Date published: 2025-9-6
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GATA3 Antibody (HG3-31) - Western Blotting - Image 1175
GATA-3 抗体 (HG3-31): sc-268. Jurkat (A), CCRF-HSB-2 (B) 和 MOLT-4 (C) 全细胞裂解液中 GATA-3 表达的 Western 印迹分析.
GATA-3 抗体 (HG3-31): sc-268. Jurkat (A), CCRF-HSB-2 (B) 和 MOLT-4 (C) 全细胞裂解液中 GATA-3 表达的 Western 印迹分析.

GATA3 抗体 (HG3-31): sc-268

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说明书
  • GATA3 抗体 HG3-31 是小鼠单克隆 IgG1 κ，GATA3抗体, 在425篇文献中引用，规格为200 µg/ml
  • 免疫human物种的重组GATA-3
  • GATA3 抗体 (HG3-31) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat, human 和avian 来源的 GATA-3
  • 抗GATA3抗体（HG3-31）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-268 X, 200 µg/0.1 ml)
  • 关于如何获取GATA3 (HG3-31): sc-268的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 GATA3 抗体 (HG3-31) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与GATA3 抗体 (HG3-31) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 GATA 抗体 包括 GATA-1, GATA-2, GATA-3, GATA-4, GATA-5和GATA-6

GATA-3抗体（HG3-31）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学（IHC）检测小鼠、大鼠、人类和鸟类来源的GATA-3蛋白。GATA-3 单克隆抗体（HG3-31）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种 Alexa Fluor® 结合型。 GATA-3 在 T 淋巴细胞的发展和功能中起着至关重要的作用，它作为一种转录因子，可调节 T 细胞分化与功能所必需的基因的表达。GATA-3在免疫反应中尤其重要，因为GATA-3参与Th2细胞的发展，而Th2细胞对于协调细胞外病原体反应和过敏反应至关重要。GATA-3主要位于T细胞的细胞核中，在那里GATA-3与特定的DNA序列结合以调节基因表达。核定位对于功能至关重要，因为对靶基因转录的直接影响会驱动T细胞的发展和功能。GATA-3对这些过程的调节在正常免疫功能和包括过敏和自身免疫性疾病在内的各种疾病的发病机理中均具有重要意义。GATA-3单克隆抗体（HG3-31）为研究T细胞生物学和免疫反应的潜在机制的研究人员提供了强有力的工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

GATA3 抗体 (HG3-31) 参考文献:

  1. 人类转录因子 GATA-2。内皮细胞中前内皮素-1 基因表达调控的证据。  |  Dorfman, DM., et al. 1992. J Biol Chem. 267: 1279-85. PMID: 1370462
  2. CTLA-4 通过调节每个细胞的 GATA-3 蛋白水平来调节过敏原反应。  |  Nasta, F., et al. 2007. Immunology. 121: 62-70. PMID: 17313444
  3. GATA-3--不再只适用于 Th2 细胞。  |  Ho, IC. and Pai, SY. 2007. Cell Mol Immunol. 4: 15-29. PMID: 17349208
  4. Gata-3 与乳腺细胞的命运  |  Naylor, MJ. and Ormandy, CJ. 2007. Breast Cancer Res. 9: 302. PMID: 17381824
  5. 4-1BB 触发 T 细胞产生 IL-13，以限制由 Th1 介导的极化炎症。  |  Shin, SM., et al. 2007. J Leukoc Biol. 81: 1455-65. PMID: 17389581
  6. 小鼠和人类 T 淋巴细胞 GATA-3 因子通过人类 T 细胞受体 delta 基因增强子中的顺式调节元件介导转录。  |  Ko, LJ., et al. 1991. Mol Cell Biol. 11: 2778-84. PMID: 2017177
  7. GATA-4/5/6，在发育中的心脏和肠道中转录的三个转录因子亚家族。  |  Laverriere, AC., et al. 1994. J Biol Chem. 269: 23177-84. PMID: 8083222
  8. GATA 转录因子家族的 DNA 结合特异性。  |  Ko, LJ. and Engel, JD. 1993. Mol Cell Biol. 13: 4011-22. PMID: 8321208
  9. 人类 GATA-6 基因：结构, 染色体位置以及组织特异性和有丝分裂原反应信号对表达的调控。  |  Suzuki, E., et al. 1996. Genomics. 38: 283-90. PMID: 8975704

