Date published: 2025-9-7

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Gα i-1/GNAI1 抗体 (SPM397): sc-56536

4.4(5)
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说明书
  • Gα i-1/GNAI1抗体(SPM397)是小鼠单克隆IgG2b κ, 在13篇文献中引用,规格为200 µg/ml
  • 针对rat来源的部分纯化的Gα i-1
  • 推荐用于 mouse, rat, human 和 bovine 来源的Gα i-1 WB 和 IP检测; 不会与 transducin, G α 0, G α i2, G α i3, or G α s产生交联反应
  • 看 Gα i-1/GNAI1 (R4): sc-13533,可查看有标记的 Gα i-1/GNAI1 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 目前,我们还没有完成Gα i-1/GNAI1 抗体 (SPM397)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

Gα i-1抗体(SPM397)是一种小鼠单克隆IgG2b抗体,可通过蛋白质印迹(WB)和免疫沉淀(IP)检测小鼠、大鼠、人类和牛的Gα i-1。Gα i-1是G蛋白α亚基Gi类中的关键成员,通过抑制腺苷酸环化酶活性从而降低细胞内环磷酸腺苷水平,对细胞信号传导发挥重要作用。这种对环磷酸腺苷的调节对于调节许多生理过程至关重要,包括心脏功能、神经递质释放和激素信号。Gα i-1主要位于质膜中,抗Gα i-1抗体(SPM397)可以检测其与G蛋白偶联受体(GPCR)的相互作用,后者将信号从细胞外部传递到内部,影响下游效应器和细胞反应。Gα i-1结合和分解GTP的能力对于其功能至关重要,可及时激活和关闭信号通路,确保细胞反应得到适当调节。Gα i-1与特定受体和下游效应子的相互作用对于维持细胞内稳态和应对环境变化至关重要,因此Gα i-1(SPM397)单克隆抗体成为药理学和神经生物学研究的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

Gα i-1/GNAI1 抗体 (SPM397)

sc-56536
200 µg/ml
RMB2377.00

What is the recommended fixation method for immunofluorescence staining with Gα i-1 (SPM397): sc-56536 monoclonal antibody?

Asked by: Germaine
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Excellent!This antibody is recommended for western blot analysis of Gα i-1 expression in SK-N-SH whole cell lysate and rat brain and mouse brain tissue extracts.
Date published: 2017-02-10
Rated 4 out of 5 by from Cytoplasmic and membrane staining observedCytoplasmic and membrane staining observed by immunofluorescence of formalin fixed HepG2 cells. -SCBT QC
Date published: 2015-05-07
Rated 5 out of 5 by from Publishable data obtained by Western BlotPublishable data obtained by Western Blot using human A2780 and OVCAR3 cells. -SCBT Publication Review
Date published: 2015-05-04
Rated 4 out of 5 by from Light backgroundLight background, but crisp solid band observed by Western Blot using SK-N-SH whole cell lysate.
Date published: 2015-01-22
Rated 4 out of 5 by from Different expression levels of the G iDifferent expression levels of the G i-1 protein demonstrated by Western Blot analysis using SK-N-SH whole cell lysate, rat and mouse brain tissue extracts.
Date published: 2014-11-17
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Gα i-1/GNAI1 抗体 (SPM397) is rated 4.4 out of 5 by 5.
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