Date published: 2025-9-6
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GATA-2 Antibody (CG2-96) - Western Blotting - Image 8813
GATA-2 抗体 (CG2-96): sc-267. TtT-97 核提取物中 GATA-2 表达的 Western 印迹分析.
GATA-2 Antibody (CG2-96) - Western Blotting - Image 8813
GATA-2 抗体 (CG2-96): sc-267. TtT-97 核提取物中 GATA-2 表达的 Western 印迹分析.
GATA-2 Antibody (CG2-96) - Western Blotting - Image 57954
GATA-2 抗体 (CG2-96): sc-267. TtT-97 核提取物中 GATA-2 表达的 Western 印迹分析. 科罗拉多大学的 David Gordon 博士友情提供.
GATA-2 抗体 (CG2-96): sc-267. TtT-97 核提取物中 GATA-2 表达的 Western 印迹分析.

GATA-2 抗体 (CG2-96): sc-267

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寫評論提問

说明书
  • GATA-2抗体(CG2-96)是小鼠单克隆IgG1 κ, 在74篇文献中引用，规格为200 µg/ml
  • 针对GATA-2
  • 抗-GATA-2 抗体 (CG2-96) 推荐用于 WB, IP 和 IF，检测mouse, rat, human 和avian 来源的 GATA-2
  • 抗GATA-2抗体（CG2-96）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-267 X, 200 µg/0.1 ml)
  • 关于如何获取GATA-2 (CG2-96): sc-267的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是GATA-2 Antibody (CG2-96) for WB applications. 的首选辅助检测试剂，这些试剂现与GATA-2 Antibody (CG2-96) 打包提供（请参阅下面的订购信息）。

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相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

额外GATA抗体，包括GATA-1, GATA-2, GATA-3, GATA-4, GATA-5和GATA-6

GATA-2抗体（CG2-96）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）和免疫荧光（IF）检测小鼠、大鼠、人类和鸟类来源的GATA-2蛋白。GATA-2 单克隆抗体（CG2-96）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种 Alexa Fluor® 结合物。GATA-2 在造血过程中起着至关重要的作用，对于多种血系（包括红细胞和骨髓细胞）的发育必不可少。GATA-2在胚胎脑和肝组织以及内皮细胞和HeLa细胞中的表达尤为突出。GATA-2包含高度保守的锌指DNA结合结构域，可与特定DNA序列结合，从而调节基因表达。GATA-2已被证明能够与其他GATA家族成员（如GATA-1和GATA-3）相互作用，从而增强调节能力。这种相互作用表明，GATA-2可以调节细胞分化与发育相关基因的表达，因此GATA-2在各种生物过程中发挥着关键作用。了解GATA-2的功能和相互作用对于阐明其在正常生理学中的作用以及其在白血病和其他血液疾病中的意义至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

GATA-2 抗体 (CG2-96) 参考文献:

  1. 造血转录因子GATA-1和GATA-2在红细胞系发育中的作用。  |  Ohneda, K. and Yamamoto, M. 2002. Acta Haematol. 108: 237-45. PMID: 12432220
  2. 人类转录因子 GATA-2。内皮细胞中前内皮素-1 基因表达调控的证据。  |  Dorfman, DM., et al. 1992. J Biol Chem. 267: 1279-85. PMID: 1370462
  3. 同源造血调节因子 Lyl1 和 Scl 受 Ets 和 GATA 因子的核心调节，但 Lyl1 无法挽救 Scl-/- 的早期表型。  |  Chan, WY., et al. 2007. Blood. 109: 1908-16. PMID: 17053063
  4. 细胞因子和整合素刺激协同作用，促进骨髓中原代人类造血祖细胞中 GATA-2, c-myb 和 CD34 蛋白水平的提高。  |  Dao, MA. and Nolta, JA. 2007. Blood. 109: 2373-9. PMID: 17095623
  5. 情境依赖性 GATA 因子功能：造血细胞和内皮细胞转录控制的组合要求。  |  Wozniak, RJ., et al. 2007. J Biol Chem. 282: 14665-74. PMID: 17347142
  6. Gata2 的内含子增强子使其具有泛内皮细胞特异性调控功能。  |  Khandekar, M., et al. 2007. Development. 134: 1703-12. PMID: 17395646
  7. 小鼠和人类 T 淋巴细胞 GATA-3 因子通过人类 T 细胞受体 delta 基因增强子中的顺式调节元件介导转录。  |  Ko, LJ., et al. 1991. Mol Cell Biol. 11: 2778-84. PMID: 2017177
  8. GATA-2介导的正常造血干细胞/祖细胞功能、骨髓增生异常和骨髓性白血病的调控。  |  Rodrigues, NP., et al. 2012. Int J Biochem Cell Biol. 44: 457-60. PMID: 22192845
  9. GATA-2 通过与 Smad4 相互作用抑制转化生长因子-β 信号通路。  |  Dong, XM., et al. 2014. Cell Signal. 26: 1089-97. PMID: 24509415
  10. GATA-4/5/6，在发育中的心脏和肠道中转录的三个转录因子亚家族。  |  Laverriere, AC., et al. 1994. J Biol Chem. 269: 23177-84. PMID: 8083222
  11. GATA 转录因子家族的 DNA 结合特异性。  |  Ko, LJ. and Engel, JD. 1993. Mol Cell Biol. 13: 4011-22. PMID: 8321208
  12. 人类 GATA-6 基因：结构, 染色体位置以及组织特异性和有丝分裂原反应信号对表达的调控。  |  Suzuki, E., et al. 1996. Genomics. 38: 283-90. PMID: 8975704

