Date published: 2025-9-8

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granulysin 抗体 (F-9): sc-271119

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说明书
  • granulysin 抗体 F-9 是小鼠单克隆 IgG2a κ,granulysin抗体, 在7篇文献中引用,规格为200 µg/ml
  • 针对氨基酸16-145,代表全长成熟mature granulysin,源自human
  • granulysin 抗体 (F-9) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测human 来源的 precursor and mature forms of granulysin
  • 抗granulysin抗体(F-9)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取granulysin (F-9): sc-271119免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG2a BP-HRP 是 granulysin 抗体 (F-9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与granulysin 抗体 (F-9) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

颗粒溶素抗体(F-9)是一种IgG2a κ小鼠单克隆颗粒溶素抗体(也称为颗粒溶素抗体),可通过WB、IP、IF、IHC(P)和ELISA检测人源性颗粒溶素蛋白。颗粒溶素抗体(F-9)既可以是非偶联的抗颗粒溶素抗体形式,也可以是多种偶联的抗颗粒溶素抗体形式,包括琼脂糖,HRP, PE, FITC和多个Alexa Fluor®偶联物。颗粒溶素(NKG5)是一种saposin样蛋白(SAPLIP),在颗粒细胞中表达,并作为造血系统中抗微生物反应的效应分子发挥作用。在细菌、真菌和寄生虫等细胞内病原体产生的抗原刺激下,颗粒溶素介导细胞毒性T淋巴细胞(ctl)和自然杀伤细胞(NKs)释放细胞毒性颗粒,这一过程可导致细胞凋亡。颗粒溶素的强效抗菌活性可能通过提高病原体宿主细胞通透性和溶解作用实现。人颗粒溶素表达为来源于三种独特转录本的两种大小的蛋白质。在穿孔素存在的情况下,表达ctl的颗粒溶素可通过颗粒依赖机制杀灭细胞内外结核分枝杆菌,提示颗粒溶素可能在T细胞介导的宿主抗某些病原体的保护中发挥广泛作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

granulysin 抗体 (F-9) 参考文献:

  1. 新型细胞溶解分子 granulysin 的生物合成。  |  Hanson, DA., et al. 1999. Mol Immunol. 36: 413-22. PMID: 10449094
  2. Granulysin: 细胞溶解 T 细胞的致命武器。  |  Stenger, S., et al. 1999. Immunol Today. 20: 390-4. PMID: 10462738
  3. 颗粒酶的表达随着牛皮癣临床严重程度的增加而增加。  |  Elgarhy, LH., et al. 2015. Clin Exp Dermatol. 40: 361-6. PMID: 25639185
  4. 死亡配体和粒细胞素:肿瘤细胞死亡诱导机制和治疗机会。  |  Martinez-Lostao, L., et al. 2015. Immunotherapy. 7: 883-2. PMID: 26314314
  5. 鼻型结节外 NK/T 细胞淋巴瘤的新型标记物 Granulysin。  |  Lo Bello, G., et al. 2018. Virchows Arch. 473: 749-757. PMID: 30151671
  6. 蜕膜NK细胞转移颗粒酶,选择性杀死滋养层细胞中的细菌。  |  Crespo, ÂC., et al. 2020. Cell. 182: 1125-1139.e18. PMID: 32822574
  7. 使用人类粒细胞素转基因小鼠评估CD8 T细胞对结核分枝杆菌免疫中的粒细胞素表达作用。  |  Thakur, P., et al. 2022. mBio. 13: e0302022. PMID: 36409085
  8. M1激活的巨噬细胞和表达穿孔素/粒酶的淋巴细胞在IgA血管炎肾炎中的参与。  |  Laskarin, G., et al. 2024. Int J Mol Sci. 25: PMID: 38396930
  9. 人类 NK 和 T 细胞特异性激活基因 NKG5 的基因组结构。  |  Houchins, JP., et al. 1993. Immunogenetics. 37: 102-7. PMID: 8423048
  10. 一种可能参与细胞介导的细胞毒性的新型人类细胞溶解颗粒缔合作用蛋白--Granulysin。  |  Peña, SV. and Krensky, AM. 1997. Semin Immunol. 9: 117-25. PMID: 9194222
  11. Granulysin 诱导的细胞凋亡。I. 至少两种不同途径的参与。  |  Gamen, S., et al. 1998. J Immunol. 161: 1758-64. PMID: 9712041
  12. 由粒细胞溶素介导的细胞溶解 T 细胞的抗菌活性。  |  Stenger, S., et al. 1998. Science. 282: 121-5. PMID: 9756476

