Date published: 2025-9-6
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Histone Deacetylase 4 (HDAC4) Antibody (A-4) - Western Blotting - Image 381025
HDAC4 抗体 (A-4) HRP: sc-46672 HRP. 在 Jurkat (A), NIH/3T3 (B), THP-1 (C) 和 MOLT-4 (D) 全细胞裂解液以及 HeLa (E) 和 Jurkat (F) 核提取物中直接进行 HDAC4 表达的 western 印迹分析.
HDAC4 抗体 (A-4) HRP: sc-46672 HRP. 在 Jurkat (A), NIH/3T3 (B), THP-1 (C) 和 MOLT-4 (D) 全细胞裂解液以及 HeLa (E) 和 Jurkat (F) 核提取物中直接进行 HDAC4 表达的 western 印迹分析.

Histone Deacetylase 4 (HDAC4) 抗体 (A-4): sc-46672

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说明书
  • Histone Deacetylase 4 (HDAC4) 抗体 A-4 是小鼠单克隆 IgG2b κ，Histone Deacetylase 4 (HDAC4)抗体, 在50篇文献中引用，规格为200 µg/ml
  • 免疫mouse物种的HDAC4的氨基酸530-631
  • Histone Deacetylase 4 (HDAC4) 抗体 (A-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 HDAC4
  • 抗Histone Deacetylase 4 (HDAC4)抗体（A-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-46672 X, 200 µg/0.1 ml)
  • 关于如何获取Histone Deacetylase 4 (HDAC4) (A-4): sc-46672的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 Histone Deacetylase 4 (HDAC4) 抗体 (A-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Histone Deacetylase 4 (HDAC4) 抗体 (A-4) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 HDAC 抗体 包括 HDAC1, HDAC10, HDAC11, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7和HDAC8

HDAC4抗体（A-4）是一种小鼠单克隆IgG2b kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的HDAC4蛋白。HDAC4 (A-4) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。HDAC4蛋白作为组蛋白去乙酰化酶，在基因表达调控中发挥着关键作用，它从组蛋白上的赖氨酸残基上移除乙酰基，导致染色质凝结和转录抑制。这一过程对于维持细胞内稳态和调节发育过程至关重要，因为HDAC4的异常表达或活性与多种疾病有关，包括癌症和神经退行性疾病。此外，HDAC4还与其它几种蛋白相互作用，例如转录因子MEF2，后者与肌肉分化及神经元功能有关，突出了其在细胞信号通路中的重要性。在研究中使用抗HDAC4抗体（A-4）可以深入了解基因表达的调节机制，以及与组蛋白乙酰化失调相关的疾病的潜在治疗靶点。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Histone Deacetylase 4 (HDAC4) 抗体 (A-4) 参考文献:

  1. 三种蛋白定义了一类与酵母 Hda1p 有关的人类组蛋白去乙酰化酶。  |  Grozinger, CM., et al. 1999. Proc Natl Acad Sci U S A. 96: 4868-73. PMID: 10220385
  2. MIR145-3p 通过靶向 HDAC4 促进多发性骨髓瘤的自噬并增强硼替佐米的敏感性。  |  Wu, H., et al. 2020. Autophagy. 16: 683-697. PMID: 31242129
  3. HDAC4抑制剂可作为抗血管衰老治疗药物。  |  Huang, C., et al. 2022. Oxid Med Cell Longev. 2022: 3087916. PMID: 35814270
  4. m6A甲基化通过ALKBH5-HDAC4-HIF1α正反馈回路调节缺氧诱导的胰腺癌糖酵解代谢。  |  Liu, X., et al. 2023. Oncogene. 42: 2047-2060. PMID: 37149664
  5. 乙酰化导致的核小体结构变化  |  Bauer, WR., et al. 1994. J Mol Biol. 236: 685-90. PMID: 8114086
  6. 组蛋白乙酰化在转录因子进入核糖体 DNA 的过程中发挥了积极作用。  |  Lee, DY., et al. 1993. Cell. 72: 73-84. PMID: 8422685
  7. 酵母的转录沉默与核小体乙酰化减少有关。  |  Braunstein, M., et al. 1993. Genes Dev. 7: 592-604. PMID: 8458576
  8. 一种与酵母转录调节因子 Rpd3p 相关的哺乳动物组蛋白去乙酰化酶。  |  Taunton, J., et al. 1996. Science. 272: 408-11. PMID: 8602529
  9. 核糖体 DNA 调节人类 Hat1 乙酰转移酶的核心-活素结合亚基。  |  Verreault, A., et al. 1998. Curr Biol. 8: 96-108. PMID: 9427644
  10. 人类 RPD3 同源物 HDAC3 的特征。  |  Emiliani, S., et al. 1998. Proc Natl Acad Sci U S A. 95: 2795-800. PMID: 9501169
  11. 转录激活剂将组蛋白乙酰转移酶复合物导向核糖体。  |  Utley, RT., et al. 1998. Nature. 394: 498-502. PMID: 9697775
  12. 组蛋白乙酰转移酶和去乙酰化酶在基因调控中的作用。  |  Kuo, MH. and Allis, CD. 1998. Bioessays. 20: 615-26. PMID: 9780836

