Date published: 2025-9-6
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GLI-2 Antibody (C-10) - Western Blotting - Image 136360
GLI-2 抗体 (C-10): sc-271786. F9 (A) 和 K-562 (B) 全细胞裂解液中 GLI-2 表达的 Western 印迹分析.
GLI-2 抗体 (C-10): sc-271786. F9 (A) 和 K-562 (B) 全细胞裂解液中 GLI-2 表达的 Western 印迹分析.

GLI-2 抗体 (C-10): sc-271786

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说明书
  • GLI-2 抗体 C-10 是小鼠单克隆 IgG1 κ，GLI-2抗体, 在56篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列841-1140位于human物种的GLI-2的C-terminus附近
  • GLI-2 抗体 (C-10) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 GLI-2
  • 抗GLI-2抗体（C-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-271786 X, 200 µg/0.1 ml)
  • 关于如何获取GLI-2 (C-10): sc-271786的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 1 BP-HRP">m-IgG1 BP-HRP是GLI-2 Antibody (C-10) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。该试剂现与GLI-2 Antibody (C-10) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

附加GLI抗体，包括GLI-1, GLI-2和GLI-3

GLI-2抗体（C-10）是一种IgG1 κ小鼠单克隆GLI-2抗体（也被指定为GLI2抗体），能够通过WB、IP、IF、IHC(P)和ELISA检测到小鼠、大鼠和人类来源的GLI-2蛋白。GLI-2抗体（C-10）既有非偶联型抗GLI-2抗体形式，也有多种偶联型抗GLI-2抗体形式，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。锌指蛋白包含DNA结合域，并具有多种多样的功能，其中大多数包括某种形式的转录激活或抑制。大多数锌指蛋白含有一个Krüppel型DNA结合域和一个KRAB域，据认为能够与KAP1相互作用，从而募集组蛋白修饰蛋白。GLI-2（GLI家族锌指2），也被称为HPE9或THP（辅助蛋白），是一种含有1586个氨基酸的核蛋白，充当转录激活剂，属于GLI C2H2型锌指蛋白家族。GLI-2定位于细胞核，据认为在胚胎发育中发挥作用。编码GLI-2的基因定位于人类2号染色体长臂1区4带2亚带（2q14.2），该基因缺陷是导致第9型前脑无裂畸形（HPE9）的原因。GLI-2存在五种选择性剪接的同种型。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

GLI-2 抗体 (C-10) 参考文献:

  1. 人类 GLI2 基因的功能缺失突变与垂体异常和全脑样特征缔合作用。  |  Roessler, E., et al. 2003. Proc Natl Acad Sci U S A. 100: 13424-9. PMID: 14581620
  2. 一个先前未被发现的氨基末端结构域调控着野生型和与疾病相关的人类 GLI2 的转录活性。  |  Roessler, E., et al. 2005. Hum Mol Genet. 14: 2181-8. PMID: 15994174
  3. 人类锌指蛋白家族，可结合甲基化 DNA 并抑制转录。  |  Filion, GJ., et al. 2006. Mol Cell Biol. 26: 169-81. PMID: 16354688
  4. [KRAB 锌指蛋白研究进展]。  |  Tian, CY., et al. 2006. Yi Chuan. 28: 1451-6. PMID: 17098717
  5. 鉴定新型丝氨酸/苏氨酸激酶 ULK3 作为刺猬通路的正调控因子  |  Maloverjan, A., et al. 2010. Exp Cell Res. 316: 627-37. PMID: 19878745
  6. 骨肉瘤中Hedgehog-GLI-2通路的靶向治疗。  |  Yang, W., et al. 2013. J Orthop Res. 31: 502-9. PMID: 22968906
  7. 乳腺癌中Hedgehog相关蛋白的表达：gli-2与总体生存率低相关。  |  Im, S., et al. 2013. Korean J Pathol. 47: 116-23. PMID: 23667370
  8. 缺氧通过MEKK1/MEK1/ERK1/GLI-1/GLI-2和AP-1通路诱导人肺成纤维细胞中结缔组织生长因子的表达。  |  Cheng, Y., et al. 2016. PLoS One. 11: e0160593. PMID: 27486656
  9. Hedgehog通路相关蛋白Gli-1和Gli-2以及上皮-间质转化相关蛋白Twist和E-cadherin在肝细胞癌中的意义。  |  Chun, HW. and Hong, R. 2016. Oncol Lett. 12: 1753-1762. PMID: 27602109
  10. 人类基因 GLI-Kruppel 家族。  |  Ruppert, JM., et al. 1988. Mol Cell Biol. 8: 3104-13. PMID: 2850480
  11. 增强人类 T 细胞白血病病毒 1 型 Tax 依赖性增强子的新调控元件以及编码其结合蛋白的 cDNA 的克隆。  |  Tanimura, A., et al. 1993. J Virol. 67: 5375-82. PMID: 8350401
  12. 克隆人类 Gli2 肿瘤基因的新型异构体及其增强人类 T 细胞白血病病毒 1 型基因组的税收依赖性转录的活性。  |  Tanimura, A., et al. 1998. J Virol. 72: 3958-64. PMID: 9557682

