Date published: 2025-9-7

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GPI 抗体 (H-10): sc-365066

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说明书
  • GPI 抗体 H-10 是小鼠单克隆 IgG3 κ,GPI抗体, 在7篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的GPI的N-terminus附近的氨基酸67-102之间
  • 推荐用于 mouse, rat 和 human 来源的GPI WB, IP, IF 和 ELISA检测; 也和以下物种反应,包括: porcine
  • 关于如何获取GPI (H-10): sc-365066免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG3 BP-HRP是GPI Antibody (H-10) 适用于 WB 应用。 的首选辅助检测试剂,该试剂现与GPI Antibody (H-10) 搭配使用(请参阅下面的订购信息)。

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関連項目

GPI抗体(H-10)是一种IgG3 κ小鼠单克隆GPI抗体,可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的GPI蛋白。GPI抗体(H-10)可以是非偶联的抗GPI抗体。葡萄糖-6-磷酸异构酶(GPI)有许多其他名称,包括磷酸己糖异构酶(PHI)、神经白介素(NLK)和精子抗原-36(SA-36)。GPI是一种属于GPI家族的胞质同源二聚体。它是脊髓和感觉神经元的神经营养因子,参与糖酵解和糖异生。GPI的缺陷或突变可引起遗传性非球形溶血性贫血(HA)、胎儿水肿、新生儿立即死亡和神经系统损伤。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

GPI 抗体 (H-10) 参考文献:

  1. 葡萄糖-6-磷酸异构酶不是类风湿性关节炎中特异性的自身抗原。  |  Herve, CA., et al. 2003. Rheumatology (Oxford). 42: 986-8. PMID: 12730513
  2. 葡萄糖-6-磷酸异构酶是家养雪貂胚胎植入所必需的。  |  Schulz, LC. and Bahr, JM. 2003. Proc Natl Acad Sci U S A. 100: 8561-6. PMID: 12826606
  3. 使用表面等离子共振(BIAcore)在类风湿性关节炎患者的滑液中检测抗葡萄糖-6-磷酸异构酶的抗体。  |  Kim, JY., et al. 2003. Exp Mol Med. 35: 310-6. PMID: 14508072
  4. 葡萄糖-6-磷酸异构酶免疫可诱导基因未改变的小鼠患上T细胞依赖性外周多关节炎。  |  Schubert, D., et al. 2004. J Immunol. 172: 4503-9. PMID: 15034067
  5. 分辨率为 1.6A 的小鼠磷酸葡萄糖异构酶晶体结构及其与 6-磷酸葡萄糖的复合物揭示了糖环打开的催化机制。  |  Solomons, JT., et al. 2004. J Mol Biol. 342: 847-60. PMID: 15342241
  6. 葡萄糖-6-磷酸异构酶变体在抗 GPI 抗体的生成中起关键作用:自身抗体生成的可能机制。  |  Muraki, Y., et al. 2004. Biochem Biophys Res Commun. 323: 518-22. PMID: 15369782
  7. 炎症性关节炎患者血清和滑液中抗葡萄糖-6-磷酸异构酶IgG水平升高。  |  Schaller, M., et al. 2005. Ann Rheum Dis. 64: 743-9. PMID: 15458959
  8. 类风湿性关节炎患者血清葡萄糖-6-磷酸异构酶水平升高与组织学疾病活动和治疗后临床改善相关。  |  Dai, L., et al. 2010. J Rheumatol. 37: 2452-61. PMID: 20810510
  9. 在葡萄糖-6-磷酸异构酶诱导的关节炎模型中,4型精氨酰精氨酸脱亚胺酶缺乏症减轻了关节炎严重程度。  |  Seri, Y., et al. 2015. Sci Rep. 5: 13041. PMID: 26293116
  10. 两种新的突变(p.(Ser160Pro)和p.(Arg472Cys))会导致葡萄糖-6-磷酸异构酶缺乏症,与红细胞发育不良和铁调素抑制不当有关。  |  Mojzikova, R., et al. 2018. Blood Cells Mol Dis. 69: 23-29. PMID: 28803808
  11. 葡萄糖磷酸异构酶(GPI)缺乏症突变与遗传性非非球形溶血性贫血(HNSHA)有关。  |  Beutler, E., et al. 1997. Blood Cells Mol Dis. 23: 402-9. PMID: 9446754
  12. 人类葡萄糖-6-磷酸异构酶(GPI)缺乏症神经功能障碍和溶血性贫血的分子基础。  |  Kugler, W., et al. 1998. Hum Genet. 103: 450-4. PMID: 9856489

订购信息

产品名称产品编号规格价格数量收藏夹

GPI 抗体 (H-10)

sc-365066
200 µg/ml
RMB2377.00

GPI (H-10): m-IgG3 BP-HRP 套装

sc-550565
200 µg Ab; 40 µg BP
RMB2662.00

GPI (H-10) 中和勝肽

sc-365066 P
100 µg/0.5 ml
RMB511.00

This product was recommended through the GPI anchor antibody page, but this is an antibody of GPI protein, not for GPI anchor. Which product is used for GPI anchored modification detecion?

Asked by: Jovian
Thank you for your question. The immunogen information of this antibody is: specific for an epitope mapping between amino acids 67-102 near the N-terminus of GPI of human origin. This primary antibody is recommended for detection of GPI of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including porcine.
Answered by: Ling Chen
Date published: 2025-03-24

What application is the blocking peptide sc-365066 P appropriate for?

Asked by: Randy McDonald
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from GoodWe checked GPI expression in mouse liver lysate, 50ug protein, 1:1000 primary antibody O/N, the band is clear and very specific.
Date published: 2017-07-20
Rated 5 out of 5 by from Produced superb immunofluorescence membraneProduced superb immunofluorescence membrane staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-02-06
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GPI 抗体 (H-10) is rated 5.0 out of 5 by 3.
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