订购信息

产品名称产品编号规格价格数量收藏夹

Histone Deacetylase 4 抗体 (A-4)

sc-46672
200 µg/ml
$316.00

Histone Deacetylase 4 (HDAC4) (A-4): m-IgG Fc BP-HRP 套装

sc-528497
200 µg Ab; 10 µg BP
$354.00

Histone Deacetylase 4 (HDAC4) (A-4): m-IgGκ BP-HRP 套装

sc-520889
200 µg Ab, 40 µg BP
$354.00

Histone Deacetylase 4 (HDAC4) (A-4): m-IgG2b BP-HRP 套装

sc-550045
200 µg Ab; 10 µg BP
$354.00

Histone Deacetylase 4 (HDAC4) 抗体 (A-4) X

sc-46672 X
200 µg/0.1 ml
$316.00

Histone Deacetylase 4 (HDAC4) 抗体 (A-4) AC

sc-46672 AC
500 µg/ml, 25% agarose
$416.00

Histone Deacetylase 4 (HDAC4) 抗体 (A-4) HRP

sc-46672 HRP
200 µg/ml
$316.00

Histone Deacetylase 4 (HDAC4) 抗体 (A-4) FITC

sc-46672 FITC
200 µg/ml
$330.00

Histone Deacetylase 4 (HDAC4) 抗体 (A-4) PE

sc-46672 PE
200 µg/ml
$343.00

Histone Deacetylase 4 (HDAC4) 抗体 (A-4) Alexa Fluor® 488

sc-46672 AF488
200 µg/ml
$357.00

Histone Deacetylase 4 (HDAC4) 抗体 (A-4) Alexa Fluor® 546

sc-46672 AF546
200 µg/ml
$357.00

Histone Deacetylase 4 (HDAC4) 抗体 (A-4) Alexa Fluor® 594

sc-46672 AF594
200 µg/ml
$357.00

Histone Deacetylase 4 (HDAC4) 抗体 (A-4) Alexa Fluor® 647

sc-46672 AF647
200 µg/ml
$357.00

Histone Deacetylase 4 (HDAC4) 抗体 (A-4) Alexa Fluor® 680

sc-46672 AF680
200 µg/ml
$357.00

Histone Deacetylase 4 (HDAC4) 抗体 (A-4) Alexa Fluor® 790

sc-46672 AF790
200 µg/ml
$357.00

When should the TransCruz reagent sc-46672 X be used instead of the standard form sc-46672?

Asked by: TinTin
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 2 out of 5 by from Needs ultra ECL, also detects a smaller 60 kd bandUsing human blood monocyte derived macrophages, 25 ug total cell lysate ran on midi gels, 1:500 dilution in milk, 1:4000 2nd Ab CST anti-mouse we could detect a band at right size using Ultra lightning ECL. But there is also a smaller band ~60kda which is weaker.
Date published: 2021-07-19
Rated 3 out of 5 by from 抗体一般用了试用装，特异性还算比较强，但效价不高，需要上的浓度比较大，背景较为清晰，有需要应该会再次购买，谢谢
Date published: 2018-06-20
Rated 5 out of 5 by from Good quality！Work well in WB at 1:500.The band is quite clear.I incubated it at 4℃ for 14 hours.
Date published: 2017-12-13
Rated 5 out of 5 by from good quality!It work well in 1:500,transfer to the cellulose film took 1 and a half hours, the band is very clear.
Date published: 2017-12-13
Rated 5 out of 5 by from very goodWe bought this antibody last month, the concentration we used for WB is 1:500.
Date published: 2017-07-06
Rated 5 out of 5 by from Really strong antibody!Excellent choice for IHC on paraffin embedded tissue sections, and nice strong bands on Western blots. Solid antibody for HDAC4.
Date published: 2017-01-27
Rated 5 out of 5 by from Western blot and ChIP publication using T47DWestern blot and ChIP publication using T47D cell samples -SCBT Publication Review
Date published: 2015-03-05
Rated 5 out of 5 by from Publication using human thyroid paraffinPublication using human thyroid paraffin-embedded tissue samples in IHC -SCBT Publication Review
Date published: 2015-02-19
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Histone Deacetylase 4 (HDAC4) 抗体 (A-4) is rated 4.5 out of 5 by 11.
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