订购信息

产品名称产品编号规格价格数量收藏夹

GLI-2 抗体 (C-10)

sc-271786
200 µg/ml
RMB2377.00

GLI-2 (C-10): m-IgG1 BP-HRP 套装

sc-531692
200 µg Ab; 20 µg BP
RMB2662.00

GLI-2 抗体 (C-10) X

sc-271786 X
200 µg/0.1 ml
RMB2377.00

GLI-2 抗体 (C-10) AC

sc-271786 AC
500 µg/ml, 25% agarose
RMB3129.00

GLI-2 抗体 (C-10) HRP

sc-271786 HRP
200 µg/ml
RMB2377.00

GLI-2 抗体 (C-10) FITC

sc-271786 FITC
200 µg/ml
RMB2482.00

GLI-2 抗体 (C-10) PE

sc-271786 PE
200 µg/ml
RMB2580.00

GLI-2 抗体 (C-10) Alexa Fluor® 488

sc-271786 AF488
200 µg/ml
RMB2685.00

GLI-2 抗体 (C-10) Alexa Fluor® 546

sc-271786 AF546
200 µg/ml
RMB2685.00

GLI-2 抗体 (C-10) Alexa Fluor® 594

sc-271786 AF594
200 µg/ml
RMB2685.00

GLI-2 抗体 (C-10) Alexa Fluor® 647

sc-271786 AF647
200 µg/ml
RMB2685.00

GLI-2 抗体 (C-10) Alexa Fluor® 680

sc-271786 AF680
200 µg/ml
RMB2685.00

GLI-2 抗体 (C-10) Alexa Fluor® 790

sc-271786 AF790
200 µg/ml
RMB2685.00

Hi, I am looking for a Gli2 antibody. Can I get some sc-271786 and sc-20291 to try on our samples?

Asked by: bchen
Thank you for your question. Please contact Technical Service for more information on how to receive a 10 μg sample of our well characterized monoclonal antibody.
Answered by: Technical Support
Date published: 2018-12-05

When should the TransCruz reagent sc-271786 X be used instead of the standard form sc-271786?

Asked by: jenni
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. GLI-2 Antibody (C-10): sc-271786 X form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support 4
Date published: 2017-01-09
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Rated 2 out of 5 by from no good signal in IFI didn't detect good staining signal in 4%PFA fixed embyronic mouse brain(after antigen retrieval)
Date published: 2018-03-07
Rated 1 out of 5 by from Do not work in IFI have not succeed to make it work in IF, with MetOH or PFA fixation, on RPE-1 cells activated or not with SAG.
Date published: 2018-03-05
Rated 4 out of 5 by from GOOD FOR IPI got a nice signals after immunoprecipitation from murine embryonic fibroblast.
Date published: 2017-11-01
Rated 5 out of 5 by from The results reflect a formal situation.抗体的效价和滴度都满足要求，能够客观呈现蛋白水平，我很高兴能获得这样的生化试剂帮助我完成实验，会继续购买！
Date published: 2017-09-21
Rated 3 out of 5 by from specificity in WB?nice band but detected around 100 kDa only in fibroblasts whole lysate and nuclei. short isoform only? Do not correspond to your picture
Date published: 2017-02-27
Rated 4 out of 5 by from can be used for IFWe used this antibody(GLI-2 (C-10)：sc-271786) to detect Gli2 in mouse liver by IF analysis and got a good result.
Date published: 2017-02-23
Rated 5 out of 5 by from used the known conditionsWe used the known conditions that most zinc finger proteins contain the Krüppel type DNA binding domain and the KRAB domain, by virtue of their interaction with KAP1, thereby recruiting histones for enzymatic enzymatic oxygenation reactions.
Date published: 2017-02-12
Rated 5 out of 5 by from Best WB and IF antibodyGreat results; very clear highly specific signal, works great
Date published: 2017-01-10
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GLI-2 抗体 (C-10) is rated 4.2 out of 5 by 14.
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