Date published: 2025-9-7
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GFRα-1 Antibody (E-11) - Western Blotting - Image 356929
GFRα-1 抗体 (E-11): sc-271546. H4 (A), Caki-1 (B), C2C12 (C), NIH/3T3 (D), C6 (E) 和 NRK (F) 全细胞裂解液中 GFRα-1 表达的 Western 印迹分析.
GFRα-1 抗体 (E-11): sc-271546. H4 (A), Caki-1 (B), C2C12 (C), NIH/3T3 (D), C6 (E) 和 NRK (F) 全细胞裂解液中 GFRα-1 表达的 Western 印迹分析.

GFRα-1 抗体 (E-11): sc-271546

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寫評論提問

说明书
  • GFR alpha-1 抗体 E-11 是小鼠单克隆 IgG1 κ，GFRα-1抗体, 在33篇文献中引用，规格为200 µg/ml
  • 免疫human物种的GFRα-1的氨基酸368-437
  • GFR alpha-1 抗体 (E-11) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 GFRα-1
  • 抗GFR alpha-1抗体（E-11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取GFRα-1 (E-11): sc-271546的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是GFRα-1 Antibody (E-11) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与GFRα-1 Antibody (E-11) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
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说明书与实验方案
研究信息

関連項目

附加GFR α抗体，包括GFR α-1和GFR α-3

GFRα-1抗体（E-11）是一种IgG1κ小鼠单克隆GFRα-1抗体（也被称为GDNF家族受体α1抗体、RETL1抗体、TRNR1抗体、转化生长因子β相关神经营养因子受体1抗体、GDNF家族受体α-1抗体、GDNFR-α-1抗体、RET配体1抗体、GFR-ALPHA-1抗体、GDNFRA抗体、GDNFR抗体、RET1L抗体或神经胶质细胞系来源神经营养因子受体α抗体），可以通过WB、IP、IF、IHC（P）和ELISA等方法检测小鼠、大鼠和人类来源的GFRα-1蛋白。GFRα-1抗体（E-11）有非偶联的抗GFRα-1抗体形式，也有多种偶联形式的抗GFRα-1抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。神经胶质细胞系来源神经营养因子（GDNF）和相关的神经营养因子neurturin（NTN）是中枢和外周神经元的有力存活因子。GDNF是一种糖基化、二硫键连接的同源二聚体，与转化生长因子β超家族的生长因子关系较远。已经确定了这三种因子的受体：GFRα-1（也被称为GDNFR-α、RETL1或TrnR-1）、GFRα-2（也被称为GDNFR-β、RETL2、NTNR-α或TrnR-2）和GFRα-3。这些受体不包含跨膜域，而是通过糖基-磷酰肌醇链接附着在细胞膜上。已经证明，GFRα-1和GFRα-2都可以介导GDNF依赖性和NTN依赖性的酪氨酸激酶Ret的磷酸化和激活。GFRα-3仅在发育过程中表达。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

GFRα-1 抗体 (E-11) 参考文献:

  1. 黑质和腹侧被盖区多巴胺能和非多巴胺能神经元中的GFRalpha-1 mRNA。  |  Sarabi, A., et al. 2001. J Comp Neurol. 441: 106-17. PMID: 11745638
  2. 在没有Ret的情况下，通过GFRalpha-1（而非GFRalpha-2）进行配体特异性信号传导的证据。  |  Pezeshki, G., et al. 2001. J Neurosci Res. 66: 390-5. PMID: 11746356
  3. XIB4035，一种新型非肽类小分子GFRalpha-1激动剂。  |  Tokugawa, K., et al. 2003. Neurochem Int. 42: 81-6. PMID: 12441171
  4. 体内海马AAV1-Rheb(S16H)给药后GDNF和GFRα-1的诱导。  |  Yun, D., et al. 2020. Exp Neurobiol. 29: 164-175. PMID: 32408406
  5. GFRα-1是牛生殖细胞/未分化精原细胞的可靠标记：小型综述。  |  Zhang, XM. 2021. Anat Histol Embryol. 50: 13-14. PMID: 32761645
  6. 泪腺腺样囊性癌患者中异常表达的GFRα-1/RET与高复发风险有关：51例LACC的回顾性研究。  |  Liu, L., et al. 2021. Cancer Biol Med. 18: 199-205. PMID: 33628594
  7. GDNF：中脑多巴胺能神经元的神经胶质细胞系衍生神经营养因子。  |  Lin, LF., et al. 1993. Science. 260: 1130-2. PMID: 8493557
  8. GDNF 多成分受体的特征。  |  Treanor, JJ., et al. 1996. Nature. 382: 80-3. PMID: 8657309
  9. GDNF诱导的ret蛋白酪氨酸激酶激活是由GDNF的新型受体GDNFR-α介导的。  |  Jing, S., et al. 1996. Cell. 85: 1113-24. PMID: 8674117
  10. 神经营养素（Neurturin）是神经胶质细胞系衍生神经营养因子的一种亲缘因子。  |  Kotzbauer, PT., et al. 1996. Nature. 384: 467-70. PMID: 8945474
  11. TrnR2是一种新型受体，可通过Ret介导新神经营养素和GDNF信号。  |  Baloh, RH., et al. 1997. Neuron. 18: 793-802. PMID: 9182803
  12. 神经胶质细胞系衍生物神经营养因子家族受体 GFRalpha3 的表达和调节。  |  Naveilhan, P., et al. 1998. Proc Natl Acad Sci U S A. 95: 1295-300. PMID: 9448325

