Date published: 2025-9-11
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
Glucose Transporter Glut5 Antibody (E-2) - Western Blotting - Image 137735
Glut5 抗体 (E-2): sc-271055. 大鼠小肠组织提取物 (A) 和 HeLa (B), HEL 92. 1. 7 (C) 和 HEK293 (D) 全细胞裂解液中 Glut5 表达的 Western 印迹分析.
Glut5 抗体 (E-2): sc-271055. 大鼠小肠组织提取物 (A) 和 HeLa (B), HEL 92. 1. 7 (C) 和 HEK293 (D) 全细胞裂解液中 Glut5 表达的 Western 印迹分析.

Glucose Transporter Glut5 抗体 (E-2): sc-271055

4.8(9)
寫評論提問

说明书
  • Glucose Transporter Glut5 抗体 E-2 是小鼠单克隆 IgG1 κ，Glucose Transporter Glut5抗体, 在17篇文献中引用，规格为200 µg/ml
  • 针对氨基酸221-420的图谱，在Glut5的内部区域，human起源
  • Glucose Transporter Glut5 抗体 (E-2) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Glut5
  • 抗Glucose Transporter Glut5抗体（E-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Glucose Transporter Glut5 (E-2): sc-271055的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 Glucose Transporter Glut5 抗体 (E-2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Glucose Transporter Glut5 抗体 (E-2) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 Glut 抗体 包括 Glut1, Glut10, Glut2, Glut3, Glut4, Glut5和Glut6

Glut5抗体（E-2）是一种IgG1κ型小鼠单克隆Glut5抗体（也称为SLC2A5抗体），可以通过WB、IP、IF、IHC（P）和ELISA等方法检测到小鼠、大鼠和人类来源的Glut5蛋白。Glut5抗体（E-2）可以以非共轭型抗Glut5抗体形式获得，也可以以多种共轭型抗Glut5抗体形式获得，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。葡萄糖是我们能量的主要来源，在哺乳动物中，葡萄糖转运体有多种异构体，包括Glut1、Glut2、Glut3、Glut4、Glut5、Glut6、Glut7、Glut8和Glut9。位于人类染色体1短臂上的GLUT5基因编码一个含有501个氨基酸的易化型葡萄糖转运体。Glut5的mRNA在小肠中高度表达，在肾脏、骨骼肌和脂肪组织中的表达程度较低。Glut5在小肠果糖吸收中起关键作用，当暴露于富含果糖的饮食时，其表达会受到高度诱导。在人体骨骼肌中表达的Glut5转运体特异性地定位于质膜上，参与调节己糖在肌膜上的转运。Glut8是一种新型的葡萄糖转运体样蛋白，与其他糖转运体家族成员具有显著的序列相似性。Glut8包含12个假定的跨膜螺旋和几个保守的基序，这对转运活性非常重要。在人类组织中，Glut8主要在睾丸中表达，在包括骨骼肌、心脏、小肠和大脑在内的大多数其他组织中的表达程度较低。此外，Glut8葡萄糖转运促进剂还具有激素调节的睾丸功能。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Glucose Transporter Glut5 抗体 (E-2) 参考文献:

  1. 具有葡萄糖转运活性的糖转运促进因子家族新成员 GLUT8。  |  Doege, H., et al. 2000. J Biol Chem. 275: 16275-80. PMID: 10821868
  2. 人类骨骼肌中 'GLUT5 葡萄糖转运体 '的生物化学和免疫细胞化学定域。  |  Hundal, HS., et al. 1992. Biochem J. 286 (Pt 2): 339-43. PMID: 1530566
  3. 人类促进性葡萄糖转运体。编码一种在小肠, 肾脏, 肌肉和脂肪组织中表达的同工型（GLUT5）和一种不常见的葡萄糖转运体假基因样序列（GLUT6）的 cDNAs 的分离, 功能表征和基因定位。  |  Kayano, T., et al. 1990. J Biol Chem. 265: 13276-82. PMID: 1695905
  4. GLUT5（SLC2A5）能使果糖介导的增殖独立于酮六磷酸酶。  |  Liang, RJ., et al. 2021. Cancer Metab. 9: 12. PMID: 33762003
  5. GLUT5 是饮食果糖介导的实验性结肠炎恶化的决定因素。  |  Basu, S., et al. 2021. Am J Physiol Gastrointest Liver Physiol. 321: G232-G242. PMID: 34133236
  6. 敲除大鼠孤束核中的 Glut5 可缓解果糖诱发的高血压  |  Wu, CJ., et al. 2022. J Nutr. 152: 448-457. PMID: 34687200
  7. IL-6/STAT3 轴激活 Glut5 以调控果糖代谢和肿瘤发生  |  Huang, X., et al. 2022. Int J Biol Sci. 18: 3668-3675. PMID: 35813468
  8. 与 GLUT5 表达相关的致癌影响。  |  Hadzi-Petrushev, N., et al. 2024. Mol Med. 30: 114. PMID: 39107723
  9. 大鼠果糖转运体 GLUT5 的序列, 组织分布和功能特征。  |  Rand, EB., et al. 1993. Am J Physiol. 264: G1169-76. PMID: 8333543
  10. 大鼠空肠 GLUT5 的克隆和果糖喂养时的表达量增加。  |  Inukai, K., et al. 1993. Endocrinology. 133: 2009-14. PMID: 8404647
  11. 人体骨骼肌中 GLUT5 的表达和果糖转运  |  Hundal, HS., et al. 1998. Adv Exp Med Biol. 441: 35-45. PMID: 9781312
  12. 大鼠骨骼肌中 GLUT5 果糖转运体的生化和功能特征。  |  Darakhshan, F., et al. 1998. Biochem J. 336 (Pt 2): 361-6. PMID: 9820812

