Date published: 2025-9-6
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γ Tubulin Antibody (C-11) - Western Blotting - Image 5459
γ微管蛋白 (C-11): sc-17787. 对 HeLa (A), A-431 (B), K-562 (C), NIH/3T3 (D) 和 KNRK (E) 全细胞裂解液中 γ 管蛋白表达的 Western 印迹分析.
γ微管蛋白 (C-11): sc-17787. 对 HeLa (A), A-431 (B), K-562 (C), NIH/3T3 (D) 和 KNRK (E) 全细胞裂解液中 γ 管蛋白表达的 Western 印迹分析.

γ Tubulin 抗体 (C-11): sc-17787

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寫評論提問

说明书
  • gamma Tubulin 抗体 C-11 是小鼠单克隆 IgG2a κ，γ Tubulin抗体, 在97篇文献中引用，规格为200 µg/ml
  • 免疫human物种的γTubulin的氨基酸269-451
  • gamma Tubulin 抗体 (C-11) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 γ Tubulin
  • 抗gamma Tubulin抗体（C-11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取γ Tubulin (C-11): sc-17787的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 γ Tubulin 抗体 (C-11) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与γ Tubulin 抗体 (C-11) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 Tubulin 抗体 包括 α 1 Tubulin, α 3 Tubulin, α 4 Tubulin, α Tubulin, β Tubulin, β 2 Tubulin, β 3 Tubulin, δ Tubulin, ε Tubulin和γ Tubulin

γ Tubulin 抗体（C-11）是一种小鼠单克隆 IgG2a kappa 轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的 γ Tubulin 蛋白。抗 γ Tubulin 抗体（ C-11）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。伽马微管蛋白在微管组织中起着至关重要的作用， 微管细胞骨架的形成，而微管细胞骨架对于细胞分裂、细胞内运输和维持细胞形状等许多细胞过程至关重要。伽马微管蛋白是伽马微管复合体的重要组成部分，该复合体在中心体上充当微管组装的成核位点，从而促进有丝分裂期间微管的正确组织。该功能可确保细胞分裂期间染色体准确分离和稳定，这对于维持基因组完整性至关重要。伽马微管蛋白与其它蛋白（如与中心体相关的δ微管蛋白和ε微管蛋白）相互作用，进一步凸显了伽马微管蛋白在微管网络和细胞结构中的不可或缺的作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

γ Tubulin 抗体 (C-11) 参考文献:

  1. γ-微管蛋白复合物形成微管。  |  Kollman, JM., et al. 2011. Nat Rev Mol Cell Biol. 12: 709-21. PMID: 21993292
  2. γ-微管蛋白复合物介导的微管成核调控。  |  Sulimenko, V., et al. 2017. Protoplasma. 254: 1187-1199. PMID: 28074286
  3. γ-微管蛋白作为信号转导分子和具有治疗潜力的网络。  |  Alvarado-Kristensson, M. 2018. Signal Transduct Target Ther. 3: 24. PMID: 30221013
  4. γ-微管蛋白复合物及其他微管成核。  |  Tovey, CA. and Conduit, PT. 2018. Essays Biochem. 62: 765-780. PMID: 30315097
  5. γ-微管蛋白与γ-微管蛋白相互作用是形成网状结构的基础。  |  Rosselló, CA., et al. 2018. Int J Mol Sci. 19: PMID: 30347727
  6. γ-微管蛋白复合物和纤维状阵列：两种具有多种细胞功能的保守的高分子形式。  |  Chumová, J., et al. 2021. Cells. 10: PMID: 33915825
  7. CAMSAP2 通过相分离组织了一个不依赖于γ-微管蛋白的微管成核中心。  |  Imasaki, T., et al. 2022. Elife. 11: PMID: 35762204
  8. 有丝分裂中心体上γ-微管蛋白复合物招募和微管成核的多重模式  |  Zhu, Z., et al. 2023. J Cell Biol. 222: PMID: 37698931
  9. 人类γ-微管蛋白环复合物在微管成核过程中转变为封闭构象。  |  Brito, C., et al. 2024. Science. 383: 870-876. PMID: 38305685
  10. γ-微管蛋白环复合物覆盖微管的结构。  |  Aher, A., et al. 2024. Nat Struct Mol Biol. 31: 1124-1133. PMID: 38609661

