Date published: 2025-9-8

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Gα 12 抗体 (B-5): sc-515610

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说明书
  • Gα 12抗体(B-5)是小鼠单克隆IgG2a κ, 在1篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于mouse物种的Gα 12的N-terminus的氨基酸2-27之间
  • 推荐用于 mouse, rat 和 human 来源的Gα 12 WB, IP, IF 和 ELISA检测
  • m-IgG Fc BP-HRPm-IgGκ BP-HRP是Gα 12 Antibody (B-5) 适用于 WB 应用。 的首选辅助检测试剂,这些试剂现与Gα 12 Antibody (B-5) 打包提供(请参阅下面的订购信息)。

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関連項目

Gα 12抗体(B-5)是一种小鼠单克隆IgG2a抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和酶联免疫吸附测定(ELISA)等方法检测小鼠、大鼠和人类样本中的Gα 12。Gα 12是异三聚体G蛋白家族的一员,在将信号从细胞表面受体传递到各种细胞内效应器方面发挥着至关重要的作用,从而影响许多生理过程。Gα 12单克隆抗体(B-5)有助于研究人员研究控制细胞增殖、迁移和分化的途径,使Gα 12成为正常细胞功能和发育所必需的。Gα 12主要位于细胞膜上,与各种受体和下游信号分子相互作用,促进细胞外信号转化为细胞反应。这种定位使Gα 12能够有效地将来自各种刺激的信息(包括激素和神经递质)传递给细胞内效应器,例如Rho鸟嘌呤核苷酸交换因子,后者随后激活Rho GTP酶。Gα 12能够调节这些通路,这凸显了其在维持细胞稳态方面的重要性,以及其在包括癌症和心血管疾病在内的各种疾病中的潜在影响。对于旨在探索Gα 12介导的复杂信号传导机制及其在健康和疾病中的作用的研究人员来说,抗Gα 12抗体(B-5)是一种宝贵的工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Gα 12 抗体 (B-5) 参考文献:

  1. Galpha12和Galpha13作为信号转导中的关键调节介质。  |  Kurose, H. 2003. Life Sci. 74: 155-61. PMID: 14607242
  2. G蛋白家族致癌基因Galpha(12)和Galpha(13)上调转化生长因子-β1基因。  |  Lee, SJ., et al. 2009. Oncogene. 28: 1230-40. PMID: 19151758
  3. G蛋白α12(Gα12)是肾损伤分子-1介导的渗出的负调控因子。  |  Ismail, OZ., et al. 2016. Am J Physiol Renal Physiol. 310: F607-F620. PMID: 26697979
  4. Gα12 通过调节 NFATc1 的表达来调控破骨细胞的生成。  |  Song, MK., et al. 2018. J Cell Mol Med. 22: 849-860. PMID: 29077264
  5. Gα12缺失通过抑制USP22/SIRT1调节的线粒体呼吸,加剧了肝脂肪化和肥胖。  |  Kim, TH., et al. 2018. J Clin Invest. 128: 5587-5602. PMID: 30300140
  6. G 蛋白 Gα12 和 Gα13 之间的受体选择性是由一个亮氨酸到异亮氨酸的变异决定的。  |  Mackenzie, AE., et al. 2019. FASEB J. 33: 5005-5017. PMID: 30601679
  7. 下调脑Gα12可减轻血管紧张素II依赖性高血压。  |  Gao, J., et al. 2020. Am J Hypertens. 33: 198-204. PMID: 31677381
  8. Gα12/13蛋白在实体瘤癌症特征中发挥的新作用。  |  Rasheed, SAK., et al. 2022. Oncogene. 41: 147-158. PMID: 34689178
  9. 对溶血磷脂酰丝氨酸识别和Gα12/13-P2Y10偶联特异性的见解。  |  Yin, H., et al. 2024. Cell Chem Biol. 31: 1899-1908.e5. PMID: 39265572
  10. 突触Gα12/13信号传导建立海马PV抑制回路。  |  Garbett, K., et al. 2024. Proc Natl Acad Sci U S A. 121: e2407828121. PMID: 39693341

订购信息

产品名称产品编号规格价格数量收藏夹

Gα 12 抗体 (B-5)

sc-515610
200 µg/ml
RMB2377.00

Gα 12 (B-5): m-IgG Fc BP-HRP 套装

sc-538806
200 µg Ab; 10 µg BP
RMB2662.00

Gα 12 (B-5): m-IgGκ BP-HRP 套装

sc-536480
200 µg Ab; 40 µg BP
RMB2662.00

Gα 12 (B-5) 中和勝肽

sc-515610 P
100 µg/0.5 ml
RMB511.00

What application is the blocking peptide sc-515610 P appropriate for?

Asked by: jerojero
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-28
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Rated 4 out of 5 by from A good antibody but with caution!!The G-alpha-12 is like a dizygotic twin of G-alpha 13, SCBT has excellent G-alpha 13 antibody (SC293424, please read my review), The G-alpha 12 fulfill the promise of not showing any corresponding band in the knockout cell line (see the gel picture) but unfortunately, it picks up a strong high molecular (>250kda) band. Now, the good news is that you can always cut your blot at that region and get rid of that non specific band and save the exhaustion of antibody. The non specific band may also be tissue/cell line specific, may not appear in your tissue of interest. This gene is relatively less expressed (when compared to G-alpha 13) in HEK293 cells. By the way,the extra band is not coming from Porin, because I checked that possibility, trust me! so, no worries, this is still good antibody and will do its job.
Date published: 2017-02-13
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G α 12 抗体 (B-5) is rated 4.0 out of 5 by 1.
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