Alam, J., et al. 1994. J Biol Chem. 269: 1001-9. PMID: 8288554
  9. 血红素加氧酶系统：第二信使气体的调节器。  |  Maines, MD. 1997. Annu Rev Pharmacol Toxicol. 37: 517-54. PMID: 9131263
  10. 从大鼠大脑中分离并鉴定编码血红蛋白血红素加氧酶-3的 cDNA。  |  McCoubrey, WK., et al. 1997. Eur J Biochem. 247: 725-32. PMID: 9266719
  11. 一氧化氮和一氧化碳：作为神经信使的平行角色。  |  Snyder, SH., et al. 1998. Brain Res Brain Res Rev. 26: 167-75. PMID: 9651518
  12. 血红素加氧酶-1 衍生物一氧化碳有助于抑制体内急性高血压反应。  |  Motterlini, R., et al. 1998. Circ Res. 83: 568-77. PMID: 9734480

订购信息

产品名称产品编号规格价格数量收藏夹

Heme Oxygenase 2 抗体 (B-3)

sc-17786
200 µg/ml
RMB2377.00

Heme Oxygenase 2 (B-3): m-IgG Fc BP-HRP 套装

sc-528313
200 µg Ab; 10 µg BP
RMB2662.00

Heme Oxygenase 2 (B-3): m-IgGκ BP-HRP 套装

sc-520657
200 µg Ab, 40 µg BP
RMB2662.00

Heme Oxygenase 2 (B-3): m-IgG1 BP-HRP 套装

sc-542861
200 µg Ab; 20 µg BP
RMB2662.00

Heme Oxygenase 2 抗体 (B-3) AC

sc-17786 AC
500 µg/ml, 25% agarose
RMB3129.00

Heme Oxygenase 2 抗体 (B-3) HRP

sc-17786 HRP
200 µg/ml
RMB2377.00

Heme Oxygenase 2 抗体 (B-3) FITC

sc-17786 FITC
200 µg/ml
RMB2482.00

Heme Oxygenase 2 抗体 (B-3) PE

sc-17786 PE
200 µg/ml
RMB2580.00

Heme Oxygenase 2 抗体 (B-3) Alexa Fluor® 488

sc-17786 AF488
200 µg/ml
RMB2685.00

Heme Oxygenase 2 抗体 (B-3) Alexa Fluor® 546

sc-17786 AF546
200 µg/ml
RMB2685.00

Heme Oxygenase 2 抗体 (B-3) Alexa Fluor® 594

sc-17786 AF594
200 µg/ml
RMB2685.00

Heme Oxygenase 2 抗体 (B-3) Alexa Fluor® 647

sc-17786 AF647
200 µg/ml
RMB2685.00

Heme Oxygenase 2 抗体 (B-3) Alexa Fluor® 680

sc-17786 AF680
200 µg/ml
RMB2685.00

Heme Oxygenase 2 抗体 (B-3) Alexa Fluor® 790

sc-17786 AF790
200 µg/ml
RMB2685.00

I am using Heme Oxygenase 2 (B-3): sc-17786 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: TinTin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
  • y_2025, m_9, d_7, h_4CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_zh_CN, sid_17786, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 104ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from a good antibody for WBThis antibody recognizes a band of predicted molecular weight (1:500 dilution, 30 ug HeLa total extract).
Date published: 2017-08-24
Rated 5 out of 5 by from very specificThis antibody is very specific for western blot. the band is clear.
Date published: 2017-07-30
Rated 4 out of 5 by from WBI have got one clear band at 45 kd, much bigger than expected 36kd in THP1 lysate. So I am not sure whether this antibody could really recognize HMOX2 antigen.
Date published: 2017-04-13
Rated 5 out of 5 by from Highly potent and very specific antibodyThis monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was strong detection of the ~36 kDa target protein in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested.
Date published: 2016-11-10
Rated 5 out of 5 by from Published WB data in HepG2 cell lysates usingPublished WB data in HepG2 cell lysates using 1:2500 dilution. -SCBT Publication Review
Date published: 2015-05-11
Rated 5 out of 5 by from Clean results with BClean results with B-3 in western blot with human monocytes and macrophages.
Date published: 2015-05-10
Rated 5 out of 5 by from Great immunoperoxidase cytoplasmic and membraneGreat immunoperoxidase cytoplasmic and membrane staining in formalin fixed, paraffin-embedded human gall bladder tissue. -SCBT QC
Date published: 2015-03-05
Rated 5 out of 5 by from Great antibody for WB; worked well with KGreat antibody for WB; worked well with K-562, ECV304, Hel 92.1.7 and NIH/3T3 whole cell lysates. -SCBT QC
Date published: 2015-02-28
  • y_2025, m_9, d_7, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_8
  • loc_zh_CN, sid_17786, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 20ms
  • REVIEWS, PRODUCT
Heme Oxygenase 2 抗体 (B-3) is rated 4.8 out of 5 by 8.
  • y_2025, m_9, d_7, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_8
  • loc_zh_CN, sid_17786, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 105ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。