订购信息

产品名称产品编号规格价格数量收藏夹

GATA3 抗体 (HG3-31)

sc-268
200 µg/ml
RMB2377.00

GATA3 (HG3-31): m-IgG Fc BP-HRP 套装

sc-528106
200 µg Ab; 10 µg BP
RMB2662.00

GATA3 (HG3-31): m-IgGκ BP-HRP 套装

sc-520404
200 µg Ab, 40 µg BP
RMB2662.00

GATA3 (HG3-31): m-IgG1 BP-HRP 套装

sc-542762
200 µg Ab; 20 µg BP
RMB2662.00

GATA3 抗体 (HG3-31) X

sc-268 X
200 µg/0.1 ml
RMB2377.00

GATA3 抗体 (HG3-31) AC

sc-268 AC
500 µg/ml, 25% agarose
RMB3129.00

GATA3 抗体 (HG3-31) HRP

sc-268 HRP
200 µg/ml
RMB2377.00

GATA3 抗体 (HG3-31) FITC

sc-268 FITC
200 µg/ml
RMB2482.00

GATA3 抗体 (HG3-31) PE

sc-268 PE
200 µg/ml
RMB2580.00

GATA3 抗体 (HG3-31) Alexa Fluor® 488

sc-268 AF488
200 µg/ml
RMB2685.00

GATA3 抗体 (HG3-31) Alexa Fluor® 546

sc-268 AF546
200 µg/ml
RMB2685.00

GATA3 抗体 (HG3-31) Alexa Fluor® 594

sc-268 AF594
200 µg/ml
RMB2685.00

GATA3 抗体 (HG3-31) Alexa Fluor® 647

sc-268 AF647
200 µg/ml
RMB2685.00

GATA3 抗体 (HG3-31) Alexa Fluor® 680

sc-268 AF680
200 µg/ml
RMB2685.00

GATA3 抗体 (HG3-31) Alexa Fluor® 790

sc-268 AF790
200 µg/ml
RMB2685.00

I have searched for good GATA3 antibody for liver tissues. I have tested several GATA3 antibody, the signals are not good. Does this antibody can meet my requirement?

Asked by: JUBOUWANG
Thank you for contacting Santa Cruz Biotechnology. GATA-3 (HG3-31) antibody has shown to work for liver please see pubmed IDs. We also have an antibody sample program for more information please email [email protected]. 19922796 36109558
Answered by: BlakeJ
Date published: 2024-11-22

I used this antigen in IF staining, but it did not work. I used heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). Should I change this buffer?

Asked by: TS10
Thank you for your question.Our recommended conditions for antigen retrieval are: 10 mM sodium citrate buffer, pH 6.0 (sc-294091); or with 50 mM glycine-HCl buffer (sc-29096), pH 3.5, with 0.01% (w/v) EDTA (sc-29092). Heat at 95° C for 5 minutes.Contact your dealer if you have any other questions.Thank you.
Answered by: Jiajun Z
Date published: 2023-11-20

Does it work on immunofluorescence of FFPE tissues (e.g. human lung and liver tissue)?

Asked by: rmmphd
Thank you for your question. Anti-GATA-3 Antibody (HG3-31) is recommended for detection of GATA-3 of mouse, rat, human and avian origin by WB, IP, IF and IHC(P) and will also work on FFPE-treated human lung and liver tissue samples.
Answered by: Tech Support Europe
Date published: 2022-07-12

What is the region of the amino acids (i.e. N-terminus or C-terminus?) in the recombinant human GATA-3 raised for this monoclonal antibody?

