Date published: 2025-9-6
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GAL4 [DBD] Antibody (RK5C1) - Western Blotting - Image 5673
GAL4（DBD 1-147）Western blot分析：sc-4050标签融合蛋白（20 ng）。测试的抗体包括GAL4 ［DBD］抗体（RK5C1）： sc-510 在 1.0 µg/ml（A）、0.1 µg/ml（B）和 0.01 µg/ml（C）浓度下测试，以及 GAL4（DBD）：sc-577 在 0.1 µg/ml（D）和 0.01 µg/ml（E）浓度下测试。
GAL4（DBD 1-147）Western blot分析：sc-4050标签融合蛋白（20 ng）。测试的抗体包括GAL4 ［DBD］抗体（RK5C1）： sc-510 在 1.0 µg/ml（A）、0.1 µg/ml（B）和 0.01 µg/ml（C）浓度下测试，以及 GAL4（DBD）：sc-577 在 0.1 µg/ml（D）和 0.01 µg/ml（E）浓度下测试。

GAL4 [DBD] 抗体 (RK5C1): sc-510

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寫評論提問

说明书
  • GAL4 [DBD] 抗体 RK5C1 是小鼠单克隆 IgG2a κ，GAL4 [DBD]抗体, 在466篇文献中引用，规格为200 µg/ml
  • 针对氨基酸94-147的重组GAL4
  • GAL4 [DBD] 抗体 (RK5C1) 推荐用于 WB 和 IP，检测 GAL4 DNA binding domain
  • 抗GAL4 [DBD]抗体（RK5C1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 另提供生物素偶联抗体，可用于WB、IHC(P)和ELISA实验
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-510 X, 200 µg/0.1 ml)
  • 关于如何获取GAL4 [DBD] (RK5C1): sc-510的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP是GAL4 [DBD] Antibody (RK5C1) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与GAL4 [DBD] Antibody (RK5C1) 搭配使用（请参阅下面的订购信息）。

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描述
说明书与实验方案
研究信息

関連項目

附加GAL4抗体，包括GAL4-TA

GAL4 [DBD]抗体（RK5C1）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）和免疫沉淀（IP）检测GAL4。抗GAL4抗体（RK5C1）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。GAL4源自酿酒酵母，是一种关键的转录激活因子，在基因表达调控中起着至关重要的作用。N端147个氨基酸残基对于特异性和牢固的DNA结合至关重要，尽管这些残基不能独立促进有效的转录激活。这一特性使GAL4成为分子生物学领域的重要工具，特别是用于创建将GAL4与来自各种转录调节剂的激活结构域结合的融合蛋白。这种融合蛋白可以有效地激活包括酵母、植物、昆虫和哺乳动物细胞在内的一系列生物体的转录。此外，GAL4在开发"双杂交"检测方法方面也发挥了重要作用，该方法使研究人员能够探索特定的蛋白质-蛋白质相互作用，从而加深我们对细胞过程和调节机制的理解。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

GAL4 [DBD] 抗体 (RK5C1) 参考文献:

  1. 用于在酵母或哺乳动物细胞中表达的 GAL4 融合载体。  |  Sadowski, I., et al. 1992. Gene. 118: 137-41. PMID: 1511877
  2. 双杂交系统：一种识别和克隆与感兴趣的蛋白质相互作用的蛋白质基因的方法。  |  Chien, CT., et al. 1991. Proc Natl Acad Sci U S A. 88: 9578-82. PMID: 1946372
  3. 酵母 STE12 蛋白的信息素依赖性磷酸化与转录激活有关。  |  Song, D., et al. 1991. Genes Dev. 5: 741-50. PMID: 2026326
  4. 激活剂和目标。  |  Ptashne, M. and Gann, AA. 1990. Nature. 346: 329-31. PMID: 2142753
  5. 检测蛋白质-蛋白质相互作用的新型基因系统。  |  Fields, S. and Song, O. 1989. Nature. 340: 245-6. PMID: 2547163
  6. 真菌基因调控机制模型：酿酒酵母的 GAL 基因。  |  Johnston, M. 1987. Microbiol Rev. 51: 458-76. PMID: 2830478
  7. GAL4 的缺失分析确定了两个转录激活区段。  |  Ma, J. and Ptashne, M. 1987. Cell. 48: 847-53. PMID: 3028647

订购信息

产品名称产品编号规格价格数量收藏夹

GAL4 [DBD] 抗体 (RK5C1)

sc-510
200 µg/ml
RMB2377.00

GAL4 [DBD] (RK5C1): m-IgG Fc BP-HRP 套装

sc-525422
200 µg Ab; 10 µg BP
RMB2662.00

GAL4 [DBD] 抗体 (RK5C1) X

sc-510 X
200 µg/0.1 ml
RMB2377.00

GAL4 [DBD] 抗体 (RK5C1) AC

sc-510 AC
500 µg/ml, 25% agarose
RMB3129.00

GAL4 [DBD] 抗体 (RK5C1) HRP

sc-510 HRP
200 µg/ml
RMB2377.00

GAL4 [DBD] 抗体 (RK5C1) FITC

sc-510 FITC
200 µg/ml
RMB2482.00

GAL4 [DBD] 抗体 (RK5C1) PE

sc-510 PE
200 µg/ml
RMB2580.00

GAL4 [DBD] 抗体 (RK5C1) Alexa Fluor® 488

sc-510 AF488
200 µg/ml
RMB2685.00

GAL4 [DBD] 抗体 (RK5C1) Alexa Fluor® 546

sc-510 AF546
200 µg/ml
RMB2685.00

GAL4 [DBD] 抗体 (RK5C1) Alexa Fluor® 594

sc-510 AF594
200 µg/ml
RMB2685.00

GAL4 [DBD] 抗体 (RK5C1) Alexa Fluor® 647

sc-510 AF647
200 µg/ml
RMB2685.00

GAL4 [DBD] 抗体 (RK5C1) Alexa Fluor® 680

sc-510 AF680
200 µg/ml
RMB2685.00

GAL4 [DBD] 抗体 (RK5C1) Alexa Fluor® 790

sc-510 AF790
200 µg/ml
RMB2685.00

GAL4 [DBD] 抗体 (RK5C1) B

sc-510 B
200 µg/ml
RMB2407.00

Does this antibody work for flow cytometry?