订购信息

产品名称产品编号规格价格数量收藏夹

GFRα-1 抗体 (E-11)

sc-271546
200 µg/ml
RMB2377.00

GFRα-1 (E-11): m-IgG Fc BP-HRP 套装

sc-529207
200 µg Ab; 10 µg BP
RMB2662.00

GFRα-1 (E-11): m-IgGκ BP-HRP 套装

sc-521975
200 µg Ab, 40 µg BP
RMB2662.00

GFRα-1 (E-11): m-IgG1 BP-HRP 套装

sc-543385
200 µg Ab; 20 µg BP
RMB2662.00

GFRα-1 抗体 (E-11) AC

sc-271546 AC
500 µg/ml, 25% agarose
RMB3129.00

GFRα-1 抗体 (E-11) HRP

sc-271546 HRP
200 µg/ml
RMB2377.00

GFRα-1 抗体 (E-11) FITC

sc-271546 FITC
200 µg/ml
RMB2482.00

GFRα-1 抗体 (E-11) PE

sc-271546 PE
200 µg/ml
RMB2580.00

GFRα-1 抗体 (E-11) Alexa Fluor® 488

sc-271546 AF488
200 µg/ml
RMB2685.00

GFRα-1 抗体 (E-11) Alexa Fluor® 546

sc-271546 AF546
200 µg/ml
RMB2685.00

GFRα-1 抗体 (E-11) Alexa Fluor® 594

sc-271546 AF594
200 µg/ml
RMB2685.00

GFRα-1 抗体 (E-11) Alexa Fluor® 647

sc-271546 AF647
200 µg/ml
RMB2685.00

GFRα-1 抗体 (E-11) Alexa Fluor® 680

sc-271546 AF680
200 µg/ml
RMB2685.00

GFRα-1 抗体 (E-11) Alexa Fluor® 790

sc-271546 AF790
200 µg/ml
RMB2685.00

The size of the GFRA1 protein shown in your company's antibody display result diagram is much larger than the predicted protein size by about 50kDa. How to explain it?

Asked by: ninijo
Thank you for your question. A large molecular weight may indicate glycosylated GFRa-1.
Answered by: Ling Chen
Date published: 2024-11-21

I would like to know if there are any antibodies against GFR alpha available with avian reactivity. Thanks in advance.

Asked by: GNSL
Thank you for your question. We do not currently recommend any of our GFRalpha-1 antibodies for use in avian samples. However, we haven't tested them for reactivity in that species. Please contact our Technical Service Department and we could help you perform sequence analysis to predict potential cross-reactivity with our antibodies and your avian species of interest.
Answered by: Technical Support
Date published: 2019-03-07

Do this antibody(E-11) stain phospho-form of GFRa-1 receptor?

Asked by: clear46
Thank you for your question. This antibody does not distinguish between the phosphylated and non-phophorylated form on the protein, and will stain whichever form is present in the sample.
Answered by: Tech Service
Date published: 2019-01-17

We would like to know what it means the E-11 in the antibody name. Thank you

Asked by: trubilar
Thank you for your question. The "(E-11)" is the internal clone designation for this mouse monoclonal, GFRα-1 Antibody (E-11): sc-271546. It does not have any specific meaning, other than to be a unique identifier for this particular clone.
Answered by: Technical Support
Date published: 2017-03-28

I am using GFRα-1 (E-11): sc-271546 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: DefinitelyNotMatt
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Very potent!I bought this two weeks ago and it has stained my cells very well at a 1:200 dilution! Would highly recommend for cell culture work!
Date published: 2023-04-11
Rated 5 out of 5 by from Detects specific band in T47D breast cancer cells1:1000 dilution was able to detect a clean band at around 78 kDa (presumably the glycosylated form) from 50 ug T47D breast cancer cell lysate.
Date published: 2017-04-21
Rated 1 out of 5 by from Unsuccessful detection of GFRa1 by WB or IFThe antibody was unable to detect GFRa1 in western blots of whole cell lysates of HeLa cells transfected with GFRa1, or SH-SY5Y cells (with endogenous GFRa1 expression). The recommended 1:1000 dilution was used. Background bands of incorrect size appeared in all lanes, including negative controls. Immunofluorescence of SH-SY5Y cells, or HeLa cells transfected with GFRa1 resulted in off-target effects, high background, and no detectable GFRa1. The recommended 1:50 dilution was used.
Date published: 2017-03-30
Rated 5 out of 5 by from Strong positive signalStrong positive signal produced with GFR alpha-1 Antibody (E-11) by WB, IHCP and IF
Date published: 2017-01-31
Rated 5 out of 5 by from WB correct mw sized bandDistinct band observed by WB on 10 ug Caki2 whole cell lysates at 1:1000 dilution.
Date published: 2016-12-12
Rated 5 out of 5 by from Robust signal observed in WB in HeLa wholeRobust signal observed in WB in HeLa whole cell lysate. -SCBT QC
Date published: 2015-03-27
Rated 5 out of 5 by from Produced beautiful immunofluorescence cytoplasmicProduced beautiful immunofluorescence cytoplasmic and membrane staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-02-05
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GFRα-1 抗体 (E-11) is rated 4.4 out of 5 by 7.
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