Date published: 2025-9-7

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glypican-5 抗体 (F-3): sc-390838

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说明书
  • glypican-5 抗体 F-3 是小鼠单克隆 IgG1 (kappa轻链),glypican-5抗体,规格为200 µg/ml
  • 免疫原氨基酸序列301-460位于human物种的glypican-5的内部区域内
  • glypican-5 抗体 (F-3) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 glypican-5
  • 抗glypican-5抗体(F-3)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取glypican-5 (F-3): sc-390838免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是glypican-5 Antibody (F-3) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与glypican-5 Antibody (F-3) 打包提供(请参阅下面的订购信息)。

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    関連項目

    glypican-5抗体(F-3)是一种IgG1 κ小鼠单克隆glypican-5抗体(也被称为Glypican 5抗体或GPC5抗体),通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和酶联免疫吸附测定(ELISA)检测小鼠、大鼠和人类来源的glypican-5蛋白。glypican-5抗体(F-3)既有非偶联形式的抗glypican-5抗体,也有多种偶联形式的抗glypican-5抗体,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®偶联物。Glypicans被认为能够激活或控制形态发生因子和生长因子的活性,从而影响细胞分裂、细胞生长和分化的调控。神经元的生长和修复也被认为是由glypicans控制的。Glypican-5也被称为GPC5,是一种由572个氨基酸组成的蛋白质。Glypican-5主要在成人脑组织(定位于神经元)中表达,但在胎儿脑、肺和肝中也有发现。Glypican-5是glypican相关整体膜蛋白聚糖家族(GRIPS)的成员,其特点是具有一个能够利用糖基磷脂酰肌醇附着于细胞膜的核心蛋白。在多发性硬化症的活跃斑块中发现了glypican-5的上调,这表明glypican-5可能参与了多发性硬化症的发病机理。glypican-5的上调也被认为与淋巴瘤以及其他肿瘤有很强的相关性。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    glypican-5 抗体 (F-3) 参考文献:

    1. GPC6 是糖蛋白基因家族的新成员,它编码的产物在结构上与 GPC4 相关,并与 GPC5 共同定位于人类 13 号染色体上。  |  Paine-Saunders, S., et al. 1999. Genomics. 57: 455-8. PMID: 10329016
    2. 糖蛋白的发育作用。  |  De Cat, B. and David, G. 2001. Semin Cell Dev Biol. 12: 117-25. PMID: 11292377
    3. 染色体 13q32 上 GPC5/GPC6 基因簇的 4-Mb BAC/PAC contig 和完整基因组结构。  |  Veugelers, M., et al. 2001. Matrix Biol. 20: 375-85. PMID: 11566272
    4. GPC5 可能是淋巴瘤细胞系中检测到的 13q31-q32 扩增的靶点。  |  Yu, W., et al. 2003. J Hum Genet. 48: 331-335. PMID: 12721791
    5. 多发性硬化症易感性和临床表型的全基因组关联分析。  |  Baranzini, SE., et al. 2009. Hum Mol Genet. 18: 767-78. PMID: 19010793
    6. 12 名新的 13q 缺失综合征患者:正在进行基因型-表型分析。  |  Quélin, C., et al. 2009. Eur J Med Genet. 52: 41-6. PMID: 19022413
    7. 糖蛋白基因家族新成员--人 GPC5 的糖蛋白-5 的特征和染色体定域。  |  Veugelers, M., et al. 1997. Genomics. 40: 24-30. PMID: 9070915
    8. 细胞表面蛋白聚糖 glypican-5 在肾脏, 四肢和大脑中的表达受发育调控。  |  Saunders, S., et al. 1997. Dev Biol. 190: 78-93. PMID: 9331333

    订购信息

    产品名称产品编号规格价格数量收藏夹

    glypican-5 抗体 (F-3)

    sc-390838
    200 µg/ml
    RMB2377.00

    glypican-5 (F-3): m-IgG Fc BP-HRP 套装

    sc-530279
    200 µg Ab; 10 µg BP
    RMB2662.00

    glypican-5 (F-3): m-IgGκ BP-HRP 套装

    sc-523701
    200 µg Ab, 40 µg BP
    RMB2662.00

    glypican-5 (F-3): m-IgG1 BP-HRP 套装

    sc-543970
    200 µg Ab; 20 µg BP
    RMB2662.00

    glypican-5 抗体 (F-3) AC

    sc-390838 AC
    500 µg/ml, 25% agarose
    RMB3129.00

    glypican-5 抗体 (F-3) HRP

    sc-390838 HRP
    200 µg/ml
    RMB2377.00

    glypican-5 抗体 (F-3) FITC

    sc-390838 FITC
    200 µg/ml
    RMB2482.00

    glypican-5 抗体 (F-3) PE

    sc-390838 PE
    200 µg/ml
    RMB2580.00

    glypican-5 抗体 (F-3) Alexa Fluor® 488

    sc-390838 AF488
    200 µg/ml
    RMB2685.00

    glypican-5 抗体 (F-3) Alexa Fluor® 546

    sc-390838 AF546
    200 µg/ml
    RMB2685.00

    glypican-5 抗体 (F-3) Alexa Fluor® 594

    sc-390838 AF594
    200 µg/ml
    RMB2685.00

    glypican-5 抗体 (F-3) Alexa Fluor® 647

    sc-390838 AF647
    200 µg/ml
    RMB2685.00

    glypican-5 抗体 (F-3) Alexa Fluor® 680

    sc-390838 AF680
    200 µg/ml
    RMB2685.00

    glypican-5 抗体 (F-3) Alexa Fluor® 790

    sc-390838 AF790
    200 µg/ml
    RMB2685.00

    What is the recommended dilution for IF for glypican-5 (F3) monoclonal antibody sc-390838? Which is also the recommended fixation method?

    Asked by: Federico
    Thank you for your question. The recommended starting dilution for this product for IF applications is 1:50. We recommend using acetone or methanol for fixation. Here is a link to the recommended protocol: https://www.scbt.com/scbt/resources/protocols If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com
    Answered by: Tech Service
    Date published: 2017-10-11

    What is the recommended starting dilution for Western blot for sc-390838: glypican-5 (F-3) monoclonal antibody?

    Asked by: Professor Griffin
    The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
    Answered by: Technical Support
    Date published: 2017-03-22
    • y_2025, m_9, d_7, h_4CST
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    Rated 5 out of 5 by from perfectI used this antibody for detection of glypican-5 of human origined cells by WB
    Date published: 2016-12-05
    Rated 4 out of 5 by from This antibody works well for detecting GPC5This antibody works well for detecting GPC5 by western blot in 293T transfected cells. We have tried several antibodies from other sources and most of them picked up some non-specific bands (293T cells un-transfected and transfected with GPC5 and other GPC family members). Don't know if it is sensitive enough to pick up endogenous GPC5.<br /> That being said, I think it is outrageous to present as reviews internal comments from the SCBT QC team. That is not a review, it should be part of what you do before releasing an antibody. A quick look at your website shows a lot of antibodies with 5 stars based on one "review" from the SCBT QC team. That is misleading and deceptive,<br /> Enough said.
    Date published: 2016-04-19
    Rated 5 out of 5 by from Great Western blot data of glypicanGreat Western blot data of glypican-5 expression in non-transfected and human glypican-5 transfected 293T whole cell lysates. -SCBT QC
    Date published: 2014-05-16
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    glypican-5 抗体 (F-3) is rated 4.7 out of 5 by 3.
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