Date published: 2025-9-7
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Histone Deacetylase 3 (HDAC3) Antibody (A-3) - Western Blotting - Image 360945
HDAC3 抗体 (A-3): sc-376957. Jurkat (A), AN3 CA (B), NIH/3T3 (C), C6 (D), 3T3-L1 (E) 和 Rat2 (F) 全细胞裂解液中 HDAC3 表达的 Western 印迹分析.
HDAC3 抗体 (A-3): sc-376957. Jurkat (A), AN3 CA (B), NIH/3T3 (C), C6 (D), 3T3-L1 (E) 和 Rat2 (F) 全细胞裂解液中 HDAC3 表达的 Western 印迹分析.

Histone Deacetylase 3 (HDAC3) 抗体 (A-3): sc-376957

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寫評論提問

说明书
  • Histone Deacetylase 3 (HDAC3) 抗体 A-3 是小鼠单克隆 IgG1 κ，Histone Deacetylase 3 (HDAC3)抗体, 在33篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的HDAC3的N-terminus的氨基酸2-29之间
  • 建议使用Histone Deacetylase 3 (HDAC3)抗体（A-3）通过WB, IP, IF和ELISA检测HDAC3的mouse, rat, human和来源; 也和以下物种反应，包括： 和 equine, canine, bovine, porcine and avian
  • 抗Histone Deacetylase 3 (HDAC3)抗体（A-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-376957 X, 200 µg/0.1 ml)
  • 关于如何获取Histone Deacetylase 3 (HDAC3) (A-3): sc-376957的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是Histone Deacetylase 3 (HDAC3) Antibody (A-3) for WB applications. 的首选辅助检测试剂，这些试剂现与Histone Deacetylase 3 (HDAC3) Antibody (A-3) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外HDAC抗体，包括HDAC1, HDAC10, HDAC11, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8和HDAC9

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Histone Deacetylase 3 (HDAC3) 抗体 (A-3) 参考文献:

  1. 通过泛素化和降解 PML-RARα，靶向 HDAC3 克服急性早幼粒细胞白血病对 ATRA 或砷的耐药性。  |  Dai, B., et al. 2023. Cell Death Differ. 30: 1320-1333. PMID: 36894687
  2. HDAC3抑制通过增强CXCL10介导的趋化和免疫细胞的募集来促进抗肿瘤免疫。  |  Li, L., et al. 2023. Cancer Immunol Res. 11: 657-673. PMID: 36898011
  3. 巨噬细胞HDAC3抑制通过GBP5-NLRP3途径改善硫酸葡聚糖钠诱导的炎症性肠病。  |  Che, N., et al. 2024. Int J Med Sci. 21: 1385-1398. PMID: 38903915
  4. 乙酰化导致的核小体结构变化  |  Bauer, WR., et al. 1994. J Mol Biol. 236: 685-90. PMID: 8114086
  5. 组蛋白乙酰化在转录因子进入核糖体 DNA 的过程中发挥了积极作用。  |  Lee, DY., et al. 1993. Cell. 72: 73-84. PMID: 8422685
  6. 酵母的转录沉默与核小体乙酰化减少有关。  |  Braunstein, M., et al. 1993. Genes Dev. 7: 592-604. PMID: 8458576
  7. 四膜虫组蛋白乙酰转移酶 A：酵母 Gcn5p 的同源物，将组蛋白乙酰化与基因激活联系起来。  |  Brownell, JE., et al. 1996. Cell. 84: 843-51. PMID: 8601308
  8. 一种与酵母转录调节因子 Rpd3p 相关的哺乳动物组蛋白去乙酰化酶。  |  Taunton, J., et al. 1996. Science. 272: 408-11. PMID: 8602529
  9. 一种与腺病毒癌蛋白 E1A 竞争的 p300/CBP 相关因子。  |  Yang, XJ., et al. 1996. Nature. 382: 319-24. PMID: 8684459
  10. YY1 对转录的抑制是通过与哺乳动物中酵母全局调节因子 RPD3 的同源物相互作用来介导的。  |  Yang, WM., et al. 1996. Proc Natl Acad Sci U S A. 93: 12845-50. PMID: 8917507
  11. 将小鼠组蛋白去乙酰化酶 1 鉴定为生长因子诱导基因  |  Bartl, S., et al. 1997. Mol Cell Biol. 17: 5033-43. PMID: 9271381
  12. 人类 RPD3 同源物 HDAC3 的特征。  |  Emiliani, S., et al. 1998. Proc Natl Acad Sci U S A. 95: 2795-800. PMID: 9501169

