Date published: 2025-9-6
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GATA3 Antibody (HG3-35) - Western Blotting - Image 1178
GATA-3 抗体 (HG3-35): sc-269. MOLT-4 核提取物 (A) 和 CCRF-HSB-2 全细胞裂解液 (B) 中 GATA-3 表达的 Western 印迹分析.
GATA-3 抗体 (HG3-35): sc-269. MOLT-4 核提取物 (A) 和 CCRF-HSB-2 全细胞裂解液 (B) 中 GATA-3 表达的 Western 印迹分析.

GATA3 抗体 (HG3-35): sc-269

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说明书
  • GATA3抗体(HG3-35)是小鼠单克隆IgG1 κ, 在49篇文献中引用，规格为200 µg/ml
  • 免疫human物种的GATA-3的氨基酸1-264
  • GATA3 抗体 (HG3-35) 推荐用于WB, IP, IF 和 IHC(P)，检测human 来源的 GATA-3
  • 抗GATA3抗体（HG3-35）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-269 X, 200 µg/0.1 ml)
  • 关于如何获取GATA3 (HG3-35): sc-269的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 GATA3 抗体 (HG3-35) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与GATA3 抗体 (HG3-35) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 GATA 抗体 包括 GATA-1, GATA-2, GATA-3, GATA-4, GATA-5和GATA-6

GATA-3抗体（HG3-35）是一种鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学（IHC）检测人类来源的GATA-3。GATA-3 单克隆抗体（HG3-35）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种 Alexa Fluor® 结合物。 GATA-3 在 T 淋巴细胞的发展和功能中起着至关重要的作用，它是一种关键的转录因子，可调节 T 细胞分化与功能相关基因的表达。GATA-3的表达对于免疫系统的发育至关重要，尤其是辅助性T细胞的分化，而辅助性T细胞对于协调免疫反应至关重要。GATA-3主要位于细胞核中，与特定的DNA序列结合以调节基因表达，这凸显了GATA-3在细胞信号通路中的重要性。GATA-3已知与其他转录因子相互作用，例如T-bet和RORγt，这进一步强调了GATA-3在控制T细胞谱系承诺和功能的复杂调节网络中的作用。了解GATA-3的动态和相互作用对于阐明免疫反应的潜在治疗靶点和免疫相关疾病的潜在治疗靶点至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

GATA3 抗体 (HG3-35)

sc-269
200 µg/ml
RMB2377.00

GATA3 (HG3-35): m-IgG Fc BP-HRP 套装

sc-526454
200 µg Ab; 10 µg BP
RMB2662.00

GATA3 (HG3-35): m-IgG1 BP-HRP 套装

sc-531827
200 µg Ab; 20 µg BP
RMB2662.00

GATA3 抗体 (HG3-35) X

sc-269 X
200 µg/0.1 ml
RMB2377.00

GATA3 抗体 (HG3-35) AC

sc-269 AC
500 µg/ml, 25% agarose
RMB3129.00

GATA3 抗体 (HG3-35) HRP

sc-269 HRP
200 µg/ml
RMB2377.00

GATA3 抗体 (HG3-35) FITC

sc-269 FITC
200 µg/ml
RMB2482.00

GATA3 抗体 (HG3-35) PE

sc-269 PE
200 µg/ml
RMB2580.00

GATA3 抗体 (HG3-35) Alexa Fluor® 488

sc-269 AF488
200 µg/ml
RMB2685.00

GATA3 抗体 (HG3-35) Alexa Fluor® 546

sc-269 AF546
200 µg/ml
RMB2685.00

GATA3 抗体 (HG3-35) Alexa Fluor® 594

sc-269 AF594
200 µg/ml
RMB2685.00

GATA3 抗体 (HG3-35) Alexa Fluor® 647

sc-269 AF647
200 µg/ml
RMB2685.00

GATA3 抗体 (HG3-35) Alexa Fluor® 680

sc-269 AF680
200 µg/ml
RMB2685.00

GATA3 抗体 (HG3-35) Alexa Fluor® 790

sc-269 AF790
200 µg/ml
RMB2685.00

What is the difference between HG3-31 and HG3-35?

Asked by: Vincent YU
Thank you for your question. These are two different antibody clones directed to detect GATA-3. However, they do target different epitopes, and have different reactivities. For example, sc-268 (HG3-31) is mouse, rat and human reactive, while sc-269 (HG3-35) is human reactive only.
Answered by: Tech Service
Date published: 2019-12-09

The GATA-3 antibody (HG3-31) works OK in MCF-7 cells, but it has high background in WB with JEG-3 cells. May I have free sample of HG3-35 to test with JEG-3 cells?

Asked by: monhu
Thank you for your question. Please contact Technical Service or your local distributor for information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Tech Service
Date published: 2019-05-24

When should the TransCruz reagent sc-269 X be used instead of the standard form sc-269?

Asked by: cjMara
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from GATA3 antibody works well for ChIP-qPCR in humanThis GATA3 antibody works well for ChIP-qPCR in human cell lines. https://www.pnas.org/doi/10.1073/pnas.1602886113
Date published: 2025-02-16
Rated 4 out of 5 by from GATA-3Works well in IHC -FFPE. very clean and intense staining observed.
Date published: 2018-04-20
Rated 5 out of 5 by from Imunofluorescência Excelente!Pedi uma amostra grátis para testar em imunoflourescência e funcionou bem!
Date published: 2018-03-07
Rated 5 out of 5 by from Works well in FFPEGreat choice when working with paraffin-embedded sections in IHC. Antigen retrieval recommended.
Date published: 2017-01-24
Rated 5 out of 5 by from Works well in WB with mouse spleen cellsWorks well in WB with mouse spleen cells -SCBT Publication Review
Date published: 2015-02-15
Rated 5 out of 5 by from Good for ChIP with Jurkat cell lysatesGood for ChIP with Jurkat cell lysates -SCBT Publication Review
Date published: 2015-02-02
Rated 5 out of 5 by from Low background and strong bands in WB with MOLTLow background and strong bands in WB with MOLT-4 nuclear extract and CCRF-HSB-2 whole cell lysate. -SCBT QC
Date published: 2013-02-23
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GATA3 抗体 (HG3-35) is rated 4.9 out of 5 by 7.
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