Date published: 2025-9-8
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Glucosidase I Antibody (H-6) - Western Blotting - Image 414283
葡萄糖苷酶 I 抗体 (H-6)：sc-365399。HeLa (A)、K-562 (B) 和 HCT-116 (C) 全细胞裂解液中葡萄糖苷酶 I 表达的 Western 印迹分析。所用检测试剂：m-IgG Fc BP-HRP: sc-525409。
葡萄糖苷酶 I 抗体 (H-6)：sc-365399。HeLa (A)、K-562 (B) 和 HCT-116 (C) 全细胞裂解液中葡萄糖苷酶 I 表达的 Western 印迹分析。所用检测试剂：m-IgG Fc BP-HRP: sc-525409。

Glucosidase I 抗体 (H-6): sc-365399

5.0(2)
寫評論提問

说明书
  • Glucosidase I抗体(H-6)是小鼠单克隆IgG1 (kappa轻链)，规格为200 µg/ml
  • 针对human起源的Glucosidase I内部区域内的氨基酸395-694进行
  • 推荐用于 mouse, rat 和 human 来源的Glucosidase I WB, IP, IF, IHC(P) 和 ELISA检测
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是Glucosidase I Antibody (H-6) for WB and IHC(P) applications. 的首选辅助检测试剂，这些试剂现与Glucosidase I Antibody (H-6) 打包提供（请参阅下面的订购信息）。

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    描述
    基因信息
    蛋白序列
    说明书与实验方案
    研究信息

    関連項目

    额外Glucosidase抗体，包括Glucosidase II β

    蛋白质中 Asn-X-Ser/Thr 基序的天冬酰胺残基的糖基化通常发生在内质网（ER）腔中。Glucosidase I 催化 N-连接寡糖加工途径的第一步。它特异性地从 Glc3-Man9-GlcNAc2 寡糖前体中移除远端的 alpha 1,2-连接的葡萄糖残基。Glucosidase I 包含一个短的胞质尾，一个单次跨膜结构域和一个位于 ER 腔侧的大 C 端催化结构域。编码 Glucosidase I 的基因突变会导致先天性糖基化紊乱（CDG-IIb），其特征是全身性低张力、畸形特征、肝肿大、低通气、喂养问题、癫痫发作和死亡。已在 Glucosidase I 基因中鉴定出两个点突变，导致氨基酸替换，即 Arg486Thr 和 Phe652Leu，这些替换会影响多肽折叠和活性位点形成。

    仅限研究使用。不适用于诊断和治疗用途。

    订购信息

    产品名称产品编号规格价格数量收藏夹

    Glucosidase I 抗体 (H-6)

    sc-365399
    200 µg/ml
    		RMB2377.00

    Glucosidase I (H-6): m-IgG Fc BP-HRP 套装

    sc-540338
    200 µg Ab; 10 µg BP
    		RMB2662.00

    Glucosidase I (H-6): m-IgG1 BP-HRP 套装

    sc-542051
    200 µg Ab; 20 µg BP
    		RMB2662.00

    Hello, i work with S. pombe and T. cruzi as model organisms. I need a GI antibody for both western blot and immunofluorescence. I ran structural alignments and despite not having aminoacidic identity (25%), structural is 80%. Would this Ab work for me?

    Asked by: Tommy
    Thank you for your question. sc-365399 Glucosidase I (H-6) is recommended for western blot, IP and ELISA in mouse,rat and human samples .
    Answered by: BlakeJ
    Date published: 2025-01-16

    Hello, i bought this antibody a couple of months ago and i was wondering what amount of total protein do you use from the cells lysate to detect presence of GI. I tried with 30ug and couldn't get signal.

    Asked by: Tommy
    Thank you for your question. We use the following amounts in western blots, depending on sample type: 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane.
    Answered by: Tech Support Europe
    Date published: 2024-12-11

    Can Glucosidase I (H-6): sc-365399 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

    Asked by: Trav11
    Thank you for your question. Yes, Glucosidase I (H-6): sc-365399 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
    Answered by: Technical Support
    Date published: 2017-03-01
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    Rated 5 out of 5 by from Produced superb immunoperoxidase cytoplasmicProduced superb immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human duodenum tissue. -SCBT QC
    Date published: 2015-04-15
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    Glucosidase I 抗体 (H-6) is rated 5.0 out of 5 by 2.
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