订购信息

产品名称产品编号规格价格数量收藏夹

Glucose Transporter Glut5 抗体 (E-2)

sc-271055
200 µg/ml
RMB2377.00

Glucose Transporter Glut5 (E-2): m-IgG Fc BP-HRP 套装

sc-529107
200 µg Ab; 10 µg BP
RMB2662.00

Glucose Transporter Glut5 (E-2): m-IgGκ BP-HRP 套装

sc-521831
200 µg Ab, 40 µg BP
RMB2662.00

Glucose Transporter Glut5 (E-2): m-IgG1 BP-HRP 套装

sc-543323
200 µg Ab; 20 µg BP
RMB2662.00

Glucose Transporter Glut5 抗体 (E-2) AC

sc-271055 AC
500 µg/ml, 25% agarose
RMB3129.00

Glucose Transporter Glut5 抗体 (E-2) HRP

sc-271055 HRP
200 µg/ml
RMB2377.00

Glucose Transporter Glut5 抗体 (E-2) FITC

sc-271055 FITC
200 µg/ml
RMB2482.00

Glucose Transporter Glut5 抗体 (E-2) PE

sc-271055 PE
200 µg/ml
RMB2580.00

Glucose Transporter Glut5 抗体 (E-2) Alexa Fluor® 488

sc-271055 AF488
200 µg/ml
RMB2685.00

Glucose Transporter Glut5 抗体 (E-2) Alexa Fluor® 546

sc-271055 AF546
200 µg/ml
RMB2685.00

Glucose Transporter Glut5 抗体 (E-2) Alexa Fluor® 594

sc-271055 AF594
200 µg/ml
RMB2685.00

Glucose Transporter Glut5 抗体 (E-2) Alexa Fluor® 647

sc-271055 AF647
200 µg/ml
RMB2685.00

Glucose Transporter Glut5 抗体 (E-2) Alexa Fluor® 680

sc-271055 AF680
200 µg/ml
RMB2685.00

Glucose Transporter Glut5 抗体 (E-2) Alexa Fluor® 790

sc-271055 AF790
200 µg/ml
RMB2685.00

Can you recommend an isotype control for this product?

Asked by: westernblotter
Thank you for your questions. We would recommend sc-3877 as an isotype control.
Answered by: Technical Service
Date published: 2023-10-10

Are these antibodies phosphor-specific? I would like to make sure that I reduce the chances that background proteins will affect the results of a Western Blot.

Asked by: TI43
Thank you for your question. This mouse monoclonal antibody, Glut5 (E-2): sc-271055, is not phospho-specific. If an antibody is specific for only the phosphorylated form of a protein, we will describe the phospho-reactivity in the product information, including which phosphorylated residues are specifically targeted.
Answered by: Technical Support
Date published: 2019-08-19

Hello , I have used your Atibody "Glut5 E-2 sc-271055" and it works very nicely, My advisor thinks its too good to be true. Do you have any competing peptide which I could use with this antibody as control, for IHC,and WB.

Asked by: fujeh
Thank you for your question. We do not provide the blocking peptide for this antibody. For additional information, please contact our Technical Service.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-21

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 3
Date published: 2017-02-01
  • y_2025, m_9, d_6, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_4
  • loc_zh_CN, sid_271055, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 107ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Great signal in murine tumor and muscle tissueAntibody was used for immunohistochemistry experiments in paraffin embedded murine breast tumor and murine muscle tissue as well as in western blot experiments, using the same tissues.
Date published: 2018-03-23
Rated 5 out of 5 by from Strong immunofluorescence in rat brain sections1:100 dilution of this antibody was used for immunostaining in 4% PFA fixed brain tissues . it produced very strong fluorescent signal.
Date published: 2017-08-30
Rated 5 out of 5 by from Conclusive + in acute myeloid leukemias (AML)GLUT5 (E-2) antibody (cat# sc-271055, mouse monoclonal antibody) works well in positive control MCF-7 cells as well as acute myeloid leukemia (AML) cell lines including U937, OCI-AML3, HL-60 and K562 (see the attached figure).
Date published: 2017-02-08
Rated 5 out of 5 by from great western blot bandWe used this antibody in a western blot experiment and got a very nice band at the correct molecular weight for HeLa cells
Date published: 2017-01-06
Rated 5 out of 5 by from I have tried the GLUT5 (EI have tried the GLUT5 (E-2) antibody (cat# sc-271055, mouse monoclonal antibody). It works well in positive control MCF-7 cells as well as acute myeloid leukemia (AML) cell lines including U937, OCI-AML3, HL-60 and K562.
Date published: 2015-09-07
Rated 5 out of 5 by from Good WB data in MCF7 and HeLa whole cell lysatesGood WB data in MCF7 and HeLa whole cell lysates. -SCBT QC
Date published: 2015-03-24
Rated 3 out of 5 by from Worked with human transfected lysate by WBWorked with human transfected lysate by WB -SCBT QC
Date published: 2015-02-27
Rated 5 out of 5 by from Membrane and cytoplasmic staining seen in paraffinMembrane and cytoplasmic staining seen in paraffin-embedded human duodenum tissue. -SCBT QC
Date published: 2015-02-17
  • y_2025, m_9, d_6, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_9
  • loc_zh_CN, sid_271055, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 18ms
  • REVIEWS, PRODUCT
Glucose Transporter Glut5 抗体 (E-2) is rated 4.8 out of 5 by 9.
  • y_2025, m_9, d_6, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_9
  • loc_zh_CN, sid_271055, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 106ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。