Date published: 2025-9-8
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GLUD1/2 Antibody (C-10) - Western Blotting - Image 351866
GLUD1/2 抗体 (C-10): sc-515542. K-562 全细胞裂解液 (A), 小鼠大脑 (B) 和小鼠骨骼肌 (C) 组织提取物中 GLUD1/2 表达的 Western 印迹分析.
GLUD1/2 抗体 (C-10): sc-515542. K-562 全细胞裂解液 (A), 小鼠大脑 (B) 和小鼠骨骼肌 (C) 组织提取物中 GLUD1/2 表达的 Western 印迹分析.

GLUD1/2 抗体 (C-10): sc-515542

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说明书
  • GLUD1/2 抗体 C-10 是小鼠单克隆 IgG1 κ，GLUD1/2抗体, 在8篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列1-300位于human物种的GLUD1的N-terminus
  • GLUD1/2 抗体 (C-10) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测 GLUD1 and GLUD2 of human origin, and GLUD1 of mouse and rat origin
  • 抗GLUD1/2抗体（C-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取GLUD1/2 (C-10): sc-515542的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 GLUD1/2 抗体 (C-10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与GLUD1/2 抗体 (C-10) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 GLU 抗体包括 GLUD1 和 GLUD2

GLUD1/2 (C-10) 是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的GLUD1/2蛋白。GLUD1/2 (C-10) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和各种 Alexa Fluor® 形式。GLUD1（谷氨酸脱氢酶1），也称为GDH、GDH1或GLUD，以及GLUD2（谷氨酸脱氢酶2），也称为GDH2或GLUDP1，是线粒体基质酶，属于谷氨酸/亮氨酸/苯丙氨酸/缬氨酸脱氢酶家族。GLUD1以六聚体的形式存在，催化谷氨酸氧化脱氨为α-酮戊二酸和氨，而GLUD2则参与神经传递过程中的谷氨酸循环。GLUD1调节氨基酸诱导的胰岛素分泌，并通过ADP变构激活，通过GTP和ATP抑制。GLUD1的突变会导致高胰岛素血症-高氨血症综合征（HHS），这是一种遗传性疾病，其特征是血液中胰岛素和氨水平过高。GLUD1可能通过增加谷氨酸的代谢来促进学习和记忆。GLUD2主要在睾丸和视网膜中表达，在脑中的表达较低。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

GLUD1/2 抗体 (C-10) 参考文献:

  1. 高胰岛素血症/高氨血症综合征的分子基础和特征：谷氨酸脱氢酶基因第 11 和 12 号外显子的突变占主导地位。HI/HA 参与研究者。  |  Stanley, CA., et al. 2000. Diabetes. 49: 667-73. PMID: 10871207
  2. 谷氨酸脱氢酶活性的非调控性升高诱导谷氨酰胺刺激的胰岛素分泌：GLUD1 基因突变的鉴定和表征，以及过表达突变型谷氨酸脱氢酶的 MIN6 细胞的胰岛素分泌研究。  |  Tanizawa, Y., et al. 2002. Diabetes. 51: 712-7. PMID: 11872671
  3. 高胰岛素血症低血糖诊断和管理的进展。  |  Kapoor, RR., et al. 2009. Nat Clin Pract Endocrinol Metab. 5: 101-12. PMID: 19165222
  4. 影响基础活性和调节的人类 GLUD2 谷氨酸脱氢酶突变。  |  Kanavouras, K., et al. 2009. J Neurochem. 109 Suppl 1: 167-73. PMID: 19393024
  5. 人类 GLUD2 谷氨酸脱氢酶：定位和功能方面。  |  Zaganas, I., et al. 2009. Neurochem Int. 55: 52-63. PMID: 19428807
  6. 人类 GLUD1 和 GLUD2 谷氨酸脱氢酶定位于线粒体和内质网。  |  Mastorodemos, V., et al. 2009. Biochem Cell Biol. 87: 505-16. PMID: 19448744
  7. B 细胞慢性淋巴细胞白血病患者淋巴细胞中的谷氨酸脱氢酶活性。  |  Pajic, T., et al. 2009. Clin Biochem. 42: 1677-84. PMID: 19683518
  8. 高胰岛素血症-高胰岛素血症综合征：GLUD1 基因的新型突变及基因型与表型的相关性。  |  Kapoor, RR., et al. 2009. Eur J Endocrinol. 161: 731-5. PMID: 19690084
  9. GLUD2 谷氨酸脱氢酶的功能增益变异可改变帕金森病的发病。  |  Plaitakis, A., et al. 2010. Eur J Hum Genet. 18: 336-41. PMID: 19826450

