Date published: 2025-9-7
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Histone Deacetylase 1 (HDAC1) Antibody (10E2) - Western Blotting - Image 372643
HDAC1 抗体 (10E2): sc-81598. NIH/3T3 (A), HeLa (B), K-562 (C), KNRK (D) 和 C32 (E) 核提取物中 HDAC1 表达的近红外 western 印迹分析. 使用 UltraCruz® 阻断试剂: sc-516214. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.
HDAC1 抗体 (10E2): sc-81598. NIH/3T3 (A), HeLa (B), K-562 (C), KNRK (D) 和 C32 (E) 核提取物中 HDAC1 表达的近红外 western 印迹分析. 使用 UltraCruz® 阻断试剂: sc-516214. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.

Histone Deacetylase 1 (HDAC1) 抗体 (10E2): sc-81598

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说明书
  • Histone Deacetylase 1 (HDAC1) 抗体 10E2 是小鼠单克隆 IgG1 κ，Histone Deacetylase 1 (HDAC1)抗体, 在161篇文献中引用，规格为200 µg/ml
  • 免疫human物种的HDAC1的氨基酸467-482a
  • Histone Deacetylase 1 (HDAC1) 抗体 (10E2) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 HDAC1; 不会与 other HDAC proteins产生交联反应
  • 抗Histone Deacetylase 1 (HDAC1)抗体（10E2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-81598 X, 200 µg/0.1 ml)
  • 关于如何获取Histone Deacetylase 1 (HDAC1) (10E2): sc-81598的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 Histone Deacetylase 1 (HDAC1) 抗体 (10E2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Histone Deacetylase 1 (HDAC1) 抗体 (10E2) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 HDAC 抗体 包括 HDAC1, HDAC10, HDAC11, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7和HDAC8

HDAC1抗体（10E2）是一种小鼠单克隆IgG1 kappa轻链抗体，通过识别人类蛋白中包含氨基酸467-482a的C端表位来靶向组蛋白去乙酰化酶1（HDAC1）。抗HDAC1抗体（10E2）对多种物种均具有强大的反应性，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）有效检测小鼠、大鼠和人类样本中的HDAC1。HDAC1，也称为GON-10、HD1、KDAC1、RPD3和RPD3L1，主要定位于细胞核内，通过脱乙酰化组蛋白蛋白在基因表达调控中发挥关键作用。这种脱乙酰化作用会导致染色质压缩和转录抑制，是维持基因组稳定性、控制细胞周期进展和促进细胞分化的关键过程。HDAC1在细胞核中的存在对于调节转录因子与DNA的接触至关重要，从而影响各种细胞通路和过程。HDAC1的失调与多种疾病有关，包括癌症、神经系统疾病和炎症，这使得HDAC1成为治疗干预的重要靶标和表观遗传学研究的关键焦点。HDAC1 (10E2) 抗体有非结合型和多种结合型变体，如琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和各种 Alexa Fluor® 结合物，可满足各种实验需求。抗HDAC1抗体（10E2）是研究人员探索表观遗传调控的复杂机制及其对细胞功能和疾病进展的影响时不可或缺的工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Histone Deacetylase 1 (HDAC1) 抗体 (10E2) 参考文献:

  1. HDAC1 通过调控 lncRNA/Slug 轴促进人类骨髓瘤细胞的迁移。  |  Zheng, L., et al. 2022. Int J Mol Med. 49: PMID: 34738621
  2. HDAC10通过去乙酰化STAT3来指导巨噬细胞M2程序，并促进过敏性气道炎症。  |  Chen, Y., et al. 2023. Biochem Cell Biol. 101: 52-63. PMID: 36542845
  3. 组蛋白去乙酰化酶HDAC1控制树突状细胞发育和抗肿瘤免疫。  |  Zhu, X., et al. 2024. Ocul Surf. 33: 39-49. PMID: 38679196
  4. METTL3-IGF2BP2促进HDAC1的过度表达，抑制代谢综合征相关性肝损伤中的FGF21表达。  |  De Sá Fernandes, C., et al. 2024. Cell Rep. 43: 114308. PMID: 38829740
  5. 乙酰化导致的核小体结构变化  |  Bauer, WR., et al. 1994. J Mol Biol. 236: 685-90. PMID: 8114086
  6. 组蛋白乙酰化在转录因子进入核糖体 DNA 的过程中发挥了积极作用。  |  Lee, DY., et al. 1993. Cell. 72: 73-84. PMID: 8422685
  7. 酵母的转录沉默与核小体乙酰化减少有关。  |  Braunstein, M., et al. 1993. Genes Dev. 7: 592-604. PMID: 8458576
  8. 四膜虫组蛋白乙酰转移酶 A：酵母 Gcn5p 的同源物，将组蛋白乙酰化与基因激活联系起来。  |  Brownell, JE., et al. 1996. Cell. 84: 843-51. PMID: 8601308
  9. 一种与酵母转录调节因子 Rpd3p 相关的哺乳动物组蛋白去乙酰化酶。  |  Taunton, J., et al. 1996. Science. 272: 408-11. PMID: 8602529
  10. 一种与腺病毒癌蛋白 E1A 竞争的 p300/CBP 相关因子。  |  Yang, XJ., et al. 1996. Nature. 382: 319-24. PMID: 8684459
  11. YY1 对转录的抑制是通过与哺乳动物中酵母全局调节因子 RPD3 的同源物相互作用来介导的。  |  Yang, WM., et al. 1996. Proc Natl Acad Sci U S A. 93: 12845-50. PMID: 8917507
  12. 将小鼠组蛋白去乙酰化酶 1 鉴定为生长因子诱导基因  |  Bartl, S., et al. 1997. Mol Cell Biol. 17: 5033-43. PMID: 9271381

