Date published: 2025-9-7
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galectin-3 Antibody (B2C10) - Western Blotting - Image 390792
galectin-3 抗体 (B2C10) Alexa Fluor® 546: sc-32790 AF546. T84 (A), A-375 (B) 和 MCF7 (C) 全细胞裂解液中 galectin-3 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂阻断: sc-516214.
galectin-3 抗体 (B2C10) Alexa Fluor® 546: sc-32790 AF546. T84 (A), A-375 (B) 和 MCF7 (C) 全细胞裂解液中 galectin-3 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂阻断: sc-516214.

galectin-3 抗体 (B2C10): sc-32790

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说明书
  • galectin-3 抗体 B2C10 是小鼠单克隆 IgG1 κ，galectin-3抗体, 在104篇文献中引用，规格为200 µg/ml
  • 针对human起源的重组Galectin-3，其表位在前18个氨基酸内
  • galectin-3 抗体 (B2C10) 推荐用于 WB, IP, IF, IHC(P) 和 FCM，检测mouse, rat 和human 来源的 galectin-3
  • 抗galectin-3抗体（B2C10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还提供无叠氮化物的中和剂，sc-32790 L，200 µg/0.1 ml
  • 关于如何获取galectin-3 (B2C10): sc-32790的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 galectin-3 抗体 (B2C10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与galectin-3 抗体 (B2C10) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外galectin抗体，包括galectin-1, galectin-10, galectin-2, galectin-3, galectin-4, galectin-5, galectin-7和galectin-8

galectin-3 抗体（B2C10）是一种小鼠单克隆 IgG1 kappa 轻链抗体，经过工程改造，可靶向 galectin-3，也称为 ac-2、hMac-2（人类 Mac-2）、GALBP（半乳糖结合蛋白）、CBP35（碳水化合物结合蛋白 35）和 LGALS3（凝集素，半乳糖苷结合，可溶性，3）。抗-半乳糖凝集素-3抗体（B2C10）是针对重组人半乳糖凝集素-3的，其表位位于前18个氨基酸内，建议使用免疫印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和流式细胞术（FCM）检测小鼠、大鼠和人类样本中的半乳糖凝集素-3。Galectin-3是可溶性β-半乳糖苷结合凝集素家族的一员，其特点是具有一个单一的碳水化合物识别域（CRD），该识别域对于与含半乳糖的糖复合物的结合至关重要。这种结构特征使甘露聚糖-3能够调节重要的生物过程，如细胞间粘附、细胞与细胞外基质（ECM）相互作用以及细胞信号通路。甘露聚糖-3能够形成低聚物并通过CRD与多种配体相互作用，从而影响多种细胞功能，包括肿瘤进展、转移、凋亡和前mRNA剪接。Galectin-3可以以单体和低聚物的形式存在，这对于调节免疫反应、炎症和癌细胞行为非常重要。Galectin-3在细胞内参与信号传导通路和RNA处理，在细胞外通过与ECM成分相互作用促进细胞粘附和迁移。Galectin-3在结肠和肠道上皮细胞、炎症性巨噬细胞、乳头状和滤泡状癌、肿瘤星形细胞以及某些B和T淋巴细胞中表达突出。上调的galectin-3表达与癌症进展和转移、甲状腺恶性肿瘤和克罗恩病密切相关，是包括Hurthle细胞腺瘤和癌在内的疾病的宝贵诊断标志物。半乳糖凝集素-3单克隆抗体（B2C10）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

galectin-3 抗体 (B2C10) 参考文献:

  1. 鉴定人类 Galectin-3 基因的一个内部基因，该基因有两个不同的重叠阅读框，但不编码 Galectin-3。  |  Guittaut, M., et al. 2001. J Biol Chem. 276: 2652-7. PMID: 11160123
  2. 肝内胆管癌中内源性 galectin-1 和 galectin-3 的表达。  |  Shimonishi, T., et al. 2001. Hum Pathol. 32: 302-10. PMID: 11274640
  3. galectin-3 在腺癌肝转移中的作用  |  Inufusa, H., et al. 2001. Int J Oncol. 19: 913-9. PMID: 11604988
  4. 作为微小浸润性滤泡性甲状腺癌手术前免疫细胞诊断标志物的Galectin-3。  |  Saggiorato, E., et al. 2001. J Clin Endocrinol Metab. 86: 5152-8. PMID: 11701669
  5. 通过噬菌体展示肽库鉴定克罗恩病患者体内的抗大肠粘蛋白-3 IgG 自身抗体。  |  Jensen-Jarolim, E., et al. 2001. J Clin Immunol. 21: 348-56. PMID: 11720007
  6. Galectin-3 的磷酸化是其抗凋亡功能和细胞周期停滞所必需的。  |  Yoshii, T., et al. 2002. J Biol Chem. 277: 6852-7. PMID: 11724777
  7. Galectin-3介导乳腺癌细胞对β-1整合素的内吞。  |  Furtak, V., et al. 2001. Biochem Biophys Res Commun. 289: 845-50. PMID: 11735123
  8. Hürthle细胞腺瘤和癌细胞对galectin-3的不同反应。  |  Nascimento, MC., et al. 2001. Endocr Pathol. 12: 275-9. PMID: 11740048
  9. 人结肠腺癌 T84 细胞中 galectin-3 和 galectin-4 的定位存在惊人差异。Galectin-4 定位于细胞粘附部位。  |  Huflejt, ME., et al. 1997. J Biol Chem. 272: 14294-303. PMID: 9162064