Asked by: Jay1980
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext.1, allowing for a more interactive discussion of this and other related questions.
Answered by: Blake J
Date published: 2021-11-30

We bought this antibody, GATA-3 Antibody (HG3-31): sc-268, and did IF on the sections ( 5 um) of mouse breast tumors after antigen unmasking (PH 6, pressure cooking 2 min). However, the location of GATA3 is in the cytoplasm, not in the nuclei. Why?

Asked by: Mmcc
Thank you for your question. Please contact our Technical Service Department for help with troubleshooting the antibody. Please call us toll-free at (800) 457-3801 option 2, or use the live chat on our website.
Answered by: Technical Support
Date published: 2018-11-22

Does the HG3-31 antibody show significant cross-reactivity with the other GATA factors (that is GATA-1, GATA-2, GATA-4, GATA-5 or GATA-6)?

Asked by: wliam
Thank you for your question. This mouse monoclonal, GATA-3 Antibody (HG3-31): sc-268, is specific for GATA-3 and does not cross-react with other GATA factors. The antibody is extremely well characterized, and has been cited in the literature over 300 times. If you would be interested in testing a 10 µg sample of this antibody, for a limited time, we are offering a sample size. Please contact our Technical Service Department either by phone at (800) 457-3801 option 2, email [email protected], or use our live chat!
Answered by: Technical Support
Date published: 2018-09-10

may i ask that this antibody can detect GATA3 in pig?

Asked by: leseichi
Thank you for your question. We have not tested our mouse monoclonal, GATA-3 Antibody (HG3-31): sc-268, in pig. However, the antibody is raised against a recombinant protein immunogen that shares 94% sequence identity with the published protein for GATA-3 in pig (NP_001038032). Based on this sequence identity, we would expect this antibody to cross-react with GATA-3 in pig. If you are interested in testing a free sample of this antibody, please contact our Technical Service Department. As part of a limited time promotion, we are offering a free 10µg sample of our mouse monoclonal, GATA-3 Antibody (HG3-31): sc-268. Please call our Asian Support office toll-free at 800-820-8626, email [email protected], or contact us by live chat.
Answered by: Technical Support
Date published: 2017-02-01

Dilution and protocol to use on Dako Autostainer Link 48? Thanks

Asked by: joann
Thank you for your question. Our recommended immunohistochemistry protocol for using our monoclonal, GATA-3 Antibody (HG3-31): sc-268, is provided below. We have not used this antibody in combination with the Dako Autostainer Link 48 system, so we are unable to provide you any specific recommendations. However, this antibody has been validated for IHC on formalin-fixed paraffin-embedded tissue sections so it should be compatible. You may want to contact the other supplier to see if they can offer any specific recommendations for antibody usage with their equipment. https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-01-25
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Rated 1 out of 5 by from immunofluorescenceI used it for IF at different concentration on human tissue and following all the suggestion but it didn't work.
Date published: 2024-08-23
Rated 1 out of 5 by from Not workingCannot get a specific band and got multiple unspecific bands.
Date published: 2020-10-08
Rated 1 out of 5 by from not workingI purchased this ab two weeks ago since was one of the best rated for GATA3. I tried BSA and MILK blocking, 1 to 200 dilution and increasing concentrations of MCF7 cell lysates. It did not work so far
Date published: 2018-10-29
Rated 4 out of 5 by from anti GATA3 sc-268anti GATA3 sc-268 , dilution 1/500 in human breast cancer cells
Date published: 2018-10-19
Rated 4 out of 5 by from Strong bandStrong band seen in WB using GATA-3 in whole cell lysate.
Date published: 2017-10-10
Rated 5 out of 5 by from GATA-3I used the antibody for immunofluroscence staining and it worked excellent
Date published: 2017-09-22
Rated 4 out of 5 by from GATA-3 wb is okayWB: 1:1000, mouse spleen tissue, 5% BSA block, antibody duluting with 5%BSA in TBST buffer. WB, two bind attached photos
Date published: 2017-09-02
Rated 4 out of 5 by from Good antibodyThe antibody works great for westernblotting using mouse brain tissue at 1:500 dilution.
Date published: 2017-07-24
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GATA3 抗体 (HG3-31) is rated 4.0 out of 5 by 24.
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