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G<sub>α i-3</sub> Antibody (H-7) - Western Blotting - Image 353732 — Gα i-3 抗体 (H-7): sc-365422. 小鼠脑 (A), 大鼠脑 (B) 和大鼠肾 (C) 组织提取物以及 Hep G2 全细胞裂解液 (D) 中 Gα i-3 表达的 Western 印迹分析.

Gα i-3 抗体 (H-7): sc-365422. 小鼠脑 (A), 大鼠脑 (B) 和大鼠肾 (C) 组织提取物以及 Hep G2 全细胞裂解液 (D) 中 Gα i-3 表达的 Western 印迹分析.

G_{α i-3} 抗体 (H-7): sc-365422

Name: G&alpha; i-3抗体 (H-7)
Brand: SCBIO
SKU: sc-365422
Price: 2377.0 CNY
Availability: InStock

5.0(8)

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说明书

G_{α i-3}抗体(H-7)是小鼠单克隆IgG₃ κ, 在14篇文献中引用，规格为200 µg/ml
特异性抗原位于rat物种的G_{α i-3}的C-terminus的氨基酸339-354之间
推荐用于 mouse, rat， human 和来源的G_{α i-1} and G_{α i-3} WB, IP, IF 和 ELISA检测; 也和以下物种反应，包括：和 equine, canine, bovine, porcine and avian
另提供琼脂糖偶联抗体，规格为琼脂糖装，可用于IP实验；HRP 偶联抗体用于WB, IHC(P)和ELISA实验；以及藻红蛋白、FITC、Alexa Fluor^® 488 或Alexa Fluor^® 647 偶联抗体，规格为用于IF、IHC(P)和FCM实验
关于如何获取G_{α i-3} (H-7): sc-365422的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
m-IgGκ BP-HRP是G_{α i-3} Antibody (H-7) 适用于 WB 应用。的首选辅助检测试剂。该试剂现可与G_{α i-3} Antibody (H-7) 搭配使用（请参阅下面的订购信息）。有关其它 m-IgGκ BP 结合物，请参阅我们完整的小鼠 IgG 结合蛋白列表。

查看产品引用文献(14)

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G_{α i-3} 抗体 (H-7) 参考文献:

G 蛋白 gamma 亚基的选择性组织分布，包括通过 cDNA 克隆发现的一种新形式的 gamma 亚基。 | Cali, JJ., et al. 1992. J Biol Chem. 267: 24023-7. PMID: 1385432
异三聚体 GTP 结合蛋白 beta 亚基的多样性：新型 beta 亚基 cDNA 的特征。 | von Weizsäcker, E., et al. 1992. Biochem Biophys Res Commun. 183: 350-6. PMID: 1543505
Gustducin 是一种味觉细胞特异性 G 蛋白，与转导蛋白关系密切。 | McLaughlin, SK., et al. 1992. Nature. 357: 563-9. PMID: 1608467
氟烷对 galphai-3 及其与 M2 肌激肽受体耦合的影响 | Jin, F., et al. 2005. Anesthesiology. 103: 1015-25. PMID: 16249676
信号转导中 G 蛋白的多样性。 | Simon, MI., et al. 1991. Science. 252: 802-8. PMID: 1902986
三种刺激性 GTP 结合蛋白的生化特征。大, 小 Gs 和嗅觉特异性 G 蛋白 Golf。 | Jones, DT., et al. 1990. J Biol Chem. 265: 2671-6. PMID: 2105931
GNAI1和GNAI3通过阻断IL6信号传导和下调GNAI2的表达，减少小鼠结肠炎相关的肿瘤发生。 | Li, ZW., et al. 2019. Gastroenterology. 156: 2297-2312. PMID: 30836096
5-HT1A受体偶联的Gαi3 G蛋白的钟形激动剂激活：受体密度依赖的受体信号传导开关。 | Newman-Tancredi, A., et al. 2019. Cell Signal. 63: 109383. PMID: 31376526
超越G蛋白α亚基：研究Gαi3异三聚体其他成分的功能影响。 | Rysiewicz, B., et al. 2023. Cell Commun Signal. 21: 279. PMID: 37817242
Gαi蛋白中的多碱基区：相关与否？Gαi3研究中的见解。 | Rysiewicz, B., et al. 2024. Cell Signal. 118: 111138. PMID: 38467243
与 G beta gamma, 受体和效应器相互作用的 G alpha 亚基的结构元素。 | Conklin, BR. and Bourne, HR. 1993. Cell. 73: 631-41. PMID: 8388779
GAIP是一种与高尔基体衍生的囊泡和蛋白质运输相关的Galphai-3结合蛋白。 | Wylie, F., et al. 1999. Am J Physiol. 276: C497-506. PMID: 9950778

