Date published: 2025-9-7
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GKLF/EKLF/LKLF/KLF4/1/2 Antibody (F-8) - Western Blotting - Image 403553
GKLF/EKLF/LKLF/KLF4/1/2 抗体 (F-8): sc-166238. 非转染: sc-117752 (A) 和人 GKLF 转染: sc-114641 (B) 293T 全细胞裂解液中 GKLF/EKLF/LKLF 表达的近红外 western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgGκ BP-CFL 790: sc-516181.
GKLF/EKLF/LKLF/KLF4/1/2 抗体 (F-8): sc-166238. 非转染: sc-117752 (A) 和人 GKLF 转染: sc-114641 (B) 293T 全细胞裂解液中 GKLF/EKLF/LKLF 表达的近红外 western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgGκ BP-CFL 790: sc-516181.

GKLF/EKLF/LKLF/KLF4/1/2 抗体 (F-8): sc-166238

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说明书
  • GKLF/EKLF/LKLF/KLF4/1/2抗体(F-8)是小鼠单克隆IgG1 κ, 在18篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于mouse物种的GKLF的C-terminus的氨基酸449-481之间
  • GKLF/EKLF/LKLF/KLF4/1/2 抗体 (F-8) 推荐用于WB, IP, IF 和 ELISA ，检测 mouse, rat, human 和 来源的multiple Kruppel-like factors including GKLF, EKLF and LKLF; 也和以下物种反应，包括： 和 canine, bovine, porcine and avian
  • 抗GKLF/EKLF/LKLF/KLF4/1/2抗体（F-8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-166238 X, 200 µg/0.1 ml)
  • 关于如何获取GKLF/EKLF/LKLF/KLF4/1/2 (F-8): sc-166238的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是GKLF/EKLF/LKLF/KLF4/1/2 Antibody (F-8) for WB applications. 的首选辅助检测试剂，这些试剂现与GKLF/EKLF/LKLF/KLF4/1/2 Antibody (F-8) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

GKLF/EKLF/LKLF/KLF4/1/2抗体（F-8）是一种IgG1 κ小鼠单克隆GKLF/EKLF/LKLF抗体（也称为KLF4 KLF1 KLF2抗体），它可以通过WB、IP、IF和ELISA检测到包括小鼠、大鼠和人类的GKLF、EKLF和LKLF在内的多种Kruppel样因子。GKLF/EKLF/LKLF/KLF4/1/2抗体（F-8）既可作为非共轭抗GKLF/EKLF/LKLF抗体形式，也可作为多种共轭形式的抗GKLF/EKLF/LKLF抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。Kruppel型锌指转录因子是一个保守的DNA结合蛋白家族，在发育调控中起重要作用。Kruppel锌指转录因子最初在果蝇中被鉴定为分段基因。在哺乳动物中已鉴定的Kruppel样因子包括EKLF、LKLF和GKLF。EKLF主要在红细胞组织中表达，而LKLF的表达仅限于肺。GKLF主要存在于肠道中，并已被证明在生长停滞期间表达。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

GKLF/EKLF/LKLF/KLF4/1/2 抗体 (F-8) 参考文献:

  1. 胸腺上皮中的GKLF是胸腺细胞-基质相互作用的发育调节因子。  |  Panigada, M., et al. 1999. Mech Dev. 81: 103-13. PMID: 10330488
  2. 乳腺癌进展过程中GKLF信使RNA和蛋白表达的增加。  |  Foster, KW., et al. 2000. Cancer Res. 60: 6488-95. PMID: 11103818
  3. 肠道富集的Kruppel样因子（GKLF/KLF4）和Sp1协同激活大鼠层粘连蛋白γ1链启动子。  |  Higaki, Y., et al. 2002. Nucleic Acids Res. 30: 2270-9. PMID: 12034813
  4. Krüppel样因子4（KLF4/GKLF）是骨形态发生蛋白和转化生长因子β1在调节血管平滑肌细胞表型中的靶标。  |  King, KE., et al. 2003. J Biol Chem. 278: 11661-9. PMID: 12538588
  5. 编码锌指蛋白的人类基因的特征和图谱。  |  Bray, P., et al. 1991. Proc Natl Acad Sci U S A. 88: 9563-7. PMID: 1946370
  6. 编码锌指结构的小鼠多基因家族的特征。  |  Chavrier, P., et al. 1988. Mol Cell Biol. 8: 1319-26. PMID: 2452975
  7. 人类基因 GLI-Kruppel 家族。  |  Ruppert, JM., et al. 1988. Mol Cell Biol. 8: 3104-13. PMID: 2850480
  8. 包含果蝇分节基因 Krüppel 编码的指状蛋白结构元素的核蛋白保守家族。  |  Schuh, R., et al. 1986. Cell. 47: 1025-32. PMID: 3096579
  9. 在杆状病毒表达系统中产生的果蝇 Krüppel 基因产物是一种能与 DNA 结合的核磷蛋白。  |  Ollo, R. and Maniatis, T. 1987. Proc Natl Acad Sci U S A. 84: 5700-4. PMID: 3112773
  10. 分离出编码与红细胞 Krüppel 样因子同源的功能性锌指蛋白的基因：确定了一个新的多基因家族。  |  Anderson, KP., et al. 1995. Mol Cell Biol. 15: 5957-65. PMID: 7565748
  11. 鉴定和表征一种在生长停滞期间表达的肠道富集克鲁珀尔样因子的编码基因。  |  Shields, JM., et al. 1996. J Biol Chem. 271: 20009-17. PMID: 8702718
  12. 人类红细胞克鲁佩尔样因子（EKLF）的分离, 基因组结构和表达。  |  Bieker, JJ. 1996. DNA Cell Biol. 15: 347-52. PMID: 8924208

