Date published: 2025-9-7
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galectin-7 Antibody (G-3) - Western Blotting - Image 325100
galectin-7 抗体 (G-3): sc-137085. 人宫颈组织提取物 (A) 和 SCC-4 全细胞裂解液 (B) 中 galectin-7 表达的 Western 印迹分析.
galectin-7 抗体 (G-3): sc-137085. 人宫颈组织提取物 (A) 和 SCC-4 全细胞裂解液 (B) 中 galectin-7 表达的 Western 印迹分析.

galectin-7 抗体 (G-3): sc-137085

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说明书
  • galectin-7 抗体 G-3 是小鼠单克隆 IgG1 κ，galectin-7抗体, 在5篇文献中引用，规格为200 µg/ml
  • 针对氨基酸77-136，在galectin-7的human起源的C-terminus处进行映射
  • galectin-7 抗体 (G-3) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 galectin-7
  • 抗galectin-7抗体（G-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取galectin-7 (G-3): sc-137085的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 galectin-7 抗体 (G-3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与galectin-7 抗体 (G-3) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 galectin 抗体包括 galectin-1, galectin-10, galectin-2, galectin-3, galectin-4, galectin-5, galectin-7 和 galectin-8

galectin-7抗体（G-3）是一种IgG1 κ小鼠单克隆galectin-7抗体（也称为LGALS7抗体、Galectin-7抗体或Gal-7抗体），它通过WB、IP、IF、IHC(P)和ELISA等方法检测人类的galectin-7蛋白。galectin-7抗体（G-3）既可作为非共轭的抗galectin-7抗体形式提供，也可作为多种共轭形式的抗galectin-7抗体提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。半乳糖凝集素是一种可溶性β-半乳糖苷结合动物凝集素家族，可调节细胞与细胞间的粘附和细胞与细胞外基质（ECM）的相互作用，并在肿瘤进展、前mRNA剪接和凋亡中发挥作用。Galectin-7主要表达于分层鳞状上皮，被p53激活，被维甲酸抑制。它是一种促凋亡蛋白，在JNK激活和细胞色素c释放的上游在细胞内发挥作用。galectin-7基因定位于19号染色体。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

galectin-7 抗体 (G-3) 参考文献:

  1. Galectin-7 的过表达与紫外线诱导的晒伤角质细胞的凋亡过程缔合作用。  |  Bernerd, F., et al. 1999. Proc Natl Acad Sci U S A. 96: 11329-34. PMID: 10500176
  2. Galectin-7 (PIG1) 通过激活 JNK 和释放线粒体细胞色素 c 发挥促凋亡功能。  |  Kuwabara, I., et al. 2002. J Biol Chem. 277: 3487-97. PMID: 11706006
  3. Galectins-3 和 -7（而非 galectin-1）在伤口的再上皮化过程中发挥作用。  |  Cao, Z., et al. 2002. J Biol Chem. 277: 42299-305. PMID: 12194966
  4. 甲状腺肿瘤恶性发展过程中 galectin-7 和细胞角蛋白-19 表达的变化：诊断和生物学意义。  |  Rorive, S., et al. 2002. Mod Pathol. 15: 1294-301. PMID: 12481010
  5. 作为角膜上皮细胞迁移潜在介质的 Galectin-7  |  Cao, Z., et al. 2003. Arch Ophthalmol. 121: 82-6. PMID: 12523890
  6. 小鼠淋巴瘤细胞中 galectin-7 的上调与向侵袭性表型发展缔合作用。  |  Moisan, S., et al. 2003. Leukemia. 17: 751-9. PMID: 12682633
  7. 人 14 kDa β-半乳糖苷结合凝集素全长 cDNA 的克隆和核苷酸序列。  |  Hirabayashi, J., et al. 1989. Biochim Biophys Acta. 1008: 85-91. PMID: 2719964
  8. 人类 14-kDa 凝集素的分子克隆, 表征和表达。  |  Couraud, PO., et al. 1989. J Biol Chem. 264: 1310-6. PMID: 2910856
  9. 人类 galectin-7 的克隆, 表达和染色体图谱。  |  Madsen, P., et al. 1995. J Biol Chem. 270: 5823-9. PMID: 7534301
  10. Galectin-7是一种人类14-kDa S-选择蛋白，特异性表达于角质形成细胞，对维甲酸敏感。  |  Magnaldo, T., et al. 1995. Dev Biol. 168: 259-71. PMID: 7729568
  11. Galectin-7 是所有类型分层上皮细胞的标记。  |  Magnaldo, T., et al. 1998. Differentiation. 63: 159-68. PMID: 9697310
  12. 人类 galectin-7 识别碳水化合物的结构基础。  |  Leonidas, DD., et al. 1998. Biochemistry. 37: 13930-40. PMID: 9760227