订购信息

产品名称	产品编号	规格	价格	数量	收藏夹
G_{α i-3} 抗体 (H-7)	sc-365422	200 µg/ml	RMB2377.00
G_{α i-3} (H-7): m-IgGκ BP-HRP 套装	sc-522241	200 µg Ab, 40 µg BP	RMB2662.00
G_{α i-3} 抗体 (H-7) AC	sc-365422 AC	500 µg/ml, 25% agarose	RMB3129.00
G_{α i-3} 抗体 (H-7) Alexa Fluor^® 488	sc-365422 AF488	200 µg/ml	RMB2685.00
G_{α i-3} 抗体 (H-7) Alexa Fluor^® 647	sc-365422 AF647	200 µg/ml	RMB2685.00
G_{α i-3} 抗体 (H-7) FITC	sc-365422 FITC	200 µg/ml	RMB2482.00
G_{α i-3} 抗体 (H-7) HRP	sc-365422 HRP	200 µg/ml	RMB2377.00
Gα i-3 (H-7) 中和勝肽	sc-365422 P	100 µg/0.5 ml	RMB511.00
G_{α i-3} 抗体 (H-7) PE	sc-365422 PE	200 µg/ml	RMB2580.00

Does this antibody work well in Raw 264.7 and Jurkat cell lines?

Asked by: little star123

Thank you for your question. We do not currently have any data with this antibody in either of these cell lines.

Answered by: Technical Support

Date published: 2018-11-30

How likely is it that this antibody will show a cross reaction to Gnai2 since they are similar in structure? Was this tested? How similar is the immunogen the antibody was raised against to Gnai2? Thanks in advance!

Asked by: Gwen

Thank you for your question. This mouse monoclonal antibody, Gα i-3 (H-7): sc-365422, is specific for an epitope mapping between amino acids 339-354 at the C-terminus of Gα i-3 of rat origin. This region does share significant identity (80%) with the Gα i-2 protein, so there is potential of cross-reactivity. Unfortunately, this cross-reactivity has not yet been tested. For a limited time, we are offering a free sample of this antibody. Please contact our Technical Service Department if you would like to request a sample. Please call our European Support office toll-free at 00800 4573 8000, email [email protected], or contact us by live chat.

Answered by: Technical Support

Date published: 2017-05-18

What application is the blocking peptide sc-365422 P appropriate for?

Asked by: Germaine

Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization

Answered by: Technical Support

Date published: 2017-02-24

Rated 5 out of 5 by ganyidi from great for western blotThis antibody worked great for western blot, I get clear bands by using 1:600.

Date published: 2017-02-25

Rated 5 out of 5 by li65753 from Antibody is really great.Antibody is really great,it is being used for blotting or staining.

Date published: 2017-02-13

Rated 5 out of 5 by meetyi from Great antibody for WBThis antibody is really great. In the experiment, the analysis results of Western blot were very strong and clear, and the baseband is perfect.I suggest dilution this antibody at 1:200.

Date published: 2017-01-26

Rated 5 out of 5 by Professor Griffin from Specific and clearG alpha i-3 Antibody (H-7) produced clean and specific bands in multiple samples, and IF results were excellent

Date published: 2017-01-25

Rated 5 out of 5 by Wuuzi from GoodGood for western blot with human cell lines, worked wonderfully in our WB, Used at 1:1000.

Date published: 2017-01-12

Rated 5 out of 5 by MS from Good specificity in WB in HeLa whole cell lysateGood specificity in WB in HeLa whole cell lysate. -SCBT QC

Date published: 2015-01-26

Rated 5 out of 5 by MS from Strong cytoplasmic localization in formalinStrong cytoplasmic localization in formalin-fixed HepG2 cells in IF. -SCBT QC

Date published: 2015-01-12

Rated 5 out of 5 by HF from Good staining in IHC in retina and the outerGood staining in IHC in retina and the outer ears of mice. -SCBT Publication Review

Date published: 2013-08-20

Gα i-3抗体 (H-7) is rated 5.0 out of 5 by 8.

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种属	基因名称	基因ID	染色体位置	亚型（mRNA）登录号	蛋白质登录号	OMIM™ 号码
Human	GNAI2	Entrez Gene ID: 2771	3p21.31	NM_001166425, NM_001282617, NM_001282618, NM_001282619, NM_001282620, NM_002070	UniProt ID: P04899	192605
Human	GNAI3	Entrez Gene ID: 2773	1p13.3	NM_006496	UniProt ID: P08754	139370
Human	GNAI1	Entrez Gene ID: 2770	7q21.11	NM_001256414, NM_002069	UniProt ID: P63096	139310
Mouse	Gnai3	Entrez Gene ID: 14679	3 F2.3	NM_010306	UniProt ID: Q9DC51	N/A
Mouse	Gnai1	Entrez Gene ID: 14677	5 A3	NM_010305	UniProt ID: B2RSH2	N/A

