Date published: 2025-9-7
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GCK Antibody (G-6) - Immunofluorescence - Image 134177
GCK 抗体 (G-6): sc-17819. 甲醇固定的 HeLa 细胞免疫荧光染色, 显示胞质定位.
GCK 抗体 (G-6): sc-17819. 甲醇固定的 HeLa 细胞免疫荧光染色, 显示胞质定位.

GCK 抗体 (G-6): sc-17819

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说明书
  • GCK 抗体 G-6 是小鼠单克隆 IgG1 κ，GCK抗体, 在18篇文献中引用，规格为200 µg/ml
  • 免疫human物种的GCK的氨基酸318-405
  • GCK 抗体 (G-6) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 GCK
  • 抗GCK抗体（G-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取GCK (G-6): sc-17819的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是GCK Antibody (G-6) for WB applications. 的首选辅助检测试剂，这些试剂现与GCK Antibody (G-6) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

GCK抗体（G-6）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的GCK蛋白。抗GCK抗体（G-6）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。葡萄糖激酶（又称六碳糖激酶IV）在葡萄糖代谢中起着至关重要的作用，主要存在于胰腺β细胞和肝脏中。在胰腺β细胞中，葡萄糖激酶充当葡萄糖传感器，根据血糖水平调节胰岛素分泌，这对于维持血糖平衡至关重要。在肝脏中，葡萄糖激酶催化葡萄糖的磷酸化，这是葡萄糖处理的第一步。这种酶活性至关重要，因为它决定了葡萄糖代谢的速度，而GCK的任何功能障碍都会导致严重的代谢紊乱。例如，GCK基因突变与青少年发病型糖尿病（MODY）有关，这凸显了这种蛋白质在胰岛素调节和葡萄糖代谢中的重要性。GCK单克隆抗体（G-6）能够有效检测和定量GCK，因此这种抗体成为糖尿病和代谢性疾病研究的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

GCK 抗体 (G-6) 参考文献:

  1. 关于葡萄糖激酶（GCK）突变的最新进展，这种突变可导致成熟期发病的幼年糖尿病、永久性新生儿糖尿病和高胰岛素血症性低血糖。  |  Osbak, KK., et al. 2009. Hum Mutat. 30: 1512-26. PMID: 19790256
  2. 在临床非选择性队列中，MODY 相关 HNF1A/HNF4A 变体的穿透性降低，但 GCK 变体的穿透性并不降低。  |  Mirshahi, UL., et al. 2022. Am J Hum Genet. 109: 2018-2028. PMID: 36257325
  3. 在缺血再灌注损伤中抑制 Hmbox1 可通过 Gck 激活促进心肌细胞存活和葡萄糖代谢  |  Bei, Y., et al. 2024. Circulation. 150: 848-866. PMID: 38708602
  4. 糖尿病中的葡萄糖激酶（GCK）：从分子机制到疾病发病机制。  |  Abu Aqel, Y., et al. 2024. Cell Mol Biol Lett. 29: 120. PMID: 39245718
  5. 转基因基因敲除揭示了胰腺β细胞葡萄糖激酶在维持葡萄糖稳态中的关键作用。  |  Grupe, A., et al. 1995. Cell. 83: 69-78. PMID: 7553875
  6. 糖尿病 90% 胰腺切除大鼠离体胰岛中的己糖激酶活性上调。  |  Hosokawa, H., et al. 1995. Diabetes. 44: 1328-33. PMID: 7589832
  7. 人类和大鼠 beta 细胞的葡萄糖转运体基因表达不同，但葡萄糖激酶基因表达没有差异。  |  De Vos, A., et al. 1995. J Clin Invest. 96: 2489-95. PMID: 7593639
  8. 成熟-发病-青年糖尿病（MODY）缔合作用的葡萄糖激酶突变对底物相互作用和酶稳定性的不同影响。  |  Liang, Y., et al. 1995. Biochem J. 309 (Pt 1): 167-73. PMID: 7619052
  9. 通过破坏小鼠葡萄糖激酶基因产生的成熟期青少年糖尿病动物模型。  |  Bali, D., et al. 1995. J Biol Chem. 270: 21464-7. PMID: 7665557
  10. 葡萄糖调节未成熟胎鼠胰岛中葡萄糖激酶的最大速度和葡萄糖的利用。  |  Tu, J. and Tuch, BE. 1996. Diabetes. 45: 1068-75. PMID: 8690154
  11. 葡萄糖传感器蛋白葡萄糖激酶在分泌胰高血糖素的α细胞中表达。  |  Heimberg, H., et al. 1996. Proc Natl Acad Sci U S A. 93: 7036-41. PMID: 8692940
  12. 通过葡萄糖激酶纠正糖尿病改变。  |  Ferre, T., et al. 1996. Proc Natl Acad Sci U S A. 93: 7225-30. PMID: 8692973

