Date published: 2025-9-7

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galectin-4 抗体 (E-2): sc-271209

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说明书
  • galectin-4抗体(E-2)是小鼠单克隆IgG1 (kappa轻链),规格为200 µg/ml
  • 特异性抗原位于mouse物种的galectin-4的内部区域内的氨基酸129-187之间
  • galectin-4 抗体 (E-2) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测mouse 来源的 galectin-4
  • 抗galectin-4抗体(E-2)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取galectin-4 (E-2): sc-271209免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 galectin-4 抗体 (E-2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与galectin-4 抗体 (E-2) 组合成套装提供。 (请参阅下面的订购信息)。

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    関連項目

    半乳凝素-4抗体(E-2)是一种IgG1κ小鼠单克隆半乳凝素-4抗体,可通过WB、IP、IF、IHC(P)和ELISA检测小鼠来源的半乳凝素-4。半乳凝素-4 抗体 (E-2) 可作为非偶联的抗半乳凝素-4 抗体形式,以及多种偶联形式的抗半乳凝素-4 抗体,包括琼脂糖、HRP、PE、FITC 和多种 Alexa Fluor® 偶联物。半乳凝素是一类可溶性b-半乳糖苷结合的动物凝集素,可调节细胞间粘附和细胞与细胞外基质(ECM)的相互作用,并在肿瘤进展、mRNA前体剪接和细胞凋亡中发挥作用。这个家族的一个成员,半乳凝素-4,也被称为Gal-4、L36或LGALS4,位于人类染色体19q13.2。半乳凝素-4蛋白由323个氨基酸组成,含有两个同源的碳水化合物识别结构域(CRD)和半乳凝素家族中通常保守的所有氨基酸。半乳凝素-4的表达与人肝细胞癌(HCC)的恶性潜能相关,并且根据细胞间接触、血清生长因子、细胞生长和细胞分化状态而受到不同调节。半乳凝素-4在结肠、直肠和肠的上皮细胞以及HT29和LS174T细胞系中表达。半乳糖凝集素-4在结直肠癌癌症中表达不足,在易于腹膜扩散的细胞中优先上调。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    galectin-4 抗体 (E-2) 参考文献:

    1. 人类肝细胞癌变相关基因的鉴定和特征描述。  |  Kondoh, N., et al. 1999. Cancer Res. 59: 4990-6. PMID: 10519413
    2. 具有向腹膜或淋巴结高度转移潜能的糜烂性胃癌细胞的差异基因表达谱。  |  Hippo, Y., et al. 2001. Cancer Res. 61: 889-95. PMID: 11221876
    3. 肝内胆管癌中内源性 galectin-1 和 galectin-3 的表达。  |  Shimonishi, T., et al. 2001. Hum Pathol. 32: 302-10. PMID: 11274640
    4. 半乳糖凝集素-4在生理和疾病中的作用。  |  Cao, ZQ. and Guo, XL. 2016. Protein Cell. 7: 314-24. PMID: 27017379
    5. 人类 14-kDa 凝集素的分子克隆, 表征和表达。  |  Couraud, PO., et al. 1989. J Biol Chem. 264: 1310-6. PMID: 2910856
    6. 半乳糖凝集素-4缺乏小鼠的正常皮质髓鞘化。  |  Brocca, ME., et al. 2022. Cells. 11: PMID: 36359880
    7. 胰腺癌中,胞外凝集素-4可促进免疫逃逸并促进T细胞凋亡。  |  Lidström, T., et al. 2023. Cancer Immunol Res. 11: 72-92. PMID: 36478037
    8. 胞浆凝集素-4可连接细菌,限制其运动,并促进肠上皮细胞中炎症小体的激活。  |  Li, CS., et al. 2023. Proc Natl Acad Sci U S A. 120: e2207091120. PMID: 36689650
    9. 小鼠和人类中风后血浆半乳糖凝集素-4水平升高。  |  Jujic, A., et al. 2023. Int J Mol Sci. 24: PMID: 37373212
    10. 粘连连接蛋白是乳糖结合凝集素家族的成员。  |  Chiu, ML., et al. 1994. J Biol Chem. 269: 31770-6. PMID: 7989350
    11. 克隆和表达结直肠癌中下调的 S 型凝集素人 galectin-4 的 mRNA。  |  Rechreche, H., et al. 1997. Eur J Biochem. 248: 225-30. PMID: 9310382
    12. Galectin-4 和 galectin-6 是在小鼠胃肠道中表达的两种密切相关的凝集素。  |  Gitt, MA., et al. 1998. J Biol Chem. 273: 2954-60. PMID: 9446608