订购信息

产品名称产品编号规格价格数量收藏夹

GKLF/EKLF/LKLF/KLF4/1/2 抗体 (F-8)

sc-166238
200 µg/ml
RMB2377.00

GKLF/EKLF/LKLF/KLF4/1/2 (F-8): m-IgG Fc BP-HRP 套装

sc-527146
200 µg Ab; 10 µg BP
RMB2662.00

GKLF/EKLF/LKLF/KLF4/1/2 (F-8): m-IgG1 BP-HRP 套装

sc-532519
200 µg Ab; 20 µg BP
RMB2662.00

GKLF/EKLF/LKLF/KLF4/1/2 抗体 (F-8) X

sc-166238 X
200 µg/0.1 ml
RMB2377.00

GKLF/EKLF/LKLF/KLF4/1/2 抗体 (F-8) AC

sc-166238 AC
500 µg/ml, 25% agarose
RMB3129.00

GKLF/EKLF/LKLF/KLF4/1/2 抗体 (F-8) HRP

sc-166238 HRP
200 µg/ml
RMB2377.00

GKLF/EKLF/LKLF/KLF4/1/2 抗体 (F-8) FITC

sc-166238 FITC
200 µg/ml
RMB2482.00

GKLF/EKLF/LKLF/KLF4/1/2 抗体 (F-8) PE

sc-166238 PE
200 µg/ml
RMB2580.00

GKLF/EKLF/LKLF/KLF4/1/2 抗体 (F-8) Alexa Fluor® 488

sc-166238 AF488
200 µg/ml
RMB2685.00

GKLF/EKLF/LKLF/KLF4/1/2 抗体 (F-8) Alexa Fluor® 546

sc-166238 AF546
200 µg/ml
RMB2685.00

GKLF/EKLF/LKLF/KLF4/1/2 抗体 (F-8) Alexa Fluor® 594

sc-166238 AF594
200 µg/ml
RMB2685.00

GKLF/EKLF/LKLF/KLF4/1/2 抗体 (F-8) Alexa Fluor® 647

sc-166238 AF647
200 µg/ml
RMB2685.00

GKLF/EKLF/LKLF/KLF4/1/2 抗体 (F-8) Alexa Fluor® 680

sc-166238 AF680
200 µg/ml
RMB2685.00

GKLF/EKLF/LKLF/KLF4/1/2 抗体 (F-8) Alexa Fluor® 790

sc-166238 AF790
200 µg/ml
RMB2685.00

GKLF/EKLF/LKLF/KLF4/1/2 (F-8) 中和勝肽

sc-166238 P
100 µg/0.5 ml
RMB511.00

I'm looking for KLF2 antibody in your website since many research groups cited the KLF2 antibody from your company. But I'm not able to find one. Could you recommend one for me? Thanks

Asked by: Yuxia
Thank you for your question. We currently do not offer a KLF2-specific antibody in our portfolio.
Answered by: Tech Support Europe
Date published: 2020-08-05

Could you please refer me to the technical service department? I ordered an antibody today I want to get three free samples with that. Thanks!

Asked by: Sajedeh
Thank you for your question. Please reach out to technical service directly. You can find our contact information here: https://www.scbt.com/resources/technical-service
Answered by: Tech Service
Date published: 2019-10-23

Hi. I want to order an antibody. I was wondering how can I get free samples for three more antibodies?

Asked by: Sajedeh
Thank you for your question. Please contact our Technical Service Department for more information on how to receive free 10 ug samples with your purchase. We are happy to make you a quote to ensure your samples ship with your order!
Answered by: Tech Service
Date published: 2021-06-28

What application is the blocking peptide sc-166238 P appropriate for?

Asked by: AbPolly
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 2 out of 5 by from Segnale basso e molti aspecificiHo provato l'anticorpo in western blot e fa molte bande con un segnale molto basso
Date published: 2024-02-02
Rated 5 out of 5 by from Good for WBThe antibody is effective for SDS immunoblotting. To dilute the primary antibody, we used a ratio of 1:500 in a mixture of 5% nonfat dry milk, 1X TBS, and 0.1% Tween® 20. The antibody was left overnight at 4°C.
Date published: 2023-11-07
Rated 5 out of 5 by from Mouse Klf4 detection by western blottingMouse Klf4 connected with Venus and P2A was overexpressed in Lenti-X 293T cells, a derivative of HEK293T. Mouse Klf4 was clearly detected by Venus-P2A-Klf4 overexpression. Membrane was blocked with 5% Skim-milk/TBST and the antibody was diluted with 5% BSA/TBST.
Date published: 2019-02-22
Rated 5 out of 5 by from Great antibodyThis is antibody works great in our cell lines. Very clear band.
Date published: 2017-12-07
Rated 5 out of 5 by from Good Impression for UsVery stable and very good quality for IHC staining. Used in the cell analysis of this antibody, it has strong staining.
Date published: 2017-10-28
Rated 1 out of 5 by from bad featuresI didn't detect any bands in WB analysis of 293T lysate. However, i haven't tried other lysate yet.
Date published: 2017-09-18
Rated 4 out of 5 by from sample trial feedbackI got a bunch of sample from santa cruz couple weeks ago and I tested this antibody in vascular smooth muscle cells for western blot. The results were good.
Date published: 2017-08-11
Rated 4 out of 5 by from goodI chose the dilution 1:500 for WB and I‘ve achieved such better research results.I'm here to show my acknowledgement and It deserves to be appreciated
Date published: 2017-08-07
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GKLF/EKLF/LKLF/KLF4/1/2 抗体 (F-8) is rated 4.4 out of 5 by 15.
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