订购信息

产品名称产品编号规格价格数量收藏夹

granulysin 抗体 (F-9)

sc-271119
200 µg/ml
RMB2377.00

granulysin (F-9): m-IgG Fc BP-HRP 套装

sc-525784
200 µg Ab; 10 µg BP
RMB2662.00

granulysin (F-9): m-IgG2a BP-HRP 套装

sc-546414
200 µg Ab; 10 µg BP
RMB2662.00

granulysin 抗体 (F-9) AC

sc-271119 AC
500 µg/ml, 25% agarose
RMB3129.00

granulysin 抗体 (F-9) HRP

sc-271119 HRP
200 µg/ml
RMB2377.00

granulysin 抗体 (F-9) FITC

sc-271119 FITC
200 µg/ml
RMB2482.00

granulysin 抗体 (F-9) PE

sc-271119 PE
200 µg/ml
RMB2580.00

granulysin 抗体 (F-9) Alexa Fluor® 488

sc-271119 AF488
200 µg/ml
RMB2685.00

granulysin 抗体 (F-9) Alexa Fluor® 546

sc-271119 AF546
200 µg/ml
RMB2685.00

granulysin 抗体 (F-9) Alexa Fluor® 594

sc-271119 AF594
200 µg/ml
RMB2685.00

granulysin 抗体 (F-9) Alexa Fluor® 647

sc-271119 AF647
200 µg/ml
RMB2685.00

granulysin 抗体 (F-9) Alexa Fluor® 680

sc-271119 AF680
200 µg/ml
RMB2685.00

granulysin 抗体 (F-9) Alexa Fluor® 790

sc-271119 AF790
200 µg/ml
RMB2685.00

Does this antibody require any sort of HIER or enzyme epitope retrieval? Thank you!

Asked by: Betsy
Thank you for your question. Yes, we do recommend antigen retrieval. Heat treatment (recommended method): Place slides in a container and cover with 10 mM sodium citrate buffer, pH 6.0 (sc-294091); or with 50 mM glycine-HCl buffer (sc-29096), pH 3.5, with 0.01% (w/v) EDTA (sc-29092). Heat at 95° C for 5 minutes. Top off with fresh buffer and heat at 95° C for 5 minutes (optimal incubation time may vary for each tissue type). Allow slides to cool in the buffer for approximately 20 minutes. Wash in deionized H2O three times for 2 minutes each. Aspirate excess liquid from slides.
Answered by: BlakeJ
Date published: 2024-03-28

Hi, does this Ab recognise both forms (9 and 15 kDa) of Gnly? Thanks.

Asked by: B77w81
Thank you for your question. Yes, this antibody can detect both precursor and mature forms of granulysin.
Answered by: Tech Service
Date published: 2024-07-22

I am using granulysin (F-9): sc-271119 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: DefinitelyNotMatt
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
  • y_2025, m_8, d_30, h_9CST
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Rated 5 out of 5 by from perfectwe used this antibody for WB on blood samples, it works very well
Date published: 2017-10-23
Rated 5 out of 5 by from Nice antiboy dor granulysin detectionWe used this antibody in a western blot experiment for granulysin expression and got a dark band at the correct molecular weight
Date published: 2017-01-30
Rated 5 out of 5 by from Produced superb immunoperoxidase cytoplasmicProduced superb immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human lymph node tissue. -SCBT QC
Date published: 2015-02-27
Rated 5 out of 5 by from Produced positive Western Blot data of granulysinProduced positive Western Blot data of granulysin expression in NK-92 whole cell lysate. -SCBT QC
Date published: 2013-05-10
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granulysin 抗体 (F-9) is rated 5.0 out of 5 by 4.
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