订购信息

产品名称产品编号规格价格数量收藏夹

galectin-7 抗体 (G-3)

sc-137085
200 µg/ml
RMB2377.00

galectin-7 (G-3): m-IgG Fc BP-HRP 套装

sc-528857
200 µg Ab; 10 µg BP
RMB2662.00

galectin-7 (G-3): m-IgGκ BP-HRP 套装

sc-521422
200 µg Ab, 40 µg BP
RMB2662.00

galectin-7 (G-3): m-IgG1 BP-HRP 套装

sc-543171
200 µg Ab; 20 µg BP
RMB2662.00

galectin-7 抗体 (G-3) AC

sc-137085 AC
500 µg/ml, 25% agarose
RMB3129.00

galectin-7 抗体 (G-3) HRP

sc-137085 HRP
200 µg/ml
RMB2377.00

galectin-7 抗体 (G-3) FITC

sc-137085 FITC
200 µg/ml
RMB2482.00

galectin-7 抗体 (G-3) PE

sc-137085 PE
200 µg/ml
RMB2580.00

galectin-7 抗体 (G-3) Alexa Fluor® 488

sc-137085 AF488
200 µg/ml
RMB2685.00

galectin-7 抗体 (G-3) Alexa Fluor® 546

sc-137085 AF546
200 µg/ml
RMB2685.00

galectin-7 抗体 (G-3) Alexa Fluor® 594

sc-137085 AF594
200 µg/ml
RMB2685.00

galectin-7 抗体 (G-3) Alexa Fluor® 647

sc-137085 AF647
200 µg/ml
RMB2685.00

galectin-7 抗体 (G-3) Alexa Fluor® 680

sc-137085 AF680
200 µg/ml
RMB2685.00

galectin-7 抗体 (G-3) Alexa Fluor® 790

sc-137085 AF790
200 µg/ml
RMB2685.00

I am using galectin-7 (G-3): sc-137085 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Cweed
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2018-12-05
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Rated 3 out of 5 by from TestedMouse mAb against human Galectin-7 (G-3) was tested for reactivity with mouse Galectin-7. While the antibody showed a band corresponding to 14 kD with mouse lung tissue lysates, no positive reactivity was detected by immunofluorescent staining or by immunohistology.
Date published: 2018-02-27
Rated 5 out of 5 by from great antibodyi received a sample from santacruz to try. very good antibody for wb and imuunoflourescence
Date published: 2018-01-02
Rated 5 out of 5 by from ImmunofluorescenceI have got my data by using this antibody, and It is also easy to get a beautiful band
Date published: 2017-03-16
Rated 5 out of 5 by from Excellent immunoperoxidase nuclear and cytoplasmicExcellent immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human cervix tissue. -SCBT QC
Date published: 2015-05-13
Rated 5 out of 5 by from Published data for WB in human lymph and plasmaPublished data for WB in human lymph and plasma. -SCBT Publication Review
Date published: 2015-05-13
Rated 5 out of 5 by from VeryVery, robust band in WB in human cervix tissue extract and SCC-4 whole cell lysate. -SCBT QC
Date published: 2015-04-23
Rated 5 out of 5 by from GoodGood, strong band obtained in WB in human galectin-7 transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-03-24
Rated 5 out of 5 by from Published IHC(P) data for use in human SiHa and CPublished IHC(P) data for use in human SiHa and C-33A human cervical squamous carcinoma cell lines. -SCBT Publication Review
Date published: 2015-01-11
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galectin-7 抗体 (G-3) is rated 4.8 out of 5 by 8.
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