订购信息

产品名称产品编号规格价格数量收藏夹

GCK 抗体 (G-6)

sc-17819
200 µg/ml
RMB2377.00

GCK (G-6): m-IgG Fc BP-HRP 套装

sc-526575
200 µg Ab; 10 µg BP
RMB2662.00

GCK (G-6): m-IgG1 BP-HRP 套装

sc-531948
200 µg Ab; 20 µg BP
RMB2662.00

GCK 抗体 (G-6) AC

sc-17819 AC
500 µg/ml, 25% agarose
RMB3129.00

GCK 抗体 (G-6) HRP

sc-17819 HRP
200 µg/ml
RMB2377.00

GCK 抗体 (G-6) FITC

sc-17819 FITC
200 µg/ml
RMB2482.00

GCK 抗体 (G-6) PE

sc-17819 PE
200 µg/ml
RMB2580.00

GCK 抗体 (G-6) Alexa Fluor® 488

sc-17819 AF488
200 µg/ml
RMB2685.00

GCK 抗体 (G-6) Alexa Fluor® 546

sc-17819 AF546
200 µg/ml
RMB2685.00

GCK 抗体 (G-6) Alexa Fluor® 594

sc-17819 AF594
200 µg/ml
RMB2685.00

GCK 抗体 (G-6) Alexa Fluor® 647

sc-17819 AF647
200 µg/ml
RMB2685.00

GCK 抗体 (G-6) Alexa Fluor® 680

sc-17819 AF680
200 µg/ml
RMB2685.00

GCK 抗体 (G-6) Alexa Fluor® 790

sc-17819 AF790
200 µg/ml
RMB2685.00

I have two questions about this antibody.1)The two cell lines that you show (HeLa and Mia PACA) were transfected with glucokinase or this is an endogenous expression? 2) is HepG2 a positive control?

Asked by: Andres355
Thank you for your question. The immunofluorescence data for this antiobody is in endogenous samples. HepG2 is one of the recommended positive controls for this protein.
Answered by: Tech Service
Date published: 2019-02-18

I wonder whether this antibody has ever been used to detect GCK in tumor tissues other than liver. Do you have any evidence that this protein is expressed also in cancers other than hepatocarcinomas?

Asked by: vicol
Thanks for your question. We have not performed biological studies directly. However there are two pubblications about this antibody: PMID 27802600 and PMID 29609037. About the protein you can find useful information in this link: https://www.proteinatlas.org/ENSG00000106633-GCK/antibody If you have any further questions, please contact Technical Service.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

GCK (glucokinase, NP_000153.1) does not expresses in HepG2. Besides, GCK MW=52kDa and in the band shown in the wb migrates quite higher. Has the identity of the shown band been verified by sequencing?.

Asked by: maykel
Thank you for your question. There is literature supporting expression of GCK in liver. If you would like to share with us any literature reference supporting a lack of expression of GCK in the HepG2 cell line we are happy to review the reference and reevaluate our data. We have not verified the identity of the bands detected in Western with this antibody through sequencing. Using this mouse monoclonal antibody, GCK (G-6): sc-17819, we have observed GCK at a molecular weight higher than the predicted molecular weight of 52 kDa in multiple cell lines. This difference may be due to post-translational modifications of the protein, or other factors which would influence the observed molecular weight of the protein in an SDS-PAGE gel.
Answered by: Technical Support
Date published: 2017-08-16

What is the recommended fixation method for immunofluorescence staining with GCK (G-6): sc-17819 monoclonal antibody?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2022-07-18
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Rated 5 out of 5 by from Successful detection of GCK in HepG2 cellsThe GCK antibody works well for detection of GCK in HepG2 cells. The antibody is very clean.
Date published: 2021-06-24
Rated 5 out of 5 by from Great detection!Great signal from rat hypothalamic proteins. Single band using the antibody 1: 2000
Date published: 2018-08-10
Rated 4 out of 5 by from Two bandsPerformed immunoblotting with this antibody at 1:500 in MEF whole cell lysates with RIPA. I saw a band at around 50 kD and another at around 80-90 kD. The sample pictures shown for this antibody looks like the band is between 55 and 90 kD, while the predicted band size from the specification sheet says 50 kD. So its confusing as to which band should be used.
Date published: 2017-09-21
Rated 5 out of 5 by from Great for co-IP.Hign efficiency for endogenous co-IP and single western bolt band.
Date published: 2017-09-08
Rated 5 out of 5 by from Clear bandThe antibody diluted 1/500 for WB. Nice single band at around 50 kDa,the detected sample loading about 60ug.
Date published: 2017-08-26
Rated 5 out of 5 by from Good resultThis antibody is used for evaluating activity of the GCK after stimulation or inhibition of GCK in lung cancer cell lines. The antibody is very reliable. We have found that dilution in 5 % BSA is optimal .Dilution: 1:500 is good choice dilution.
Date published: 2017-08-12
Rated 5 out of 5 by from GoodWestern was done in mouse liver lysate with 50ug protein input, 1:1000 primary antibody O/N, the band was very clear.
Date published: 2017-07-20
Rated 3 out of 5 by from Worked well with transfected lysate by WBWorked well with transfected lysate by WB -SCBT QC
Date published: 2015-03-16
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GCK 抗体 (G-6) is rated 4.5 out of 5 by 10.
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