Date published: 2025-9-7
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
GDNF Antibody (B-8) - Western Blotting - Image 4390
GDNF 抗体 (B-8): sc-13147. 人重组 GDNF 的 Western 印迹分析.
GDNF Antibody (B-8) - Western Blotting - Image 4390
GDNF 抗体 (B-8): sc-13147. 人重组 GDNF 的 Western 印迹分析.
GDNF 抗体 (B-8): sc-13147. 人重组 GDNF 的 Western 印迹分析.

GDNF 抗体 (B-8): sc-13147

4.6(12)
寫評論提問

说明书
  • GDNF 抗体 B-8 是小鼠单克隆 IgG1 κ，GDNF抗体, 在52篇文献中引用，规格为200 µg/ml
  • 针对氨基酸78-134的抗原，代表成熟GDNF，来源于human
  • GDNF 抗体 (B-8) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 GDNF
  • 抗GDNF抗体（B-8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取GDNF (B-8): sc-13147的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 GDNF 抗体 (B-8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与GDNF 抗体 (B-8) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

GDNF抗体（B-8）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过多种应用检测小鼠、大鼠和人类样本中的胶质细胞系衍生神经营养因子（GDNF），包括蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）。GDNF (B-8) 抗体有非结合型和多种结合型，如琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和各种 Alexa Fluor® 结合物。GDNF在中脑多巴胺能神经元的存活和分化中发挥着关键作用，而多巴胺能神经元对于运动控制至关重要，在帕金森病等疾病中会受到严重影响。GDNF主要通过位于靶细胞表面的GFRα1受体分泌并作用于神经元。这种相互作用对于神经元的健康至关重要，因为GDNF激活信号通路，促进细胞存活和生长。GDNF位于细胞外空间，能够与受体有效结合并发挥神经保护作用，因此GDNF是神经发育的关键因子，也是神经退行性疾病的潜在治疗靶点。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

GDNF 抗体 (B-8) 参考文献:

  1. 分离雄性生殖系干细胞；GDNF 的影响。  |  Hofmann, MC., et al. 2005. Dev Biol. 279: 114-24. PMID: 15708562
  2. Ret, GDNF 和 GFRalpha1 分子之间的直接相互作用揭示了有关功能性三蛋白复合物组装的新见解。  |  Amoresano, A., et al. 2005. Cell Signal. 17: 717-27. PMID: 15722196
  3. GDNF 对培养的腹侧间脑多巴胺能和非多巴胺能钙网蛋白表达神经元分化的影响  |  Schaller, B., et al. 2005. Brain Res. 1036: 163-72. PMID: 15725414
  4. GDNF和GFRalpha1可促进大脑皮层GABA能神经元的分化和切线迁移。  |  Pozas, E. and Ibáñez, CF. 2005. Neuron. 45: 701-13. PMID: 15748846
  5. GDNF 与成人中枢儿茶酚胺能神经元的保护。  |  Pascual, A., et al. 2011. J Mol Endocrinol. 46: R83-92. PMID: 21357726
  6. 转化生长因子-β。生长调节肽家族。  |  Miller, DA., et al. 1990. Ann N Y Acad Sci. 593: 208-17. PMID: 2375595
  7. 鉴定转化生长因子β型基因家族的另一个成员。  |  ten Dijke, P., et al. 1988. Proc Natl Acad Sci U S A. 85: 4715-9. PMID: 3164476
  8. 运动神经元中 GDNF 的合成、信号传导和逆行运输。  |  Cintrón-Colón, AF., et al. 2020. Cell Tissue Res. 382: 47-56. PMID: 32897420
  9. 基于各向异性支架的GDNF负载聚多巴胺纳米颗粒通过调节抑制性微环境促进脊髓修复  |  Ma, J., et al. 2023. Adv Healthc Mater. 12: e2202377. PMID: 36549669
  10. 人类转化生长因子-β互补 DNA 序列及在正常细胞和转化细胞中的表达。  |  Derynck, R., et al. Nature. 316: 701-5. PMID: 3861940
  11. 大鼠中枢神经系统克隆细胞系。  |  Schubert, D., et al. 1974. Nature. 249: 224-7. PMID: 4151463
  12. GDNF：中脑多巴胺能神经元的神经胶质细胞系衍生神经营养因子。  |  Lin, LF., et al. 1993. Science. 260: 1130-2. PMID: 8493557

