Date published: 2025-9-7

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Histone Deacetylase 9 (HDAC9) 抗体 (B-1): sc-398003

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说明书
  • Histone Deacetylase 9 (HDAC9) 抗体 B-1 是小鼠单克隆 IgM κ,Histone Deacetylase 9 (HDAC9)抗体, 在12篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的HDAC9的N-terminus的氨基酸12-39之间
  • 推荐用于 mouse, rat 和 human 来源的HDAC9 isoforms 1-4 WB, IP, IF 和 ELISA检测
  • 关于如何获取Histone Deacetylase 9 (HDAC9) (B-1): sc-398003免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP是Histone Deacetylase 9 (HDAC9) Antibody (B-1) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现可与Histone Deacetylase 9 (HDAC9) Antibody (B-1) 搭配使用(请参阅下面的订购信息)。有关其它 m-IgGκ BP 结合物,请参阅我们完整的小鼠 IgG 结合蛋白列表。

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関連項目

HDAC9抗体(B-1)是一种IgM κ小鼠单克隆HDAC9抗体(也称为组蛋白去乙酰化酶9抗体),可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的HDAC9蛋白。HDAC9抗体(B-1)为非偶联型抗HDAC9抗体。在完整细胞中,DNA与组蛋白和其他核蛋白紧密结合形成染色质。染色质重塑是转录调控的关键组成部分,而核小体组蛋白的乙酰化是这种重塑的主要来源。组蛋白氨基末端尾部结构域中赖氨酸残基的乙酰化导致核小体构象的变构变化和DNA对转录因子的可接近性增加。几种哺乳动物蛋白具有核组蛋白乙酰化酶活性,包括GCN5、PCAF(p300/CBP相关因子)、p300/CBP、HAT1和TFIID亚基TAF II p250。相反,组蛋白的去乙酰化与转录沉默相关。组蛋白去乙酰化酶(HDAC)包括HDAC1-9。HDAC9和HDAC9a是HDAC9的两种选择性剪接异构体。HDAC9a比HDAC9短132个氨基酸,但两种异构体都含有HDAC催化结构域,仍具有去乙酰化酶活性,并抑制肌细胞增强因子2介导的转录。HDAC9和HDAC9a在脑、骨骼肌、肾、胎盘和胰腺中表达。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Histone Deacetylase 9 (HDAC9) 抗体 (B-1) 参考文献:

  1. 组蛋白去乙酰化酶 HDAC9 的克隆和表征。  |  Zhou, X., et al. 2001. Proc Natl Acad Sci U S A. 98: 10572-7. PMID: 11535832
  2. 酮代谢产物β-羟基丁酸下调HDAC9可抑制血管钙化。  |  Lan, Z., et al. 2022. J Pathol. 258: 213-226. PMID: 35894849
  3. HDAC9介导的上皮细胞G2/M期周期性阻滞导致雄性小鼠的肾纤维化。  |  Zhang, Y., et al. 2023. Nat Commun. 14: 3007. PMID: 37230975
  4. 中风通过诱导HDAC9依赖性的HIF-1和Sp1去乙酰化,促进TfR1转录和GPX4减少,从而决定铁死亡神经元死亡。  |  Sanguigno, L., et al. 2023. Int J Biol Sci. 19: 2695-2710. PMID: 37324938
  5. HDAC9抑制剂作为中风的新疗法。  |  Markus, HS. 2023. Stroke. 54: 3182-3189. PMID: 37942644
  6. 抑制HIF-1α诱导的HDAC9有助于venetoclax和MENIN抑制剂在KMT2Ar AML中的协同作用。  |  Ling, Q., et al. 2023. Biomark Res. 11: 105. PMID: 38053201
  7. HDAC9在椎间盘退变中的分子机制和治疗潜力。  |  Lei, M., et al. 2023. Cell Mol Biol Lett. 28: 104. PMID: 38093179
  8. 乙酰化导致的核小体结构变化  |  Bauer, WR., et al. 1994. J Mol Biol. 236: 685-90. PMID: 8114086
  9. 组蛋白乙酰化在转录因子进入核糖体 DNA 的过程中发挥了积极作用。  |  Lee, DY., et al. 1993. Cell. 72: 73-84. PMID: 8422685
  10. 酵母的转录沉默与核小体乙酰化减少有关。  |  Braunstein, M., et al. 1993. Genes Dev. 7: 592-604. PMID: 8458576
  11. 核糖体 DNA 调节人类 Hat1 乙酰转移酶的核心-活素结合亚基。  |  Verreault, A., et al. 1998. Curr Biol. 8: 96-108. PMID: 9427644
  12. 转录激活剂将组蛋白乙酰转移酶复合物导向核糖体。  |  Utley, RT., et al. 1998. Nature. 394: 498-502. PMID: 9697775

