Date published: 2025-9-6

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HB-EGF 抗体 (G-11): sc-74441

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说明书
  • HB-EGF 抗体 G-11 是小鼠单克隆 IgG2a κ,HB-EGF抗体, 在6篇文献中引用,规格为200 µg/ml
  • 免疫human物种的mature HB-EGF的氨基酸121-208
  • HB-EGF 抗体 (G-11) 推荐用于WB, IP, IF 和 ELISA,检测human 来源的 precursor and mature HB-EGF
  • 抗HB-EGF抗体(G-11)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取HB-EGF (G-11): sc-74441免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP2a BP-HRP">m-IgG2a BP-HRP是HB-EGF Antibody (G-11) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与HB-EGF Antibody (G-11) 打包提供(请参阅下面的订购信息)。

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関連項目

HB-EGF抗体(G-11)是一种小鼠单克隆IgG2a kappa轻链抗体,用于检测肝素结合EGF样生长因子(HB-EGF),也称为HBEGF或87个氨基酸的EGF样糖蛋白。HB-EGF单克隆抗体(G-11)靶向成熟人HB-EGF蛋白的121-208氨基酸,确保在蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和酶联免疫吸附(ELISA)检测中精确结合和可靠检测。HB-EGF是表皮生长因子(EGF)家族的重要成员,通过与EGF受体(EGFR)和细胞表面的硫酸肝素蛋白多糖结合,发挥强大的促分裂作用,对调节细胞增殖、分化及存活至关重要。HB-EGF基因编码一种208个氨基酸的前体,通过酶促裂解产生86个氨基酸的活性可溶性形式,突出了其在细胞信号传导过程中的关键作用。此外,HB-EGF的膜结合形式可作为白喉毒素受体,强调了其在生理和病理环境中的重要性。HB-EGF (G-11) 抗体有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 变体,为各种实验应用提供了多功能性。抗HB-EGF抗体(G-11)可识别人类样本中HB-EGF的前体和成熟形式,因此该抗体是研究生长因子信号通路和相关疾病机制的宝贵工具。HB-EGF(G-11)单克隆抗体有助于分子生物学和治疗开发方面的深入研究。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

HB-EGF 抗体 (G-11) 参考文献:

  1. 一种由巨噬细胞分泌的肝素结合型生长因子,与表皮生长因子有关。  |  Higashiyama, S., et al. 1991. Science. 251: 936-9. PMID: 1840698
  2. 山梨醇酯诱导细胞表面肝素结合型 EGF 样生长因子的快速处理:从并分泌型生长因子活性转化为旁分泌型生长因子活性。  |  Goishi, K., et al. 1995. Mol Biol Cell. 6: 967-80. PMID: 7579712
  3. 白喉毒素的内吞和膜转运依赖于完整的膜锚定受体(HB-EGF 前体):对经金属蛋白酶裂解的细胞相关受体在植物醇酯处理细胞中的研究。  |  Lanzrein, M., et al. 1995. Biochem J. 310 (Pt 1): 285-9. PMID: 7646457
  4. 膜锚定肝素结合型 EGF 样生长因子(HB-EGF)和白喉毒素受体相关蛋白(DRAP27)/CD9 在细胞-细胞接触点与整合素 alpha 3 beta 1 形成复合物。  |  Nakamura, K., et al. 1995. J Cell Biol. 129: 1691-705. PMID: 7790364
  5. 白喉毒素与人类肝素结合型 EGF 样生长因子/白喉毒素受体的表皮生长因子(EGF)样结构域结合,并特异性地抑制其有丝分裂活性。  |  Mitamura, T., et al. 1995. J Biol Chem. 270: 1015-9. PMID: 7836353
  6. 肝素结合表皮生长因子样生长因子 mRNA 在链脲佐菌素诱导的糖尿病大鼠肾脏中的表达增加。  |  Lee, YJ., et al. 1995. Biochem Biophys Res Commun. 207: 216-22. PMID: 7857268
  7. 作为 EGF 和 TGF alpha 拮抗物的 mAbs 可阻断 HB-EGF 与肿瘤细胞的结合。  |  Modjtahedi, H. and Dean, C. 1995. Biochem Biophys Res Commun. 207: 389-97. PMID: 7857293

