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G<sub>α s/olf</sub> Antibody (A-5) - Western Blotting - Image 14618 — G_{α s/olf} (A-5): sc-55545. SK-N-MC (A) 和 T98G (B) 全细胞裂解液中 G_{α s} 和 G_{α olf} 表达的 Western 印迹分析.

G_{α s/olf} (A-5): sc-55545. SK-N-MC (A) 和 T98G (B) 全细胞裂解液中 G_{α s} 和 G_{α olf} 表达的 Western 印迹分析.

G_{α s/olf} 抗体 (A-5): sc-55545

Name: G&alpha; s/olf抗体 (A-5)
Brand: SCBIO
SKU: sc-55545
Price: 2377.0 CNY
Availability: InStock

5.0(9)

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说明书

G_{α s/olf}抗体(A-5)是小鼠单克隆IgG₁ κ, 在27篇文献中引用，规格为200 µg/ml
针对氨基酸82-381，在Gα s/olf的human起源的C-terminus处进行映射
抗-G alpha s/olf 抗体 (A-5) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 G_{α s1-4}and G_{α olf}
抗G alpha s/olf抗体（A-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
还可偶联Alexa Fluor^® 488, Alexa Fluor^® 546, Alexa Fluor^® 594 和 Alexa Fluor^® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
还可偶联Alexa Fluor^® 680 和 Alexa Fluor^® 790，用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR^®/Odyssey^®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
关于如何获取G_{α s/olf} (A-5): sc-55545的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
m-IgG Fc BP-HRP, m-IgG₁ BP-HRP 和 m-IgGκ BP-HRP 是 G_{α s/olf} 抗体 (A-5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与G_{α s/olf} 抗体 (A-5) 组合成套装提供。 (请参阅下面的订购信息)。

查看产品引用文献(25)

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G_{α s/olf} 抗体 (A-5) 参考文献:

不同的 beta 亚基决定了 G 蛋白与跨膜受体的相互作用。 | Kleuss, C., et al. 1992. Nature. 358: 424-6. PMID: 1322501
G 蛋白 gamma 亚基的选择性组织分布，包括通过 cDNA 克隆发现的一种新形式的 gamma 亚基。 | Cali, JJ., et al. 1992. J Biol Chem. 267: 24023-7. PMID: 1385432
在 Sf9 细胞中合成的 G 蛋白 beta γ 亚基。G 蛋白 beta γ 亚基在 Sf9 细胞中合成。 | Iñiguez-Lluhi, JA., et al. 1992. J Biol Chem. 267: 23409-17. PMID: 1429682
异三聚体 GTP 结合蛋白 beta 亚基的多样性：新型 beta 亚基 cDNA 的特征。 | von Weizsäcker, E., et al. 1992. Biochem Biophys Res Commun. 183: 350-6. PMID: 1543505
Gustducin 是一种味觉细胞特异性 G 蛋白，与转导蛋白关系密切。 | McLaughlin, SK., et al. 1992. Nature. 357: 563-9. PMID: 1608467
研究G蛋白亚基Galphaolf基因（GNAL）在注意力缺陷/多动症中的作用。 | Laurin, N., et al. 2008. J Psychiatr Res. 42: 117-24. PMID: 17166517
信号转导中 G 蛋白的多样性。 | Simon, MI., et al. 1991. Science. 252: 802-8. PMID: 1902986
Golf：参与气味信号转导的嗅觉神经元特异性-G 蛋白。 | Jones, DT. and Reed, RR. 1989. Science. 244: 790-5. PMID: 2499043
G蛋白亚基磷酸化作为哺乳动物、酵母菌和植物中异源三聚体G蛋白信号转导的调控机制。 | Chakravorty, D. and Assmann, SM. 2018. Biochem J. 475: 3331-3357. PMID: 30413679
G 蛋白亚基α i2 在血管生成中的关键作用 | Bai, CW., et al. 2024. Theranostics. 14: 2190-2209. PMID: 38505600
信号转导的选择性由异源三聚体 G 蛋白的 gamma 亚基决定。 | Kleuss, C., et al. 1993. Science. 259: 832-4. PMID: 8094261
与 G beta gamma, 受体和效应器相互作用的 G alpha 亚基的结构元素。 | Conklin, BR. and Bourne, HR. 1993. Cell. 73: 631-41. PMID: 8388779

订购信息

产品名称	产品编号	规格	价格	数量	收藏夹
G_{α s/olf} 抗体 (A-5)	sc-55545	200 µg/ml	RMB2377.00
G_{α s/olf} (A-5): m-IgG Fc BP-HRP 套装	sc-528598	200 µg Ab; 10 µg BP	RMB2662.00
G_{α s/olf} (A-5): m-IgGκ BP-HRP 套装	sc-521014	200 µg Ab, 40 µg BP	RMB2662.00
G_{α s/olf} (A-5): m-IgG₁ BP-HRP 套装	sc-543018	200 µg Ab; 20 µg BP	RMB2662.00
Gα s/olf 抗体 (A-5) AC	sc-55545 AC	500 µg/ml, 25% agarose	RMB3129.00
Gα s/olf 抗体 (A-5) HRP	sc-55545 HRP	200 µg/ml	RMB2377.00
Gα s/olf 抗体 (A-5) FITC	sc-55545 FITC	200 µg/ml	RMB2482.00
Gα s/olf 抗体 (A-5) PE	sc-55545 PE	200 µg/ml	RMB2580.00
Gα s/olf 抗体 (A-5) Alexa Fluor^® 488	sc-55545 AF488	200 µg/ml	RMB2685.00
G_{α s/olf} 抗体 (A-5) Alexa Fluor^® 546	sc-55545 AF546	200 µg/ml	RMB2685.00
Gα s/olf 抗体 (A-5) Alexa Fluor^® 594	sc-55545 AF594	200 µg/ml	RMB2685.00
Gα s/olf 抗体 (A-5) Alexa Fluor^® 647	sc-55545 AF647	200 µg/ml	RMB2685.00
Gα s/olf 抗体 (A-5) Alexa Fluor^® 680	sc-55545 AF680	200 µg/ml	RMB2685.00
Gα s/olf 抗体 (A-5) Alexa Fluor^® 790	sc-55545 AF790	200 µg/ml	RMB2685.00