订购信息

产品名称产品编号规格价格数量收藏夹

galectin-3 抗体 (B2C10)

sc-32790
200 µg/ml
RMB2377.00

galectin-3 (B2C10): m-IgG Fc BP-HRP 套装

sc-528474
200 µg Ab; 10 µg BP
RMB2662.00

galectin-3 (B2C10): m-IgGκ BP-HRP 套装

sc-520863
200 µg Ab, 40 µg BP
RMB2662.00

galectin-3 (B2C10): m-IgG1 BP-HRP 套装

sc-542952
200 µg Ab; 20 µg BP
RMB2662.00

galectin-3 抗体 (B2C10) L

sc-32790 L
200 µg/0.1 ml
RMB2377.00

galectin-3 抗体 (B2C10) AC

sc-32790 AC
500 µg/ml, 25% agarose
RMB3129.00

galectin-3 抗体 (B2C10) HRP

sc-32790 HRP
200 µg/ml
RMB2377.00

galectin-3 抗体 (B2C10) FITC

sc-32790 FITC
200 µg/ml
RMB2482.00

galectin-3 抗体 (B2C10) PE

sc-32790 PE
200 µg/ml
RMB2580.00

galectin-3 抗体 (B2C10) Alexa Fluor® 488

sc-32790 AF488
200 µg/ml
RMB2685.00

galectin-3 抗体 (B2C10) Alexa Fluor® 546

sc-32790 AF546
200 µg/ml
RMB2685.00

galectin-3 抗体 (B2C10) Alexa Fluor® 594

sc-32790 AF594
200 µg/ml
RMB2685.00

galectin-3 抗体 (B2C10) Alexa Fluor® 647

sc-32790 AF647
200 µg/ml
RMB2685.00

galectin-3 抗体 (B2C10) Alexa Fluor® 680

sc-32790 AF680
200 µg/ml
RMB2685.00

galectin-3 抗体 (B2C10) Alexa Fluor® 790

sc-32790 AF790
200 µg/ml
RMB2685.00

Can this antibody be used for intraperitoneal injection in mice? Is there a recommended dosage?

Asked by: Anonymous
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-08-30

Can galectin-3 Antibody (B2C10): sc-32790 be used to stain 4% PFA fixed, Frozen section of Rat tissue with IF microscopy? Is any antigen retrieval needed in this case?

Asked by: murahman
Thank you for your question. Yes, this antibody is suitable for IF and IHC(P) applications with rat tissue. We always recommend antigen retrieval when fixing with PFA. After fixing the cells, wash in three changes of PBS. Then incubate for 3 minutes in a solution of 0.01% Triton X-100 in PBS. https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2019-01-26

中和抗体として使う場合の適用濃度を教えてください。

Asked by: Miya
Thank you for your question. Please refer to PMID: 8634249. Please feel free to contact me if I may be of further assistance. お問い合わせを頂きまして、誠にありがとうございました。ご質問につきまして、PMID 8634249を参照してください。 その他の不明点等がございましたら、またご連絡ください。
Answered by: Technical Support 8
Date published: 2017-04-12

Can galectin-3 Antibody (B2C10): sc-32790 be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, this galectin-3 antibody sc-32790 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 12
Date published: 2017-01-09
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Rated 3 out of 5 by from Good staining in Raw cellsGood immunofluorescence staining in Raw 264.7 cells.
Date published: 2024-02-07
Rated 3 out of 5 by from Not working on ZebrafishThe antibody works great on human sample. We have tried it also on Zebrafish using the following dilution 1:100 and 1:500, however no signal was detected
Date published: 2021-01-26
Rated 4 out of 5 by from Antibody Review WBI try a trial size sample for this antibody and it works very well for WB at 1:500 dilution in human cells lines lisate. Thanks!
Date published: 2018-11-06
Rated 4 out of 5 by from Antibody reviewThe antibody worked at 1:500 dilution in mouse tissue lysate.
Date published: 2018-06-14
Rated 5 out of 5 by from 清晰的条带我们在胰腺癌细胞组的wb中使用了这支抗体，有清晰的目的条带，未见杂带，背景清晰，是适合做wb的抗体。
Date published: 2018-04-02
Rated 5 out of 5 by from very good antibody for wb and ifim so happy that i recevied a free trial from santacruz.the natibody worked perfectly with wb and if
Date published: 2018-01-02
Rated 5 out of 5 by from Great antibodyIt worked well. It can detect human Gal3 as well as dog (MDCK cells) gal3.
Date published: 2017-10-20
Rated 5 out of 5 by from worked well for WbThe antibody worked well in the mouse samples and got a pretty good impression of our lab.
Date published: 2017-02-12
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galectin-3 抗体 (B2C10) is rated 4.6 out of 5 by 17.
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