订购信息

产品名称产品编号规格价格数量收藏夹

Histone Deacetylase 1 抗体 (10E2)

sc-81598
200 µg/ml
RMB2377.00

Histone Deacetylase 1 (HDAC1) (10E2): m-IgG Fc BP-HRP 套装

sc-528704
200 µg Ab; 10 µg BP
RMB2662.00

Histone Deacetylase 1 (HDAC1) (10E2): m-IgGκ BP-HRP 套装

sc-521172
200 µg Ab, 40 µg BP
RMB2662.00

Histone Deacetylase 1 (HDAC1) (10E2): m-IgG1 BP-HRP 套装

sc-543075
200 µg Ab; 20 µg BP
RMB2662.00

Histone Deacetylase 1 (HDAC1) 抗体 (10E2) X

sc-81598 X
200 µg/0.1 ml
RMB2377.00

Histone Deacetylase 1 (HDAC1) 抗体 (10E2) AC

sc-81598 AC
500 µg/ml, 25% agarose
RMB3129.00

Histone Deacetylase 1 (HDAC1) 抗体 (10E2) HRP

sc-81598 HRP
200 µg/ml
RMB2377.00

Histone Deacetylase 1 (HDAC1) 抗体 (10E2) FITC

sc-81598 FITC
200 µg/ml
RMB2482.00

Histone Deacetylase 1 (HDAC1) 抗体 (10E2) PE

sc-81598 PE
200 µg/ml
RMB2580.00

Histone Deacetylase 1 (HDAC1) 抗体 (10E2) Alexa Fluor® 488

sc-81598 AF488
200 µg/ml
RMB2685.00

Histone Deacetylase 1 (HDAC1) 抗体 (10E2) Alexa Fluor® 546

sc-81598 AF546
200 µg/ml
RMB2685.00

Histone Deacetylase 1 (HDAC1) 抗体 (10E2) Alexa Fluor® 594

sc-81598 AF594
200 µg/ml
RMB2685.00

Histone Deacetylase 1 (HDAC1) 抗体 (10E2) Alexa Fluor® 647

sc-81598 AF647
200 µg/ml
RMB2685.00

Histone Deacetylase 1 (HDAC1) 抗体 (10E2) Alexa Fluor® 680

sc-81598 AF680
200 µg/ml
RMB2685.00

Histone Deacetylase 1 (HDAC1) 抗体 (10E2) Alexa Fluor® 790

sc-81598 AF790
200 µg/ml
RMB2685.00

What is the recommended buffer for tis antibody? Is it PBST based or TBST based? Thank you.

Asked by: bam289
Thank you for your question. For most applications, we recommend using a TBST based buffer with this antibody. A link to a complete list of our recommended protocols is provided below as a reference. https://www.scbt.com/scbt/resources/protocols
Answered by: Technical Support
Date published: 2017-10-24

What is the recommended fixation method for IF with HDAC1 Antibody (10E2): sc-81598?

Asked by: jenni
We recommend fixing cells for 5 minutes in -10ºC methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support 12
Date published: 2017-01-09
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Rated 5 out of 5 by from 功能很出色！申请的小包装，说是50，实际就30不到，可能蒸发了。1：800稀释，检测肿瘤细胞中HDAC1的表达，条带清晰无背景，推荐！
Date published: 2025-02-15
Rated 5 out of 5 by from Histone Deacetylase 1 (HDAC1) 抗体效果很好。6月1日买的抗体，做了两次实验，就得到了很好的效果。santa cruz产品质量杠杠的，下次还会继续订购他们的产品
Date published: 2022-06-27
Rated 5 out of 5 by from Excellent in WBApplication: Western Blottiing Cell lysate: CFBE cells (human brochinal cells) Dilution: 1: 1000 Result: Clear and specific band at the expected molecular weight
Date published: 2021-12-27
Rated 5 out of 5 by from Really good antibodyReally good antibody for WB and IF applications. Used with nuclear extract and whole cell extract from human cell lines.
Date published: 2020-08-04
Rated 5 out of 5 by from HDAC1SINGLE BAND DETECTED WITH THE EXPECTED MOLECULAR MASS WITH AB DILUTION OF 1:5000
Date published: 2019-09-12
Rated 5 out of 5 by from Good response in WBThis product shows good reactivity with WB using HEK 293 lysate.
Date published: 2019-03-14
Rated 5 out of 5 by from Dr SadekAb work good with dilution 1:200 and 2 hrs incubation at 37ºc and overnight at 4 ºc
Date published: 2018-11-21
Rated 5 out of 5 by from Highly-recommended antibody for HDAC1I use this antibody to detect 3D pancreatic cancer cell line and cancer-related fibroblasts in WB and the results are very clear and clean. It could be used even at 1:5000 as attached! The green bands in the attached are detected by HDAC1 antibody (10E2) and the red ones are detected by SMA from another company.
Date published: 2018-04-27
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Histone Deacetylase 1 (HDAC1) 抗体 (10E2) is rated 4.9 out of 5 by 36.
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