订购信息

产品名称产品编号规格价格数量收藏夹

GDNF 抗体 (B-8)

sc-13147
200 µg/ml
RMB2377.00

GDNF (B-8): m-IgG Fc BP-HRP 套装

sc-526542
200 µg Ab; 10 µg BP
RMB2662.00

GDNF (B-8): m-IgG1 BP-HRP 套装

sc-531915
200 µg Ab; 20 µg BP
RMB2662.00

GDNF 抗体 (B-8) AC

sc-13147 AC
500 µg/ml, 25% agarose
RMB3129.00

GDNF 抗体 (B-8) HRP

sc-13147 HRP
200 µg/ml
RMB2377.00

GDNF 抗体 (B-8) FITC

sc-13147 FITC
200 µg/ml
RMB2482.00

GDNF 抗体 (B-8) PE

sc-13147 PE
200 µg/ml
RMB2580.00

GDNF 抗体 (B-8) Alexa Fluor® 488

sc-13147 AF488
200 µg/ml
RMB2685.00

GDNF 抗体 (B-8) Alexa Fluor® 546

sc-13147 AF546
200 µg/ml
RMB2685.00

GDNF 抗体 (B-8) Alexa Fluor® 594

sc-13147 AF594
200 µg/ml
RMB2685.00

GDNF 抗体 (B-8) Alexa Fluor® 647

sc-13147 AF647
200 µg/ml
RMB2685.00

GDNF 抗体 (B-8) Alexa Fluor® 680

sc-13147 AF680
200 µg/ml
RMB2685.00

GDNF 抗体 (B-8) Alexa Fluor® 790

sc-13147 AF790
200 µg/ml
RMB2685.00

GDNF抗体(B-8): sc-13147　の吸収試験のためのペプチドは販売していますか？

Asked by: ひろちん
Thank you for your question. We are sorry. We do not sell that. ご質問ありがとうございます。申し訳ございません。それは販売しておりません。
Answered by: Jiajun Z
Date published: 2025-03-05

Can we use sc-13147 for IF and WB to detect GDNF in rat hippocampus (using either brain slices or brain tissue)? If yes, are there any published papers on this using this antibody? I couldn't find any. Thanks!

Asked by: Jenn12
Thank you for your question. We have not tested this antibody for rat hippocampus. We validate this antibody for mouse, rat and human samples .
Answered by: BlakeJ
Date published: 2023-02-23

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 13
Date published: 2017-01-17
  • y_2025, m_8, d_31, h_8CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_3
  • loc_zh_CN, sid_13147, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 103ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from GDNFIt works well.But the MW is at 24 KDa, not at 19 KDa as the datasheet says.
Date published: 2018-02-18
Rated 5 out of 5 by from It works well.It works well. The MW is at 24 KDa, not at 15 KDa as the datasheet says.
Date published: 2017-11-02
Rated 3 out of 5 by from It is badIt does not work in immunohistochemistry even diluted at 1:15.
Date published: 2017-10-24
Rated 5 out of 5 by from EXCELLENT!I applied for a sample one month ago and used it 2 times. I was so ecxited that the band was very clear, so was the background.
Date published: 2017-05-28
Rated 5 out of 5 by from Great featuresI bought this two month ago and so happy that I did Western blot (dilution 1:100), the results are mazing
Date published: 2017-05-25
Rated 5 out of 5 by from Best GDNF antibody for WB and IHCGDNF (B-8) is very well cited in literature for WB and IHC(P).
Date published: 2017-01-23
Rated 5 out of 5 by from Outstanding !!Superb product for both western blot and IHC applications
Date published: 2017-01-23
Rated 5 out of 5 by from It works wellWe observed specific 15 kDa band of GDNF in WB. Dilution of antibody is 1: 500. Samples are rat cells.
Date published: 2016-12-17
  • y_2025, m_8, d_31, h_8
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_12
  • loc_zh_CN, sid_13147, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 9ms
  • REVIEWS, PRODUCT
GDNF 抗体 (B-8) is rated 4.6 out of 5 by 12.
  • y_2025, m_8, d_31, h_8
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_12
  • loc_zh_CN, sid_13147, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 100ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。