Date published: 2025-9-6
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Ganglioside GD2 抗体 (14G2a): sc-53831

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说明书
  • Ganglioside GD2 抗体 14G2a 是小鼠单克隆 IgG2a κ，Ganglioside GD2抗体, 在23篇文献中引用，规格为200 µg/ml
  • 针对human来源的神经外胚层肿瘤细胞
  • Ganglioside GD2 抗体 (14G2a) 推荐用于IF, IHC(P) 和 FCM，检测human 来源的 Ganglioside GD2
  • 抗Ganglioside GD2抗体（14G2a）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Ganglioside GD2 (14G2a): sc-53831的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是Ganglioside GD2 Antibody (14G2a) 适用于 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与Ganglioside GD2 Antibody (14G2a) 打包提供（请参阅下面的订购信息）。

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研究信息

関連項目

额外Ganglioside抗体，包括Ganglioside GD2, Ganglioside GD3和GM2/GD2 Synthase

神经节苷脂 GD2 抗体（14G2a）是一种小鼠单克隆 IgG2a kappa 轻链抗体，可通过免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和流式细胞术（FCM）检测人源神经节苷脂 GD2 蛋白。抗神经节苷脂 GD2 抗体 (14G2a) 有非共轭型和多种共轭型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 共轭物。神经节苷脂 GD2 是一种含硅醛酸的糖蛋白脂，在细胞信号传导和识别中起着至关重要的作用，因此神经节苷脂 GD2 对各种细胞过程至关重要。神经节苷脂 GD2 存在于细胞表面有利于细胞与其环境之间的相互作用，影响细胞粘附和交流。神经节苷脂 GD2 在神经母细胞瘤细胞和其他来源于神经外胚层的肿瘤（如恶性黑色素瘤和某些胃肠道癌症）中高表达，这凸显了神经节苷脂 GD2 作为免疫疗法靶点的潜力。GM2/GD2合成酶调节神经节苷脂GD2的产生，神经节苷脂GD2通过病灶粘附激酶去磷酸化参与细胞凋亡调节，这些都凸显了神经节苷脂GD2在癌症生物学中的重要性。神经节苷脂 GD2 在间质基质细胞上的独特表达模式表明它有可能被用作一种独特的表面标记物，强调了它在诊断和治疗应用中的相关性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Ganglioside GD2 抗体 (14G2a) 参考文献:

  1. FCGR2A多态性与使用抗GD2抗体和粒细胞巨噬细胞集落刺激因子对神经母细胞瘤进行免疫治疗后的临床结果有关。  |  Cheung, NK., et al. 2006. J Clin Oncol. 24: 2885-90. PMID: 16682723
  2. 一名抗体治疗后未复发的神经母细胞瘤患者的抗生物素免疫反应的分子特征：一种针对神经外胚层肿瘤的可能疫苗？  |  Uttenreuther-Fischer, MM., et al. 2006. J Immunol. 176: 7775-86. PMID: 16751426
  3. 多发性硬化症患者体内的抗 GD2 类 IgM 自身反应。  |  Marconi, S., et al. 2006. Mult Scler. 12: 302-8. PMID: 16764343
  4. 对晚期视网膜母细胞瘤进行积极的骨髓评估，包括使用 GD2 进行免疫细胞学检查。  |  Chantada, GL., et al. 2006. J Pediatr Hematol Oncol. 28: 369-73. PMID: 16794505
  5. 以神经节苷脂 GD3 合成酶基因为肺癌治疗靶点的小干扰 RNA 基础研究。  |  Ko, K., et al. 2006. Oncogene. 25: 6924-35. PMID: 16862187
  6. 受肿瘤相关碳水化合物抗原调节的生物信号：癌症治疗的新靶点。  |  Furukawa, K., et al. 2006. Ann N Y Acad Sci. 1086: 185-98. PMID: 17185516
  7. 脱落神经节苷脂为 3 型糖苷键提供了独立于去污剂的证据。  |  Thorne, RF., et al. 2007. Biochem Biophys Res Commun. 356: 306-11. PMID: 17350595
  8. 针对神经母细胞瘤的二异抗原苷免疫疗法。  |  Modak, S. and Cheung, NK. 2007. Cancer Invest. 25: 67-77. PMID: 17364560
  9. 从受限噬菌体显示的肽库中筛选出 GD2 神经节苷脂的新型肽模拟物。  |  Horwacik, I., et al. 2007. Int J Mol Med. 19: 829-39. PMID: 17390090
  10. 基于 RNA 测序的神经节苷脂 GD2 阳性癌症表型鉴定  |  Sorokin, M., et al. 2020. Biomedicines. 8: PMID: 32486168
  11. 神经节苷脂 GD2 通过与整合素合作增强黑色素瘤细胞的恶性表型  |  Yesmin, F., et al. 2021. Int J Mol Sci. 23: PMID: 35008849
  12. 神经节苷脂 GD2 表达与早期三阴性乳腺癌的不良预后有关  |  Higashi, C., et al. 2023. Anticancer Res. 43: 4045-4053. PMID: 37648305