订购信息

产品名称产品编号规格价格数量收藏夹

γ Tubulin 抗体 (C-11)

sc-17787
200 µg/ml
RMB2377.00

γ Tubulin (C-11): m-IgG Fc BP-HRP 套装

sc-528314
200 µg Ab; 10 µg BP
RMB2662.00

γ Tubulin (C-11): m-IgGκ BP-HRP 套装

sc-520658
200 µg Ab, 40 µg BP
RMB2662.00

γ Tubulin (C-11): m-IgG2a BP-HRP 套装

sc-547070
200 µg Ab; 10 µg BP
RMB2662.00

γ Tubulin 抗体 (C-11) AC

sc-17787 AC
500 µg/ml, 25% agarose
RMB3129.00

γ Tubulin 抗体 (C-11) HRP

sc-17787 HRP
200 µg/ml
RMB2377.00

γ Tubulin 抗体 (C-11) FITC

sc-17787 FITC
200 µg/ml
RMB2482.00

γ Tubulin 抗体 (C-11) PE

sc-17787 PE
200 µg/ml
RMB2580.00

γ Tubulin 抗体 (C-11) Alexa Fluor® 488

sc-17787 AF488
200 µg/ml
RMB2685.00

γ Tubulin 抗体 (C-11) Alexa Fluor® 546

sc-17787 AF546
200 µg/ml
RMB2685.00

γ Tubulin 抗体 (C-11) Alexa Fluor® 594

sc-17787 AF594
200 µg/ml
RMB2685.00

γ Tubulin 抗体 (C-11) Alexa Fluor® 647

sc-17787 AF647
200 µg/ml
RMB2685.00

γ Tubulin 抗体 (C-11) Alexa Fluor® 680

sc-17787 AF680
200 µg/ml
RMB2685.00

γ Tubulin 抗体 (C-11) Alexa Fluor® 790

sc-17787 AF790
200 µg/ml
RMB2685.00

How this antibody immobilizes primary cells？

Asked by: chalice
感谢您的提问。我不理解您的提问，请联系我们的技术支持部门。亚洲技术支持电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”。谢谢。 Thank you for your question. I do not understand your question, please contact our technical support department. Asia Technical Support at (+86) 021-60936350, [email protected], or select "Chinese Support" in the Live Chat section of the website. Thank you.
Answered by: Jiajun Z
Date published: 2024-01-25

Can this mouse monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: sweetums
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, our gamma Tubulin sc-17787 is available in a variety of direct conjugations, such as HRP, PE, FITC, AlexaFluor 488 and AlexaFluor 647.
Answered by: Technical Support 12
Date published: 2017-01-12

Is there any data showing this antibody is specific to gamma Tubulin?

Asked by: sweetums
Thank you for your question. We have immunofluorescence data with methanol fixed HeLa cells treated with siRNA to show knockdown of signal, confirming specificity to gamma Tubulin.
Answered by: Technical Support 12
Date published: 2017-01-12
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Rated 4 out of 5 by from IF stainingI used this antibody for IF on 4% PFA fixed cells, diluting it 1:500. Specific for centrosomes, with some nuclear and cytoplasmic background
Date published: 2022-04-05
Rated 4 out of 5 by from GoodI used this wnatibody for western blot. I diluted it at 1:1000 and it works well.
Date published: 2021-04-16
Rated 5 out of 5 by from Specific antibody for immunostainingWorked well for visualization of centrosomes (Confocal microscopy): Specific and very low background
Date published: 2021-03-23
Rated 5 out of 5 by from 蛍光免疫染色4%PFA固定したラット組織の凍結切片と、賦活化処理したパラフィン切片に対して蛍光免疫染色を行いました。どちらの条件でも、特異的なシグナルが得られました。また、希釈倍率も500～1000倍で使用でき、満足しています。
Date published: 2018-11-26
Rated 5 out of 5 by from funciona bienFunciona bien en inmunofluorescencia con dilucion 1:100
Date published: 2018-04-16
Rated 5 out of 5 by from Very well resultsThis antibody generates a clear and strong signal at the predicted position. Very good.
Date published: 2018-01-25
Rated 5 out of 5 by from Good antibodyNice antibody working in IF, WB for mouse cell line
Date published: 2017-10-12
Rated 5 out of 5 by from Tested for IF: very good stainingTested at 2 ug/ml on PFA/MeOH fix/perm primary cells. very good results
Date published: 2017-10-05
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γ Tubulin抗体 (C-11) is rated 4.6 out of 5 by 18.
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