Date published: 2025-9-8
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GAP-43 Antibody (B-5) - Western Blotting - Image 362030
GAP-43 抗体 (B-5): sc-17790. 大鼠大脑 (A), 小鼠脊髓 (B) 和大鼠小脑 (C) 组织提取物中 GAP-43 表达的 Western 印迹分析.
GAP-43 抗体 (B-5): sc-17790. 大鼠大脑 (A), 小鼠脊髓 (B) 和大鼠小脑 (C) 组织提取物中 GAP-43 表达的 Western 印迹分析.

GAP-43 抗体 (B-5): sc-17790

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说明书
  • GAP-43 抗体 B-5 是小鼠单克隆 IgG1 κ，GAP-43抗体, 在63篇文献中引用，规格为200 µg/ml
  • 免疫human来源GAP-43位于N-terminus 的1-100氨基酸
  • GAP-43 抗体 (B-5) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 axonal membrane protein GAP-43
  • 抗GAP-43抗体（B-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 另提供生物素偶联抗体，可用于WB、IHC(P)和ELISA实验
  • 关于如何获取GAP-43 (B-5): sc-17790的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 GAP-43 抗体 (B-5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与GAP-43 抗体 (B-5) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

GAP-43抗体（B-5）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的GAP-43蛋白。GAP-43抗体（B-5）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。GAP-43，又称生长相关蛋白43，在神经元发育和再生过程中发挥着至关重要的作用，主要表达于神经元生长锥。GAP-43的存在对于神经系统的突触连接和整体再生反应至关重要。GAP-43参与突触可塑性的调节，而突触可塑性对于学习和记忆至关重要。GAP-43与特定RNA结合蛋白（如神经元特异性Elav/Hu家族成员HuD）相互作用，可增强GAP-43 mRNA的稳定性，从而增加发育中神经元中的GAP-43水平。这种调节作用尤为重要，因为研究表明，杂合型GAP-43基因敲除小鼠的GAP-43水平降低50%，在记忆任务中表现出明显的障碍，这凸显了GAP-43在认知功能中的关键作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

GAP-43 抗体 (B-5) 参考文献:

  1. 油酸作为神经营养因子的作用在体内得到了支持，在脑部产后发育过程中，SREBP-1 激活后 GAP-43 的表达以及硬脂酰-CoA 去饱和酶的上调都支持了油酸作为神经营养因子的作用。  |  Velasco, A., et al. 2003. Brain Res. 977: 103-11. PMID: 12788519
  2. 空间记忆过程中 RNA 结合蛋白 HuD 的增加和 GAP-43 基因转录后的上调。  |  Pascale, A., et al. 2004. Proc Natl Acad Sci U S A. 101: 1217-22. PMID: 14745023
  3. 上橄榄核对听觉系统可塑性的贡献：内侧而非外侧橄榄核神经元是耳蜗切除术诱导的腹侧耳蜗核 GAP-43 表达的来源。  |  Kraus, KS. and Illing, RB. 2004. J Comp Neurol. 475: 374-90. PMID: 15221952
  4. 细胞骨架蛋白的表达水平预示着 S100B 转基因小鼠的病理衰老：对 MAP-2, drebrin 和 GAP-43 的免疫组化研究。  |  Shapiro, LA. and Whitaker-Azmitia, PM. 2004. Brain Res. 1019: 39-46. PMID: 15306236
  5. 生长锥中的 GAP-43 mRNA 与 HuD 和核糖体有关。  |  Smith, CL., et al. 2004. J Neurobiol. 61: 222-35. PMID: 15389607
  6. 神经调蛋白（GAP43）：神经元蛋白激酶 C 的底物，也存在于 0-2A 胶质细胞系中。少突胶质细胞二次培养物中神经调制蛋白的特征以及与神经元抗原的比较。  |  Deloulme, JC., et al. 1990. J Cell Biol. 111: 1559-69. PMID: 2170423
  7. 在形成偏好和厌恶味觉记忆的过程中，GAP-43或突触素发生差异性变化。  |  Grijalva, LE., et al. 2021. Behav Brain Res. 397: 112937. PMID: 32991926
  8. CSF GAP-43 的升高与阿尔茨海默氏症中 tau 的加速积累和扩散有关。  |  Franzmeier, N., et al. 2024. Nat Commun. 15: 202. PMID: 38172114
  9. 磷酸化 GAP-43（神经调制蛋白）在生长锥中的分布直接反映了生长锥的行为。  |  Dent, EW. and Meiri, KF. 1998. J Neurobiol. 35: 287-99. PMID: 9622012
  10. 神经元生长相关蛋白 GAP-43 与 rabaptin-5 相互作用并参与内吞。  |  Neve, RL., et al. 1998. J Neurosci. 18: 7757-67. PMID: 9742146
  11. GAP-43 与抗清洁剂膜的结合需要两个棕榈酰化半胱氨酸残基。  |  Arni, S., et al. 1998. J Biol Chem. 273: 28478-85. PMID: 9774477
  12. GAP-43 缺陷小鼠视交叉处视网膜神经节细胞轴突的随机路由：与中线交叉和同侧轴突缺乏正常转向有关。  |  Sretavan, DW. and Kruger, K. 1998. J Neurosci. 18: 10502-13. PMID: 9852588