订购信息

产品名称产品编号规格价格数量收藏夹

GATA-2 抗体 (CG2-96)

sc-267
200 µg/ml
RMB2377.00

GATA-2 (CG2-96): m-IgG Fc BP-HRP 套装

sc-526453
200 µg Ab; 10 µg BP
RMB2662.00

GATA-2 (CG2-96): m-IgG1 BP-HRP 套装

sc-531826
200 µg Ab; 20 µg BP
RMB2662.00

GATA-2 抗体 (CG2-96) X

sc-267 X
200 µg/0.1 ml
RMB2377.00

GATA-2 抗体 (CG2-96) AC

sc-267 AC
500 µg/ml, 25% agarose
RMB3129.00

GATA-2 抗体 (CG2-96) HRP

sc-267 HRP
200 µg/ml
RMB2377.00

GATA-2 抗体 (CG2-96) FITC

sc-267 FITC
200 µg/ml
RMB2482.00

GATA-2 抗体 (CG2-96) PE

sc-267 PE
200 µg/ml
RMB2580.00

GATA-2 抗体 (CG2-96) Alexa Fluor® 488

sc-267 AF488
200 µg/ml
RMB2685.00

GATA-2 抗体 (CG2-96) Alexa Fluor® 546

sc-267 AF546
200 µg/ml
RMB2685.00

GATA-2 抗体 (CG2-96) Alexa Fluor® 594

sc-267 AF594
200 µg/ml
RMB2685.00

GATA-2 抗体 (CG2-96) Alexa Fluor® 647

sc-267 AF647
200 µg/ml
RMB2685.00

GATA-2 抗体 (CG2-96) Alexa Fluor® 680

sc-267 AF680
200 µg/ml
RMB2685.00

GATA-2 抗体 (CG2-96) Alexa Fluor® 790

sc-267 AF790
200 µg/ml
RMB2685.00

Do you offer a small trial aliquot of this antibody?

Asked by: Martin Larke
Thank you for your question. Contact our Technical Service Department to arrange for a free 10 µg sample of our GATA-2 Antibody (CG2-96): sc-267.
Answered by: Tech Support Europe
Date published: 2019-06-04

When should the TransCruz reagent sc-267 X be used instead of the standard form sc-267?

Asked by: jenni
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Service 11
Date published: 2017-01-10
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Rated 5 out of 5 by from GATA2 antibody works well for ChIP-qPCR in humanThis GATA2 antibody (mouse IgG monoclonal) worked well at the suggested concentrations for chomatin immunoprecipitation (ChIP-qPCR) in human cell lines. https://www.pnas.org/doi/10.1073/pnas.1602886113
Date published: 2025-02-13
Rated 4 out of 5 by from Good antibodyI used GATA-2 for WB, the antibody diluted at 1:500 and incubated ON. I suggest use the secondary at dilution 1:20000 for 90min. I obtained good signal with little background noise.
Date published: 2021-04-29
Rated 5 out of 5 by from satisfied resultWe choose this antibody because it can gave us re-producable western blot result. We have been using it for years and work best compared to other brands.Our purpose is to create publishable data and this antibody is being used detect the GATA-2 expression in rat kidney tissue. GATA-2 was used to ensure that our CRISPR knockout worked and was validated using the antibody.It gave us saisfied result.
Date published: 2017-07-29
Rated 5 out of 5 by from nice qualityWe obtained best result for GATA-2 by WB,the antibody diluted at 1:600 and incubated 2hour at RT.We suggest use the secondary at dilution 1:5000.
Date published: 2017-07-02
Rated 5 out of 5 by from WB analysisApplied in WB to detect human lung cancer cells, dilution is 1:500, shows single and clear band.
Date published: 2017-05-13
Rated 5 out of 5 by from Strong western !!Nice western blot data; strong signal and low background
Date published: 2017-01-14
Rated 5 out of 5 by from Strong Western blot signalThe GATA-2 Antibody (CG2-96) works very well for Western blot showing a strong signal with little to no background noise. This antibody has been cited well over 50 times.
Date published: 2016-12-28
Rated 1 out of 5 by from No superhiftThis antibody did not work in EMSA to super shift the GATA-2 band generated with K562 nuclear extract.
Date published: 2016-11-11
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GATA-2 抗体 (CG2-96) is rated 4.6 out of 5 by 12.
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