订购信息

产品名称产品编号规格价格数量收藏夹

GLUD1/2 抗体 (C-10)

sc-515542
200 µg/ml
RMB2377.00

GLUD1/2 (C-10): m-IgG Fc BP-HRP 套装

sc-531405
200 µg Ab; 10 µg BP
RMB2662.00

GLUD1/2 (C-10): m-IgGκ BP-HRP 套装

sc-525088
200 µg Ab, 40 µg BP
RMB2662.00

GLUD1/2 (C-10): m-IgG1 BP-HRP 套装

sc-544533
200 µg Ab; 20 µg BP
RMB2662.00

GLUD1/2 抗体 (C-10) AC

sc-515542 AC
500 µg/ml, 25% agarose
RMB3129.00

GLUD1/2 抗体 (C-10) HRP

sc-515542 HRP
200 µg/ml
RMB2377.00

GLUD1/2 抗体 (C-10) FITC

sc-515542 FITC
200 µg/ml
RMB2482.00

GLUD1/2 抗体 (C-10) PE

sc-515542 PE
200 µg/ml
RMB2580.00

GLUD1/2 抗体 (C-10) Alexa Fluor® 488

sc-515542 AF488
200 µg/ml
RMB2685.00

GLUD1/2 抗体 (C-10) Alexa Fluor® 546

sc-515542 AF546
200 µg/ml
RMB2685.00

GLUD1/2 抗体 (C-10) Alexa Fluor® 594

sc-515542 AF594
200 µg/ml
RMB2685.00

GLUD1/2 抗体 (C-10) Alexa Fluor® 647

sc-515542 AF647
200 µg/ml
RMB2685.00

GLUD1/2 抗体 (C-10) Alexa Fluor® 680

sc-515542 AF680
200 µg/ml
RMB2685.00

GLUD1/2 抗体 (C-10) Alexa Fluor® 790

sc-515542 AF790
200 µg/ml
RMB2685.00

Is it possible to use this antibody for flow cytometry?

Asked by: mene07
Anti-GLUD1/2 Antibody (C-10) is available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM.
Answered by: Technical Service
Date published: 2023-12-18

For Western Blot, is it recommended to use denatured or non-denatured conditions with GLUD1/2 (C-10): sc-515542 monoclonal antibody?

Asked by: Germaine
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-02-27
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Rated 3 out of 5 by from quite wellwb on mouse brain homogenate: 3 bands, 1 aroud at 50kD seems to be specific
Date published: 2020-01-28
Rated 5 out of 5 by from Excellent antibody!I used this antibody for a western blot of rat liver homogenates. It detected only one band at the correct molecular weight, very clear and clean, without any unspecific bands. I started with a 1:1000 dilution of the antibody, but ended up using a 1:5000 dilution. It works great.
Date published: 2018-07-27
Rated 5 out of 5 by from Good antibodyThis antibody works well in 293T cell lysis with or without transfect GLUD1.
Date published: 2017-09-21
Rated 5 out of 5 by from Sensitive and specificIt works well for mouse liver lysate. ( dilution 1:5000)
Date published: 2017-07-18
Rated 5 out of 5 by from good antibodythis antibody works well, very clear two bands! i recommend it
Date published: 2017-04-17
Rated 5 out of 5 by from works greatwe tested this sample antibody in mouse myobalst cells. it works great
Date published: 2017-03-16
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GLUD1/2 抗体 (C-10) is rated 4.7 out of 5 by 6.
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