订购信息

产品名称产品编号规格价格数量收藏夹

Ganglioside GD2 抗体 (14G2a)

sc-53831
200 µg/ml
RMB2377.00

Ganglioside GD2 (14G2a): m-IgG Fc BP-HRP 套装

sc-528579
200 µg Ab; 10 µg BP
RMB2662.00

Ganglioside GD2 (14G2a): m-IgGκ BP-HRP 套装

sc-520990
200 µg Ab, 40 µg BP
RMB2662.00

Ganglioside GD2 (14G2a): m-IgG2a BP-HRP 套装

sc-547124
200 µg Ab; 10 µg BP
RMB2662.00

Ganglioside GD2 抗体 (14G2a) AC

sc-53831 AC
500 µg/ml, 25% agarose
RMB3129.00

Ganglioside GD2 抗体 (14G2a) HRP

sc-53831 HRP
200 µg/ml
RMB2377.00

Ganglioside GD2 抗体 (14G2a) FITC

sc-53831 FITC
200 µg/ml
RMB2482.00

Ganglioside GD2 抗体 (14G2a) PE

sc-53831 PE
200 µg/ml
RMB2580.00

Ganglioside GD2 抗体 (14G2a) Alexa Fluor® 488

sc-53831 AF488
200 µg/ml
RMB2685.00

Ganglioside GD2 抗体 (14G2a) Alexa Fluor® 546

sc-53831 AF546
200 µg/ml
RMB2685.00

Ganglioside GD2 抗体 (14G2a) Alexa Fluor® 594

sc-53831 AF594
200 µg/ml
RMB2685.00

Ganglioside GD2 抗体 (14G2a) Alexa Fluor® 647

sc-53831 AF647
200 µg/ml
RMB2685.00

Ganglioside GD2 抗体 (14G2a) Alexa Fluor® 680

sc-53831 AF680
200 µg/ml
RMB2685.00

Ganglioside GD2 抗体 (14G2a) Alexa Fluor® 790

sc-53831 AF790
200 µg/ml
RMB2685.00

Hi, We are research team from Mayo Clinic, and we are interested in the Ganglioside GD2 antibody (14G2a) I am wondering if by any chance we can obtain free sample of IF: Alexa Fluor 647 conjugated, and free sample for HRP conjugated. Thank you .

Asked by: Zt0508
Thank you for your question. For information about our sample program please contact Technical Service. Please call 800-457-3801 or email [email protected].
Answered by: BlakeJ
Date published: 2025-09-01

What is the recommended dilution to use for this ab?

Asked by: amionescu
Dilution depends on the assay. Contact Technical Service or referral to our Protocols for more detailed information. https://www.scbt.com/resources/protocols
Answered by: Tech Sales
Date published: 2021-07-09

Hi, Just wondering whether you have the sequence for the variable region of GD-2 antibody or can you provide the hybridoma for sequencing. Thank you. Jue

Asked by: Jack cells
Thank you for your question. This antibody was raised against neuroectodermal tumor cells of human origin. The exact epitope has not been epitope mapped. We are not able to provide the hybridoma for sequencing.
Answered by: Tech Service
Date published: 2019-09-04

Dose Ganglioside GD2 (14G2a) has certificated of anaylsis for QC department for validation use?

Asked by: JR Chen
Thank you for your question. We do not provide a certificate of analysis for any of our research antibodies. Please contact our Technical Service Department for any specific questions about how the antibody has been tested. Please call our Asian Support office toll-free at 800-820-8626, email [email protected], or contact us by live chat.
Answered by: Technical Support
Date published: 2018-08-27

From the description of the product, I understand that the 14(G2a) antibody is provided as the kappa light chain of the antibody. How can the kappa light chain recognise the GD2 sugar without the heavy chain? Many thanks

Asked by: DMax
Thank you for the question. The Ganglioside GD2 (14G2a) is a full mouse IgG2a antibody. We characterized the type of light chain because instead of conventional secondary antibodies against full-IgG we instead use a light-chain-specific binding protein which tends to produce cleaner results compared to conventional secondary antibodies. In this case we suggest using m-IgGκappa-specific BP-HRP: sc-516102 for the detection of Ganglioside GD2 (14G2a) sc-53831.
Answered by: Technical Support Europe
Date published: 2018-01-10

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: Cweed
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: TechService7
Date published: 2016-12-22

How can this mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: Cweed
When attempting a double or triple stain with mouse monoclonal antibodies, we recommend using a directly conjugated primary antibody. This product, sc-53831, is available conjugated to FITC, AF488, and AF647.
Answered by: TechService7
Date published: 2016-12-22
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Rated 1 out of 5 by from Troppo debole in IHC(P)Testato per IHC(P) con più antigen retrieval, il segnale resta molto debole (quasi invisibile) nonostante incubazione over night e diluizione 1:30.
Date published: 2025-06-11
Rated 2 out of 5 by from Unzufriedenes Ergebnis in der IHCAntikörper vor etwa 3 Wochen bekommen. Schnelle Lieferung. Antikörper hat nicht die gewünschte Färbung an Paraffinschnitten geziegt
Date published: 2018-04-16
Rated 5 out of 5 by from nicely stains cultured neuroblastoma cellsImmunofluorescence analysis of cultured human neuroblastoma cells fixed with paraformaldehyde. Samples were incubated with sc-53831 GD2 (14G2a) antibody (1:200) in 1% BSA + 1% serum for 1 h. An anti-mouse AlexaFluor®-488 was used as secondary antibody (1:400).
Date published: 2018-02-13
Rated 5 out of 5 by from specificity and cleanDistinct band, clean background observed by WB using Ganglioside GD2 antibody.
Date published: 2017-05-07
Rated 5 out of 5 by from Published antibodyGanglioside GD2 Antibody (14G2a) has been cited in several publications
Date published: 2017-01-12
Rated 5 out of 5 by from This antibody was used in a publication forThis antibody was used in a publication for FCM on human cell culture sample - SCBT Publication Review
Date published: 2015-03-23
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Ganglioside GD2抗体 (14G2a) is rated 3.8 out of 5 by 6.
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