G_{α i-3} 抗体 (H-7): sc-365422

G_{α i-3} 抗体 (H-7) 参考文献:

G_{α i-3} 抗体 (H-7) 规格

替代产品名称: G protein subunit GNAI3

产品克隆名称: H-7

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgG₃ kappa light chain

氨基酸: 339-354

抗原表位靶标: Gα i-3

抗原表位种属: rat

产品反应性/检测: G_{α i-1} and G_{α i-3}

种属反应性/检测: mouse, rat, human

其它哺乳动物反应性: equine, canine, bovine, porcine and avian

其它非哺乳动物反应性: N/A

应用: WB, IP, IF, ELISA

瓶的信息: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文: 14篇各种科学出版物的引文

G_{α i-3} 抗体 (H-7)可选的偶联物/形式: AC: Agarose, AF488: Alexa Fluor^® 488, AF647: Alexa Fluor^® 647, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

優選的 G_{α i-2} 抗體是 G_{α i-2} 抗體 (L5): sc-13534.

G_{α i-3} 抗体 (H-7)

G_{α i-3} (H-7): m-IgGκ BP-HRP 套装

G_{α i-3} 抗体 (H-7) AC

G_{α i-3} 抗体 (H-7) Alexa Fluor^® 488

G_{α i-3} 抗体 (H-7) Alexa Fluor^® 647

G_{α i-3} 抗体 (H-7) FITC

G_{α i-3} 抗体 (H-7) HRP

Gα i-3 (H-7) 中和勝肽

G_{α i-3} 抗体 (H-7) PE

Does this antibody work well in Raw 264.7 and Jurkat cell lines?

How likely is it that this antibody will show a cross reaction to Gnai2 since they are similar in structure? Was this tested? How similar is the immunogen the antibody was raised against to Gnai2? Thanks in advance!

What application is the blocking peptide sc-365422 P appropriate for?

Gα i-3 抗体 (H-7): sc-365422

Gα i-3 抗体 (H-7) 参考文献:

Gα i-3 抗体 (H-7) 规格

替代产品名称: G protein subunit GNAI3

产品克隆名称: H-7

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgG3 kappa light chain

氨基酸: 339-354

抗原表位靶标: Gα i-3

抗原表位种属: rat

产品反应性/检测: Gα i-1 and Gα i-3

种属反应性/检测: mouse, rat, human

其它哺乳动物反应性: equine, canine, bovine, porcine and avian

其它非哺乳动物反应性: N/A

应用: WB, IP, IF, ELISA

瓶的信息: Each vial contains 200 µg IgG3 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文: 14篇各种科学出版物的引文

Gα i-3 抗体 (H-7)可选的偶联物/形式: AC: Agarose, AF488: Alexa Fluor® 488, AF647: Alexa Fluor® 647, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

優選的 Gα i-2 抗體是 Gα i-2 抗體 (L5): sc-13534.

Gα i-3 抗体 (H-7)

Gα i-3 (H-7): m-IgGκ BP-HRP 套装

Gα i-3 抗体 (H-7) AC

Gα i-3 抗体 (H-7) Alexa Fluor® 488

Gα i-3 抗体 (H-7) Alexa Fluor® 647

Gα i-3 抗体 (H-7) FITC

Gα i-3 抗体 (H-7) HRP

Gα i-3 (H-7) 中和勝肽

Gα i-3 抗体 (H-7) PE

Does this antibody work well in Raw 264.7 and Jurkat cell lines?

How likely is it that this antibody will show a cross reaction to Gnai2 since they are similar in structure? Was this tested? How similar is the immunogen the antibody was raised against to Gnai2? Thanks in advance!

What application is the blocking peptide sc-365422 P appropriate for?

G_{α i-3} 抗体 (H-7): sc-365422

G_{α i-3} 抗体 (H-7) 参考文献:

G_{α i-3} 抗体 (H-7) 规格

抗体同型: IgG₃ kappa light chain

产品反应性/检测: G_{α i-1} and G_{α i-3}

瓶的信息: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

G_{α i-3} 抗体 (H-7)可选的偶联物/形式: AC: Agarose, AF488: Alexa Fluor^® 488, AF647: Alexa Fluor^® 647, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

優選的 G_{α i-2} 抗體是 G_{α i-2} 抗體 (L5): sc-13534.

G_{α i-3} 抗体 (H-7)

G_{α i-3} (H-7): m-IgGκ BP-HRP 套装

G_{α i-3} 抗体 (H-7) AC

G_{α i-3} 抗体 (H-7) Alexa Fluor^® 488

G_{α i-3} 抗体 (H-7) Alexa Fluor^® 647

G_{α i-3} 抗体 (H-7) FITC

G_{α i-3} 抗体 (H-7) HRP

G_{α i-3} 抗体 (H-7) PE