订购信息

产品名称产品编号规格价格数量收藏夹

HB-EGF 抗体 (G-11)

sc-74441
200 µg/ml
RMB2377.00

HB-EGF (G-11): m-IgG Fc BP-HRP 套装

sc-525710
200 µg Ab; 10 µg BP
RMB2662.00

HB-EGF (G-11): m-IgG2a BP-HRP 套装

sc-546372
200 µg Ab; 10 µg BP
RMB2662.00

HB-EGF 抗体 (G-11) AC

sc-74441 AC
500 µg/ml, 25% agarose
RMB3129.00

HB-EGF 抗体 (G-11) HRP

sc-74441 HRP
200 µg/ml
RMB2377.00

HB-EGF 抗体 (G-11) FITC

sc-74441 FITC
200 µg/ml
RMB2482.00

HB-EGF 抗体 (G-11) PE

sc-74441 PE
200 µg/ml
RMB2580.00

HB-EGF 抗体 (G-11) Alexa Fluor® 488

sc-74441 AF488
200 µg/ml
RMB2685.00

HB-EGF 抗体 (G-11) Alexa Fluor® 546

sc-74441 AF546
200 µg/ml
RMB2685.00

HB-EGF 抗体 (G-11) Alexa Fluor® 594

sc-74441 AF594
200 µg/ml
RMB2685.00

HB-EGF 抗体 (G-11) Alexa Fluor® 647

sc-74441 AF647
200 µg/ml
RMB2685.00

HB-EGF 抗体 (G-11) Alexa Fluor® 680

sc-74441 AF680
200 µg/ml
RMB2685.00

HB-EGF 抗体 (G-11) Alexa Fluor® 790

sc-74441 AF790
200 µg/ml
RMB2685.00

I can't get the antibody to work on WB of cell lysate from Human and CHO cells-lines. I followed the protocols as cited unfortunately it is not working. Any recommendations, please?

Asked by: AmiraZine
Thank you for your question. We are sorry to hear that this antibody has not worked properly in your experiments yet. If you contact Technical Service directly with the parameters of your assay we will provide in-depth troubleshooting of your experiment.
Answered by: Tech Support Europe
Date published: 2021-07-19

Could this HB-EGF Antibody (G-11): sc-74441 be used to detect cytoplasmic tail fragment form of HB-EGF?

Asked by: XIERH
Thank you for your question. This antibody was raised against amino acids 121-208 of mature HB-EGF of human origin. The exact binding epitope has not been mapped. If you have any further questions, please contact your local distributor or Technical service directly.
Answered by: Tech Service
Date published: 2019-08-06

Could this HB-EGF Antibody (G-11): sc-74441 be used for mouse cells western blot? Could this antibody detect both precursor and mature form of HB-EGF, eliciting multiple target bands for mouse cells western blot?

Asked by: dentalong
Thank you for your question. This HB-EGF antibody was made against the mature form, but can detect both the precursor and mature form of HB-EGF. It is only recommended for human samples, however we do have a citation using it on mouse samples (PMID: 24244689). Please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Tech Service
Date published: 2017-09-05

Can HB-EGF (G-11): sc-74441 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: TinTin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, HB-EGF (G-11): sc-74441 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from too many bands when detect 293T cell lysateToo many bands in 293T cells makes you not sure which one is specific band.
Date published: 2017-10-10
Rated 5 out of 5 by from specific bandspecific band obtained 22kda,I suggest 1:400 diluted when analysis Human liver tissue cell.
Date published: 2017-06-24
Rated 5 out of 5 by from results is goodOutstanding results in western blotting, the background is very clean
Date published: 2017-06-01
Rated 5 out of 5 by from Wonderful results in our Western using thisWonderful results in our Western using this monoclonal.
Date published: 2015-09-11
Rated 5 out of 5 by from Nice Western blot data of human recombinant HBNice Western blot data of human recombinant HB-EGF protein. -SCBT QC
Date published: 2014-01-25
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HB-EGF 抗体 (G-11) is rated 4.6 out of 5 by 5.
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