HPLC Analysis and UV Spectrum Conditions: The HPLC chromatogram (205 and 254 nm) was generated on C18 reverse phase (Alltima, Rocket format, Grace Discovery) under gradient conditions (0 to 100% ACN, 0.01% TFA, 10 min). Germicidin B eluted as a single peak at 4.37 min displaying UVmax at 203 and 289 nm with no related or unrelated co-metabolites observed. Purity for batch >98% when analysed within the linear concentration/peak height response range.Exp. Date: 4/3/2023