    订购信息

    产品名称产品编号规格价格数量收藏夹

    galectin-4 抗体 (E-2)

    sc-271209
    200 µg/ml
    RMB2377.00

    galectin-4 (E-2): m-IgG Fc BP-HRP 套装

    sc-529140
    200 µg Ab; 10 µg BP
    RMB2662.00

    galectin-4 (E-2): m-IgGκ BP-HRP 套装

    sc-521878
    200 µg Ab, 40 µg BP
    RMB2662.00

    galectin-4 (E-2): m-IgG1 BP-HRP 套装

    sc-543346
    200 µg Ab; 20 µg BP
    RMB2662.00

    galectin-4 抗体 (E-2) AC

    sc-271209 AC
    500 µg/ml, 25% agarose
    RMB3129.00

    galectin-4 抗体 (E-2) HRP

    sc-271209 HRP
    200 µg/ml
    RMB2377.00

    galectin-4 抗体 (E-2) FITC

    sc-271209 FITC
    200 µg/ml
    RMB2482.00

    galectin-4 抗体 (E-2) PE

    sc-271209 PE
    200 µg/ml
    RMB2580.00

    galectin-4 抗体 (E-2) Alexa Fluor® 488

    sc-271209 AF488
    200 µg/ml
    RMB2685.00

    galectin-4 抗体 (E-2) Alexa Fluor® 546

    sc-271209 AF546
    200 µg/ml
    RMB2685.00

    galectin-4 抗体 (E-2) Alexa Fluor® 594

    sc-271209 AF594
    200 µg/ml
    RMB2685.00

    galectin-4 抗体 (E-2) Alexa Fluor® 647

    sc-271209 AF647
    200 µg/ml
    RMB2685.00

    galectin-4 抗体 (E-2) Alexa Fluor® 680

    sc-271209 AF680
    200 µg/ml
    RMB2685.00

    galectin-4 抗体 (E-2) Alexa Fluor® 790

    sc-271209 AF790
    200 µg/ml
    RMB2685.00

    galectin-4 (E-2) 中和勝肽

    sc-271209 P
    100 µg/0.5 ml
    RMB511.00

    Does the unmasking have to be performed with citrate or EDTA for IHC on paraffin-embedded tissues ?

    Asked by: ManB
    Thank you for your question. You can find our recommended protocol by following this link: https://www.scbt.com/de/resources/protocols/immunoperoxidase-staining. We recommend Heat treatment: Place slides in a container and cover with 10 mM sodium citrate buffer, pH 6.0 (sc-294091); or with 50 mM glycine-HCl buffer (sc-29096), pH 3.5, with 0.01% (w/v) EDTA (sc-29092). Heat at 95° C for 5 minutes. Top off with fresh buffer and heat at 95° C for 5 minutes (optimal incubation time may vary for each tissue type). Allow slides to cool in the buffer for approximately 20 minutes. Wash in deionized H2O three times for 2 minutes each. Aspirate excess liquid from slides.
    Answered by: Tech Support Europe
    Date published: 2019-11-22

    What application is the blocking peptide sc-271209 P appropriate for?

    Asked by: Randy McDonald
    Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
    Answered by: Technical Support
    Date published: 2017-02-28
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    Rated 5 out of 5 by from Satisfactory Western Blot data of galectinSatisfactory Western Blot data of galectin-4 expression in non-transfected and mouse galectin-4 transfected 293T whole cell lysates. -SCBT QC
    Date published: 2015-03-14
    Rated 5 out of 5 by from Nice Western Blot data of galectinNice Western Blot data of galectin-4 expression in mouse cerebellum and rat cerebellum tissue extracts. -SCBT QC
    Date published: 2013-06-10
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    galectin-4 抗体 (E-2) is rated 5.0 out of 5 by 2.
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