Date published: 2025-9-8

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Gephyrin 抗体 (B-4): sc-55469

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说明书
  • Gephyrin抗体(B-4)是小鼠单克隆IgG2b (kappa轻链),规格为200 µg/ml
  • 免疫rat物种的Gephyrin的氨基酸437-736
  • 推荐用于 mouse, rat 和 human 来源的Gephyrin WB, IP, IF 和 ELISA检测; 也和以下物种反应,包括: 和 equine and canine
  • m-IgG Fc BP-HRP2b BP-HRP">m-IgG2b BP-HRPm-IgGκ BP-HRP是Gephyrin Antibody (B-4) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Gephyrin Antibody (B-4) 打包提供(请参阅下面的订购信息)。

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    突触后膜的亚膜区含有许多对受体靶向至关重要的蛋白质。Gephyrin是一种微管相关蛋白,在脑中高度表达,并定位于神经元突触后膜。Gephyrin对于抑制性甘氨酸受体的突触后定位至关重要,被认为是将受体锚定到突触下微管。该蛋白在大多数哺乳动物组织中表达,主要在脑中表达。在大鼠和人脑组织中,已经鉴定出至少五个分子量不同的Gephyrin剪接变异体。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    Gephyrin 抗体 (B-4) 参考文献:

    1. 受体、gephyrin和gephyrin相关蛋白:抑制性突触后膜特化组装的新见解。  |  Kneussel, M. and Betz, H. 2000. J Physiol. 525 Pt 1: 1-9. PMID: 10811719
    2. GABA(A)受体、gephyrin和稳态突触可塑性。  |  Tyagarajan, SK. and Fritschy, JM. 2010. J Physiol. 588: 101-6. PMID: 19752109
    3. Gephyrin磷酸化在GABA能突触的功能组织和可塑性中的作用。  |  Zacchi, P., et al. 2014. Front Cell Neurosci. 8: 103. PMID: 24782709
    4. Gephyrin与甘氨酸能抑制性突触的突触强度和动态调节。  |  Alvarez, FJ. 2017. Brain Res Bull. 129: 50-65. PMID: 27612963
    5. 甘氨酸和GABAA受体的结构-功能关系及其与支架蛋白Gephyrin的相互作用。  |  Kasaragod, VB. and Schindelin, H. 2018. Front Mol Neurosci. 11: 317. PMID: 30258351
    6. Gephyrin与微管的结合受其在Ser270处的磷酸化调节。  |  Zhou, L., et al. 2021. Histochem Cell Biol. 156: 5-18. PMID: 33796945
    7. 新皮质锥体神经元上缺乏Gephyrin的PV突触。  |  Kuljis, DA., et al. 2021. Int J Mol Sci. 22: PMID: 34576197
    8. 解读gephyrin介导的collybistin激活的构象动力学。  |  Imam, N., et al. 2022. Biophys Rep (N Y). 2: 100079. PMID: 36425671
    9. Gephyrin磷酸化促进小鼠海马中性二态性发育和微球蛋白中间神经元的功能。  |  Campbell, BFN., et al. 2024. Mol Psychiatry. 29: 2510-2526. PMID: 38503929
    10. Gephyrin促进GABA能突触后成分的自主组装和突触定位,无需突触前GABA释放。  |  Carricaburu, E., et al. 2024. Proc Natl Acad Sci U S A. 121: e2315100121. PMID: 38889143

    订购信息

    产品名称产品编号规格价格数量收藏夹

    Gephyrin 抗体 (B-4)

    sc-55469
    200 µg/ml
    $316.00

    Gephyrin (B-4): m-IgG Fc BP-HRP 套装

    sc-537040
    200 µg Ab; 10 µg BP
    $354.00

    Gephyrin (B-4): m-IgGκ BP-HRP 套装

    sc-534224
    200 µg Ab; 40 µg BP
    $354.00

    Gephyrin (B-4): m-IgG2b BP-HRP 套装

    sc-549852
    200 µg Ab; 10 µg BP
    $354.00

    For Western Blot, is it recommended to use denatured or non-denatured conditions with Gephyrin (B-4): sc-55469 monoclonal antibody?

    Asked by: Germaine
    Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
    Answered by: Technical Support
    Date published: 2017-02-27
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    Rated 5 out of 5 by from Satisfactory Western Blot data of GephyrinSatisfactory Western Blot data of Gephyrin expression in H4, 3611-RF and PC-12 whole cell lysates and rat brain tissue extract . -SCBT QC
    Date published: 2015-02-28
    Rated 5 out of 5 by from Good Western Blot data of Gephyrin expressionGood Western Blot data of Gephyrin expression in non-transfected and mouse Gephyrin transfected 293T whole cell lysates. -SCBT QC
    Date published: 2013-12-01
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    Gephyrin抗体 (B-4) is rated 5.0 out of 5 by 2.
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