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Glutathione reductase Antibody (B-12) - Western Blotting - Image 42493
谷胱甘肽还原酶抗体 (B-12): sc-133159. IMR-32 (A) 和 JEG-3 (B) 全细胞裂解液中谷胱甘肽还原酶表达的 Western 印迹分析.
谷胱甘肽还原酶抗体 (B-12): sc-133159. IMR-32 (A) 和 JEG-3 (B) 全细胞裂解液中谷胱甘肽还原酶表达的 Western 印迹分析.

Glutathione reductase 抗体 (B-12): sc-133159

4.3(4)
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说明书
  • Glutathione reductase抗体(B-12)是小鼠单克隆IgG2a κ, 在9篇文献中引用，规格为200 µg/ml
  • 免疫human来源Glutathione reductase近C-terminus的391-510氨基酸
  • 推荐用于 mouse, rat 和 human 来源的Glutathione reductase WB, IP, IF, IHC(P) 和 ELISA检测
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 Glutathione reductase 抗体 (B-12) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Glutathione reductase 抗体 (B-12) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

谷胱甘肽还原酶，也被称为谷胱甘肽还原酶线粒体前体、GRase、GSR或GR，属于I类吡啶核苷酸-二硫化物氧化还原酶家族。该蛋白质的主要功能是在细胞质中维持高水平的还原型谷胱甘肽。谷胱甘肽还原酶在NADPH氧化的同时，将氧化型谷胱甘肽转化为还原型。谷胱甘肽还原酶可以定位于线粒体或细胞质，并能形成二硫键连接的同源二聚体。该蛋白质的活性部位是一个具有氧化还原活性的二硫键。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Glutathione reductase 抗体 (B-12) 参考文献:

  1. 体外实验中，乙酰氨基酚-谷胱甘肽结合物抑制谷胱甘肽还原酶。  |  Rousar, T., et al. 2010. Physiol Res. 59: 225-232. PMID: 19537930
  2. 谷胱甘肽还原酶/谷胱甘肽通过真菌中的多硫穿梭负责细胞毒性元素硫的耐受性。  |  Sato, I., et al. 2011. J Biol Chem. 286: 20283-91. PMID: 21474441
  3. 谷胱甘肽还原酶介导的含碲纳米结构的合成表现出抗菌特性。  |  Pugin, B., et al. 2014. Appl Environ Microbiol. 80: 7061-70. PMID: 25193000
  4. 单脱氢抗坏血酸还原酶和谷胱甘肽还原酶对硝化和S-亚硝化作用的分子反应差异。  |  Begara-Morales, JC., et al. 2015. J Exp Bot. 66: 5983-96. PMID: 26116026
  5. 血浆谷胱甘肽还原酶活性与脓毒症休克的预后。  |  Kim, JS., et al. 2016. J Surg Res. 200: 298-307. PMID: 26316444
  6. 富马酸二甲酯通过上调谷胱甘肽还原酶诱导谷胱甘肽再循环  |  Hoffmann, C., et al. 2017. Oxid Med Cell Longev. 2017: 6093903. PMID: 28116039
  7. 血矛线虫中一种具有额外谷胱甘肽还原酶活性的新型硫氧化还原蛋白还原酶。  |  Plancarte, A., et al. 2017. Exp Parasitol. 177: 82-92. PMID: 28456691
  8. 谷胱甘肽还原酶通过调节氧化还原平衡介导胶质母细胞瘤细胞的耐药性。  |  Zhu, Z., et al. 2018. J Neurochem. 144: 93-104. PMID: 29105080
  9. 谷胱甘肽还原酶介导的硫醇氧化应激抑制了小鼠黑色素瘤细胞的转移。  |  Li, X., et al. 2018. Free Radic Biol Med. 129: 256-267. PMID: 30086340
  10. 谷胱甘肽还原酶的替代降低了现代人类的电子泄漏和炎症。  |  Coppo, L., et al. 2022. Sci Adv. 8: eabm1148. PMID: 34985944

订购信息

产品名称产品编号规格价格数量收藏夹

Glutathione reductase 抗体 (B-12)

sc-133159
200 µg/ml
RMB2377.00

Glutathione reductase (B-12): m-IgG Fc BP-HRP 套装

sc-537348
200 µg Ab; 10 µg BP
RMB2662.00

Glutathione reductase (B-12): m-IgGκ BP-HRP 套装

sc-534591
200 µg Ab; 40 µg BP
RMB2662.00

Glutathione reductase (B-12): m-IgG2a BP-HRP 套装

sc-547988
200 µg Ab; 10 µg BP
RMB2662.00

Can Glutathione reductase (B-12): sc-133159 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: AbPolly
Thank you for your question. Yes, Glutathione reductase (B-12): sc-133159 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 4 out of 5 by from Anti-GSR for western blottingGood inmunolabeling of GSR by western blotting, tested to check differences in antioxidant enzymes under different metabolic conditions and mitochondrial function.
Date published: 2023-04-10
Rated 4 out of 5 by from Various publishable data in WB using human HSB2Various publishable data in WB using human HSB2, HUVEC, HeLa, HSB2, Molt3 and CiHHV6A cells -SCBT Publication Review
Date published: 2015-07-05
Rated 5 out of 5 by from Produced superb immunofluorescence cytoplasmicProduced superb immunofluorescence cytoplasmic staining in methanol-fixed NIH/3T3 cells. -SCBT QC
Date published: 2015-03-25
Rated 4 out of 5 by from Publishable data in WB using mouse liverPublishable data in WB using mouse liver. -SCBT Publication Review
Date published: 2015-03-06
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Glutathione reductase 抗体 (B-12) is rated 4.3 out of 5 by 4.
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