Asked by: ABLab
Thank you for your question. sc-510 GAL4 [DBD] Antibody (RK5C1) can be used for Flow cytometry,WB, IP,IF and IHC. Here is our flow cytometry protocol : https://www.scbt.com/resources/protocols/flow-cytometry
Answered by: BlakeJ
Date published: 2024-10-04

Hello. I would like to test a free sample of this antibody. Would you mind telling me how to proceed? Yours

Asked by: bfav
Thank you for your question. Please contact Technical Service or your local distributor for information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Tech Service
Date published: 2019-12-16

Does this antibody work in ICC/IHC?

Asked by: sciencebeetle
Thank you for your question. We have not yet tested this antibody for IF or IHC(P) and so these are not recommended applications at this time. However, we are aware of almost 20 different publications that cite use of the antibody for IF or IHC so we can extend our warranty to cover this application. Please contact Technical Service if you would like a list of the specific product citations, or use the product citation search on our website and you can search by application, species and antibody.
Answered by: Technical Support
Date published: 2018-12-14

Hello, Would it be possible to receive a free sample of this antibody?

Asked by: YuliaLampi
Thank you for your question. Please contact Technical Service for information on how to obtain a sample.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-14

Dear Sir/Madam, I would like to order a Gal4 antibody for immunohistochemistry in plant protoplast extracts. Would it be possible to receive a free sample of GAL4 (RK5C1): sc-510 to test it before buying it?

Asked by: Dmitri
Thank you for your question. Please contact Technical Service for more information on how to receive a 10 μg sample of our well characterized monoclonal antibody.
Answered by: Technical Support
Date published: 2018-12-05

Dear Sir/Madam, I would like to order a Gal4 antibody for immunohistochemistry in zebrafish embryos. Would it be possible to receive a free sample of GAL4 (RK5C1): sc-510 to test it before buying it? Many thanks, Florence GIGER, PhD.

Asked by: FloG
Thank you for the question. Yes, we will contact you by email to provide a FREE 10 µg sample of GAL4 (RK5C1): sc-510.
Answered by: Technical Support Europe
Date published: 2017-12-27

I am using this antibody to try to detect yeast two hybrid Gal4DBD-fusion protein expression and I have not been able to detect any even with a high copy vector. What is the WB protocol used for this antibody? Dissolved in BSA or Milk? What concentration?

Asked by: aloha
Thank you for your question. We recommend a starting dilution of 1:200 with this antibody, and we recommend diluting in 5% milk with TBS-T. Here is a link to the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting Please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Tech Service
Date published: 2017-12-08

Does this antibody work for Drosophila UAS/Gal4 system? Has anyone tried it?

Asked by: sameerbau
Thank you for your question. This monoclonal antibody, GAL4 (RK5C1): sc-510, is recommended for detection of GAL4 DNA binding domain, and is not species-specific. We are aware of several publications specifically using the antibody in a drosophila model. Please refer to the following publications on NCBI's PubMed as a reference, PMID's: 12819212, 10766736 and 12514098.
Answered by: Technical Support
Date published: 2017-06-27
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Rated 5 out of 5 by from Good antibody for immunoprecipitationI used this antibody for immunoprecipitation assay of transfected 293T cells and it worked very well. For 0.5 ml lysates I used 4 ul of antibody.
Date published: 2022-11-06
Rated 4 out of 5 by from good antibodyI use 1:200 in HEK293T transfected with GAL4 plasmid. It works very well.
Date published: 2020-08-25
Rated 5 out of 5 by from Gal4 antibody that works!we tried a free sample of this antibody since our invitrogen one did not seem to work on cell lysates
Date published: 2018-02-16
Rated 5 out of 5 by from Works both on WB and IPI have used this antibody before as agarose conjugate but this was my first time using it for both IP and WB. It works very well without any background. For WB I used it at 1:2000 in TBS O/N and for IP I used 2 ug for 250 ug of nuclear extracts.
Date published: 2017-08-27
Rated 5 out of 5 by from Excellent choiceHighly reliable antibody that is well cited in literature and performs cleanly, especially in Western Blot.
Date published: 2017-01-12
Rated 5 out of 5 by from Able to publish using the antibody in WBAble to publish using the antibody in WB in HEK293 cells and mouse 3T3-L1 cells.
Date published: 2015-09-23
Rated 4 out of 5 by from Strong band in WB and IHC in frican green monkeyStrong band in WB and IHC in frican green monkey, mouse, human COS-7, L cells, P19, HEK 293, 293T. -SCBT Publication Review
Date published: 2015-04-11
Rated 4 out of 5 by from Robust signal in WB in yeast recombinantRobust signal in WB in yeast recombinant GAL4 fusion protein. -SCBT QC
Date published: 2015-03-30
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GAL4 [DBD] 抗体 (RK5C1) is rated 4.5 out of 5 by 11.
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