订购信息

产品名称产品编号规格价格数量收藏夹

GAP-43 抗体 (B-5)

sc-17790
200 µg/ml
RMB2377.00

GAP-43 (B-5): m-IgG Fc BP-HRP 套装

sc-528316
200 µg Ab; 10 µg BP
RMB2662.00

GAP-43 (B-5): m-IgGκ BP-HRP 套装

sc-520660
200 µg Ab, 40 µg BP
RMB2662.00

GAP-43 (B-5): m-IgG1 BP-HRP 套装

sc-542863
200 µg Ab; 20 µg BP
RMB2662.00

GAP-43 抗体 (B-5) AC

sc-17790 AC
500 µg/ml, 25% agarose
RMB3129.00

GAP-43 抗体 (B-5) HRP

sc-17790 HRP
200 µg/ml
RMB2377.00

GAP-43 抗体 (B-5) FITC

sc-17790 FITC
200 µg/ml
RMB2482.00

GAP-43 抗体 (B-5) PE

sc-17790 PE
200 µg/ml
RMB2580.00

GAP-43 抗体 (B-5) Alexa Fluor® 488

sc-17790 AF488
200 µg/ml
RMB2685.00

GAP-43 抗体 (B-5) Alexa Fluor® 546

sc-17790 AF546
200 µg/ml
RMB2685.00

GAP-43 抗体 (B-5) Alexa Fluor® 594

sc-17790 AF594
200 µg/ml
RMB2685.00

GAP-43 抗体 (B-5) Alexa Fluor® 647

sc-17790 AF647
200 µg/ml
RMB2685.00

GAP-43 抗体 (B-5) Alexa Fluor® 680

sc-17790 AF680
200 µg/ml
RMB2685.00

GAP-43 抗体 (B-5) Alexa Fluor® 790

sc-17790 AF790
200 µg/ml
RMB2685.00

GAP-43 抗体 (B-5) B

sc-17790 B
200 µg/ml
RMB2407.00

Do you have recommended recombinant protein for ELISA use (with this Ab as capture)

Asked by: Yao Chen
Thank you for your question. GAP-43 Antibody (B-5): sc-17790 was raised against amino acids 1-100 mapping at the N-terminus of GAP-43 of human origin. A peptide containing this sequence should be detected by this antibody in ELISA applications. You can find the exact sequence on this product page under 'Protein Sequences'. Feel free to contact our technical service if you have further questions.
Answered by: Technical Support Europe
Date published: 2019-04-23

What is the best secondary to use with this GAP-43 antibody in Western Blot?

Asked by: sweetums
Thank you for your question. We recommend using our Mouse IgG kappa Binding Protein HRP, sc-516102. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Service 11
Date published: 2017-01-12

Can this mouse monoclonal antibody be used in IF or IHC on mouse tissue without background or non-specific staining issues?

Asked by: sweetums
Thank you for your question. Yes, it can be used successfully for this application. We recommend using a directly conjugated mouse monoclonal primary antibody. This mouse monoclonal antibody GAP-43 (B-5) is currently available conjugated to HRP, PE, FITC, AF488 and AF647.
Answered by: Technical Service 11
Date published: 2017-01-12
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Rated 5 out of 5 by from Great monoclonal antibodyGreat monoclonal antibody with strong bands and high specificity
Date published: 2017-09-05
Rated 5 out of 5 by from Superb reagent !!Very strong western blot data; also nice IHC staining
Date published: 2017-01-31
Rated 5 out of 5 by from Provides great signalThis antibody will work well in mouse, rat, or human samples. It provides strong WB bands and great signal in IHC. It has been published with nearly 20 times.
Date published: 2017-01-30
Rated 5 out of 5 by from 效果显著。申请试用了小样，做了小鼠嗅上皮组织的免疫组化，1:100使用。荧光二抗1:200使用。显微镜观察，GAP43在嗅上皮位置有明显阳性表达。
Date published: 2017-01-05
Rated 5 out of 5 by from Good resultsWe good nice bands using rat cortex protein extraction.
Date published: 2016-12-12
Rated 4 out of 5 by from Potent and very specific antibodyThis monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was medium detection of the ~43 kDa target protein in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested.
Date published: 2016-11-10
Rated 5 out of 5 by from 1:1000 dilution used for western blot with1:1000 dilution used for western blot with MN9D/CRFR2 cell lysates. -SCBT Publication Review
Date published: 2015-07-19
Rated 5 out of 5 by from Clean bands in western blot analysis of GAPClean bands in western blot analysis of GAP-43 expression in mouse and rat brain tissue extract. -SCBT QC
Date published: 2015-07-09
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GAP-43 抗体 (B-5) is rated 4.9 out of 5 by 11.
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