订购信息

产品名称产品编号规格价格数量收藏夹

Histone Deacetylase 9 (HDAC9) 抗体 (B-1)

sc-398003
200 µg/ml
RMB2377.00

Histone Deacetylase 9 (HDAC9) (B-1): m-IgGκ BP-HRP 套装

sc-551266
200 µg Ab; 40 µg BP
RMB2662.00

Histone Deacetylase 9 (HDAC9) (B-1) 中和勝肽

sc-398003 P
100 µg/0.5 ml
RMB511.00

请问Histone Deacetylase9(HDAC9)抗体(B-1) sc-398003,mouse lgM K。官网上放的图,二抗选用的是?

Asked by: Tutu
Thank you for your inquiry. Please contact our Technical Service Department. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website. 感谢您的提问。请联系我们的技术支持部门。亚洲技术支持电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”。
Answered by: Yanru Tao
Date published: 2023-05-12

Hi dear Santa Cruz staff, thank you for reading this message. I am working with HDAC9, and would like to have a trial sample of your HDAC9 antobody (sc-398003). If it works well for our experiment, we would love to order some afterwards. Yan

Asked by: munichyan
Thank you for the question. Our Customer Service Department will contact you asap.
Answered by: Technical Support Europe
Date published: 2022-10-27

Has this antibody been validated recently by blotting HDAC9 KO cell lysate, or ectopic HDAC9 protein?

Asked by: lingh
Thank you for your question. We have not tested this mouse monoclonal antibody, HDAC9 (B-1): sc-398003, using an HDAC9 knockout cell line. The antibody has been validated using a variety of tests, including the Western blot data published on our website in Ramos and NAMALWA whole cell lysates. We do offer a range of human specific HDAC9 CRISPR products that could be used in combination with this antibody as controls to demonstrate antibody specificity to the target protein. For example, our HDAC9 CRISPR/Cas9 KO Plasmid (h): sc-401419. https://www.scbt.com/scbt/product/hdac9-crispr-knockout-and-activation-products-h
Answered by: Technical Support
Date published: 2022-10-27

Can HDAC9 (B-1): sc-398003 monoclonal antibody be used in IHC with paraffin-embedded tissue sections?

Asked by: sweetums
Thank you for your inquiry. This antibody has not been validated in house for use in IHC(P). For any publications in this application and further details, please contact our Technical Service Department.
Answered by: Technical Support 5
Date published: 2022-10-27
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Rated 1 out of 5 by from only detected 55kda bandUsing human blood monocyte derived macrophages, 25 ug total cell lysate ran on midi gels, 1:500 dilution in milk, 1:4000 2nd Ab CST anti-mouse we could only detect a band at wrong size. The signal was also not too strong.
Date published: 2021-07-19
Rated 5 out of 5 by from works greatthe antibody works great for WB in human podocytes
Date published: 2019-11-14
Rated 3 out of 5 by from Schwache definierte Bande mit OsteoblastenNut bei Nutzung der empfohlenen Sekundär-Antikörper und Reagenzien
Date published: 2018-04-12
Rated 1 out of 5 by from this HDAC9 antibody is kind of not working wellI got a free sample of the HDAC9 antibody from Santa Cruz and tested it for Western blotting. A clean and strong band with a size around 140 kD did appear. But the band is kind of not for HDAC9. Because the antibody even cannot recognize overexpressed Flag-tagged full HDAC9, which, however, can be detected by anti-Flag or some other anti-HDAC9 antibodies. Does any other person get a similar result? Can you help to recommend a good HDAC9 antibody to detect endogenous HDAC9 by WB and IP, etc?
Date published: 2017-12-01
Rated 5 out of 5 by from 做WB好用实验室一直用这款抗体,价格实惠,使用效果好,文章已顺利发表,建议稀释倍数为1:500,推荐生物学科同仁使用。
Date published: 2017-08-07
Rated 5 out of 5 by from Specific and robust in WBGreat for Western blotting, gives nice solid bands, reliably. Highly recommend!
Date published: 2017-01-12
Rated 5 out of 5 by from Good specificity and signal strength in RamosGood specificity and signal strength in Ramos whole cell lysate. -SCBT QC
Date published: 2015-04-10
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Histone Deacetylase 9 (HDAC9) 抗体 (B-1) is rated 3.6 out of 5 by 7.
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