订购信息

产品名称产品编号规格价格数量收藏夹

Histone Deacetylase 3 (HDAC3) 抗体 (A-3)

sc-376957
200 µg/ml
RMB2377.00

Histone Deacetylase 3 (HDAC3) (A-3): m-IgG Fc BP-HRP 套装

sc-527560
200 µg Ab; 10 µg BP
RMB2662.00

Histone Deacetylase 3 (HDAC3) (A-3): m-IgG1 BP-HRP 套装

sc-532933
200 µg Ab; 20 µg BP
RMB2662.00

Histone Deacetylase 3 (HDAC3) 抗体 (A-3) X

sc-376957 X
200 µg/0.1 ml
RMB2377.00

Histone Deacetylase 3 (HDAC3) 抗体 (A-3) AC

sc-376957 AC
500 µg/ml, 25% agarose
RMB3129.00

Histone Deacetylase 3 (HDAC3) 抗体 (A-3) HRP

sc-376957 HRP
200 µg/ml
RMB2377.00

Histone Deacetylase 3 (HDAC3) 抗体 (A-3) FITC

sc-376957 FITC
200 µg/ml
RMB2482.00

Histone Deacetylase 3 (HDAC3) 抗体 (A-3) PE

sc-376957 PE
200 µg/ml
RMB2580.00

Histone Deacetylase 3 (HDAC3) 抗体 (A-3) Alexa Fluor® 488

sc-376957 AF488
200 µg/ml
RMB2685.00

Histone Deacetylase 3 (HDAC3) 抗体 (A-3) Alexa Fluor® 546

sc-376957 AF546
200 µg/ml
RMB2685.00

Histone Deacetylase 3 (HDAC3) 抗体 (A-3) Alexa Fluor® 594

sc-376957 AF594
200 µg/ml
RMB2685.00

Histone Deacetylase 3 (HDAC3) 抗体 (A-3) Alexa Fluor® 647

sc-376957 AF647
200 µg/ml
RMB2685.00

Histone Deacetylase 3 (HDAC3) 抗体 (A-3) Alexa Fluor® 680

sc-376957 AF680
200 µg/ml
RMB2685.00

Histone Deacetylase 3 (HDAC3) 抗体 (A-3) Alexa Fluor® 790

sc-376957 AF790
200 µg/ml
RMB2685.00

Histone Deacetylase 3 (HDAC3) (A-3) 中和勝肽

sc-376957 P
100 µg/0.5 ml
RMB511.00

What is the recommended fixation method for IF with HDAC3 (A-3): sc-376957 monoclonal antibody?

Asked by: jenniferc
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10ºC methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support 5
Date published: 2024-06-23
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Rated 4 out of 5 by from b3 works wellUsed a 1:300 concentration on nuclear mouse brain lysate for chemiluminescence Western Blot, it would be better if the concentration was higher than 1:300
Date published: 2018-04-23
Rated 5 out of 5 by from wonderful antibody!1:200 diluted for wb of rat protein,the band is strong and special
Date published: 2018-04-21
Rated 5 out of 5 by from Works well!Band strong, specific and clear.........................................................................................
Date published: 2018-03-13
Rated 5 out of 5 by from It works well.Thanks for the trial sample. It works well.I'd like to recommend it.
Date published: 2017-11-27
Rated 4 out of 5 by from Good for western blot analysis of HDAC3 proteinAll lanes:HDAC3 抗体 (A-3): sc-376957 at 0.5ug/ml Lane1:SK-Hep1; Lane2:U2OS Lysates/protein at 80,000 cells per lane. I also used the antibody for immunofluorescence in SK-Hep1,but it doesn't work！
Date published: 2017-09-26
Rated 5 out of 5 by from good quality for WBThis antibody works well and generates a clear signal at the predicted position on the western blot (1:500 dilution, 50ug of rat heart extract).
Date published: 2017-09-21
Rated 5 out of 5 by from western bloti got this sample , work very well western blot with 3T3 -l1 cell lysis.
Date published: 2017-08-14
Rated 5 out of 5 by from Work well for WBGreat for WB, strong and clear band detected in 3T3L1 cell whole cell lysate.
Date published: 2017-08-09
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Histone Deacetylase 3 (HDAC3) 抗体 (A-3) is rated 4.8 out of 5 by 13.
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