What is meant by raised against Gas/olf? Is it that the peptide sequence used is identical between two different Ga isoforms and thus the antibody targets the same epitope?

Asked by: Brian29

For more information Please Contact Technical Service.

Answered by: Tech Sales

Date published: 2021-10-26

I received this antibody (sc-55545) yesterday, and it just says that it has to be kept in 4°C. Do we need to add something inside the tube (like glycerol as for some other antibodies) to better conserve the antibody or is it fine like it is at 4°C ?

Asked by: scnath

Thank you for your question. There is no need to add anything to the antibody, please just store it at 4˚C and use as directed. It will be fine if stored properly at 4˚C.

Answered by: Tech Service

Date published: 2019-10-03

I had a aliquot of this antibody (sc55545) lot #G1116 and I want to know whats is it expiration date.

Asked by: VictorX

Thank you for your question. We do not characterize expiration dates for our research antibodies. They are warrantied for one year from the date of purchase. However, when stored properly at 4C they can continue to be viable for much longer than the one year warranty period.

Answered by: Technical Support

Date published: 2019-05-01

Can Gα s/olf (A-5): sc-55545 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Randy McDonald

Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Gα s/olf (A-5): sc-55545 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.

Answered by: Technical Support

Date published: 2017-02-24

Rated 5 out of 5 by samtang from G-α s/olf 抗体 (A-5)抗体真不错，效果很好！我一个月之前买的这个抗体，当时是根据价格选的比较经济实惠，没想到做的实验效果非常好，老师非常满意，真的不错！

Date published: 2017-11-03

Rated 5 out of 5 by devendra from Perfect for olfactory epitheliumAntibody works perfect with frozen section of mouse olfactory epithelium. Tissue was fixed with PFA. Antibody gives very specific signals in ciliary part of olfactory epithelium.

Date published: 2017-10-05

Rated 5 out of 5 by Doctor Chijiangyang from 抗体很好用的用的小样申请装抗体，效果还不错，WB显色没有那么亮，但完全符合要求，推荐使用，做免疫组化的话，效果还不确定

Date published: 2017-08-29

Rated 5 out of 5 by ge_lin from Great for WBWith this antibody using 1:1000 for Western Blot analysis, antibody is very stable and shows no non-specific bands or background for WB.

Date published: 2017-05-07

Rated 5 out of 5 by liujia from western blotUsed for western blot with D-5 with murine kidney samples, worked well in western blotting.

Date published: 2017-02-25

Rated 5 out of 5 by MS from Bands observed at the expected molecularBands observed at the expected molecular weight for G s/olf proteins by Western Blot using SK-N-MC and T98G whole cell lysates. -SCBT QC

Date published: 2015-04-23

Rated 5 out of 5 by JM from NuclearNuclear, cytoplasmic and membrane staining of urothelial cells observed by immunoperoxidase staining of formalin fixed, paraffin embedded human urinary bladder tissue. -SCBT QC

Date published: 2015-04-22

Rated 5 out of 5 by FL from Publishable data obtained by immunohistochemistryPublishable data obtained by immunohistochemistry using african lungfish nasal sac and brain. -SCBT Publication Review

Date published: 2015-01-20

G α s/olf 抗体 (A-5) Reviews - page 2

G α s/olf 抗体 (A-5) is rated 5.0 out of 5 by 9.

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种属	基因名称	基因ID	染色体位置	亚型（mRNA）登录号	蛋白质登录号	OMIM™ 号码
Human	GNAL	Entrez Gene ID: 2774	18p11.21	XM_006722324, NM_001142339, NM_001261443, NM_001261444, NM_182978, NM_002071	UniProt ID: P38405	139312
Mouse	Gnal	Entrez Gene ID: 14680	18 E1	NM_010307, NM_177137	UniProt ID: Q8CGK7	N/A

G_{α s/olf} 抗体 (A-5): sc-55545

G_{α s/olf} 抗体 (A-5) 参考文献:

G_{α s/olf} 抗体 (A-5) 规格

替代产品名称: G-protein subunit GNAS locus / GNAL Olfactory

产品克隆名称: A-5

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgG₁ kappa light chain

氨基酸: 82-381

抗原表位靶标: Gα s/olf

抗原表位种属: human

产品反应性/检测: G_{α s1-4}and G_{α olf}

种属反应性/检测: mouse, rat, human

其它哺乳动物反应性: N/A

其它非哺乳动物反应性: N/A

应用: WB, IP, IF, IHC(P), ELISA

瓶的信息: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文: 27篇各种科学出版物的引文

G_{α s/olf} 抗体 (A-5)可选的偶联物/形式: AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

優選的 G_{α s} 抗體是 G_{α s} 抗體 (12): sc-135914.