Date published: 2025-9-7

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GDF-9B 抗体 (F-7): sc-271824

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说明书
  • GDF-9B 抗体 F-7 是小鼠单克隆 IgG1 κ,GDF-9B抗体, 在2篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列268-350位于human物种的GDF-9B的C-terminus附近
  • GDF-9B 抗体 (F-7) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测human 和hamster 来源的 mature and precursor GDF-9B
  • 抗GDF-9B抗体(F-7)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取GDF-9B (F-7): sc-271824免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgGκ BP-HRP 是 GDF-9B 抗体 (F-7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与GDF-9B 抗体 (F-7) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

GDF-9B抗体(F-7)是一种IgG1κ型小鼠单克隆GDF-9B抗体(也称为BMP15抗体或GDF9B抗体),通过WB、IP、IF、IHC(P)和ELISA检测来自人类和仓鼠的GDF-9B蛋白。GDF-9B抗体(F-7)既可以是非偶联的抗GDF-9B抗体形式,也可以是多种偶联形式的抗GDF-9B抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。生长/分化因子(GDFs)是TGF超家族的成员。TGF超家族成员参与胚胎发育和成人组织稳态。生长和分化因子9B(GDF-9B),也称为骨形态发生蛋白15(BMP15),仅在卵母细胞中表达。GDF-9B与另一种卵母细胞特异性成员GDF-9密切相关,后者已被证明对早期卵巢卵泡发生至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

GDF-9B 抗体 (F-7) 参考文献:

  1. 人类生长分化因子 9(GDF-9)及其新型同源物 GDF-9B 在卵母细胞早期卵泡生成过程中表达。  |  Aaltonen, J., et al. 1999. J Clin Endocrinol Metab. 84: 2744-50. PMID: 10443672
  2. 卵母细胞生长因子基因(BMP15)的突变以剂量敏感的方式导致排卵率增加和不孕症。  |  Galloway, SM., et al. 2000. Nat Genet. 25: 279-83. PMID: 10888873
  3. 卵巢早衰和多囊卵巢综合征患者的生长分化因子-9 和-9B 基因突变分析。  |  Takebayashi, K., et al. 2000. Fertil Steril. 74: 976-9. PMID: 11056243
  4. Bmp15 基因突变与卵巢功能  |  Galloway, SM., et al. 2002. Mol Cell Endocrinol. 191: 15-8. PMID: 12044914
  5. 转化生长因子-β 家族。  |  Massagué, J. 1990. Annu Rev Cell Biol. 6: 597-641. PMID: 2177343
  6. 一种新的 TGF-beta 超家族成员对小鼠骨骼肌质量的调节。  |  McPherron, AC., et al. 1997. Nature. 387: 83-90. PMID: 9139826
  7. 在卵泡生成过程中,一种新型生长分化因子-9(GDF-9)相关因子与 GDF-9 在小鼠卵母细胞中共同表达。  |  Laitinen, M., et al. 1998. Mech Dev. 78: 135-40. PMID: 9858711

订购信息

产品名称产品编号规格价格数量收藏夹

GDF-9B 抗体 (F-7)

sc-271824
200 µg/ml
RMB2377.00

GDF-9B (F-7): m-IgG Fc BP-HRP 套装

sc-529267
200 µg Ab; 10 µg BP
RMB2662.00

GDF-9B (F-7): m-IgGκ BP-HRP 套装

sc-522071
200 µg Ab, 40 µg BP
RMB2662.00

GDF-9B 抗体 (F-7) AC

sc-271824 AC
500 µg/ml, 25% agarose
RMB3129.00

GDF-9B 抗体 (F-7) HRP

sc-271824 HRP
200 µg/ml
RMB2377.00

GDF-9B 抗体 (F-7) FITC

sc-271824 FITC
200 µg/ml
RMB2482.00

GDF-9B 抗体 (F-7) PE

sc-271824 PE
200 µg/ml
RMB2580.00

GDF-9B 抗体 (F-7) Alexa Fluor® 488

sc-271824 AF488
200 µg/ml
RMB2685.00

GDF-9B 抗体 (F-7) Alexa Fluor® 546

sc-271824 AF546
200 µg/ml
RMB2685.00

GDF-9B 抗体 (F-7) Alexa Fluor® 594

sc-271824 AF594
200 µg/ml
RMB2685.00

GDF-9B 抗体 (F-7) Alexa Fluor® 647

sc-271824 AF647
200 µg/ml
RMB2685.00

GDF-9B 抗体 (F-7) Alexa Fluor® 680

sc-271824 AF680
200 µg/ml
RMB2685.00

GDF-9B 抗体 (F-7) Alexa Fluor® 790

sc-271824 AF790
200 µg/ml
RMB2685.00

We have done the experiment in cat ovary lysate in RIPA buffer and found that there are non-specific bands on the membrane. We would like to know whether this antibody works well and specific with BMP-15 in cat tissue or not? Do you have any suggestion?

Asked by: PROMPORN
Thank you for your question. Anti-GDF-9B Antibody (F-7) is only recommended for detection of mature and precursor GDF-9B of human and hamster origin by WB, IP, IF, IHC(P) and ELISA. It has not been tested in feline cell or tissue samples and is not recommended for such uses.
Answered by: Tech Support Europe
Date published: 2022-10-18

请问,这个牛上能做吗 GDF9B

Asked by: GDF9B
Thank you for your inquiry. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website. 感谢您的提问。请将您的目的蛋白序列发送到我们的技术支持部门。我们将对比您的序列以及我们的数据,以解答您物种反应性的相关问题。亚洲技术支持电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”。
Answered by: Technical Support 9
Date published: 2019-10-08

I am using GDF-9B (F-7): sc-271824 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: cjMara
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Robust signal in WB in HLRobust signal in WB in HL-60 whole cell lysate. -SCBT QC
Date published: 2015-05-11
Rated 5 out of 5 by from Great immunoperoxidase cytoplasmic stainingGreat immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human ovary tissue. -SCBT QC
Date published: 2015-03-19
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GDF-9B 抗体 (F-7) is rated 5.0 out of 5 by 2.
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