Date published: 2025-9-6

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GABAA Rβ3 抗体 (D-12): sc-376252

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说明书
  • GABAA R beta 3 抗体 D-12 是小鼠单克隆 IgG1 κ,GABAA Rβ3抗体, 在7篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的GABAA Rβ3的C-terminus附近的氨基酸243-481之间
  • GABAA R beta 3 抗体 (D-12) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 GABAA Rβ3; 也和以下物种反应,包括: 和 equine, canine, bovine and porcine
  • 抗GABAA R beta 3抗体(D-12)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取GABAA Rβ3 (D-12): sc-376252免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是GABAA Rβ3 Antibody (D-12) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与GABAA Rβ3 Antibody (D-12) 打包提供(请参阅下面的订购信息)。

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関連項目

GABAA Rβ3抗体(D-12)是一种IgG1 κ小鼠单克隆GABAA Rβ3抗体(也称为GABAA Rβ3抗体),可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的GABAA Rβ3蛋白。GABAA Rβ3抗体(D-12)既有非偶联型抗GABAA Rβ3抗体形式,也有多种偶联型抗GABAA Rβ3抗体形式,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。GAD-65和GAD-67,即谷氨酸脱羧酶,主要起催化产生GABA(γ-氨基丁酸)的作用。在中枢神经系统中,GABA通过增加抑制神经元放电的Cl-电导,作为主要的抑制性递质发挥作用。已证实GABA可以激活离子型(GABAA)和代谢型(GABAB)受体,以及第三类称为GABAC的受体。GABAA和GABAC都是配体门控离子通道,但它们在结构和功能上有所不同。GABAA受体家族成员包括GABAA Rα1-6、GABAA R β1-3、GABAA Rγ1-3、GABAA Rδ、GABAA Rε、GABAA Rρ1和GABAA Rρ2。GABAB家族由GABAB R1α和GABAB R1β组成。目前已确定GABA转运蛋白包括GABA T-1、GABA T-2和GABA T-3(也称为GAT-1、-2和-3)。GABA转运蛋白的功能是终止GABA的作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

GABAA Rβ3 抗体 (D-12) 参考文献:

  1. γ-氨基丁酸(GABA)转运系统的分子异质性。从大鼠大脑中克隆出两种新型高亲和力 GABA 转运体。  |  Borden, LA., et al. 1992. J Biol Chem. 267: 21098-104. PMID: 1400419
  2. GABA:出生后早期的兴奋性递质。  |  Cherubini, E., et al. 1991. Trends Neurosci. 14: 515-9. PMID: 1726341
  3. 克隆人脑 GABA 转运体。  |  Nelson, H., et al. 1990. FEBS Lett. 269: 181-4. PMID: 2387399
  4. GABRB3多态性与精神分裂症的关系。  |  Liu, Y., et al. 2018. J Mol Neurosci. 64: 75-79. PMID: 29196882
  5. GABRB3变体的结构图谱揭示了基因型与表型之间的关联。  |  Johannesen, KM., et al. 2022. Genet Med. 24: 681-693. PMID: 34906499
  6. Gabrb3内皮细胞特异性基因敲除小鼠会出现血流异常、高血压和行为障碍。  |  Agrud, A., et al. 2022. Sci Rep. 12: 4922. PMID: 35318369
  7. GABAA受体基因变异(GABRA1、GABRB2、GABRB3、GABRG2)患者的分子和临床描述:队列研究、文献综述和基因型-表型关联。  |  Maillard, PY., et al. 2022. Epilepsia. 63: 2519-2533. PMID: 35718920
  8. Gabrb3是体感觉皮层锥体神经元亚型功能整合所必需的。  |  Babij, R., et al. 2023. Neuron. 111: 256-274.e10. PMID: 36446382
  9. 谷氨酸脱羧酶 67 kDa 异构体与谷氨酸脱羧酶 65 kDa 异构体的 NH2 端区域相互作用,从而使谷氨酸脱羧酶 67 kDa 异构体靶向细胞内器官。  |  Dirkx, R., et al. 1995. J Biol Chem. 270: 2241-6. PMID: 7836456
  10. 脊椎动物视网膜中的 GABAC 受体。  |  Lukasiewicz, PD. 1996. Mol Neurobiol. 12: 181-94. PMID: 8884747
  11. GABA(B)受体的表达克隆发现了其与代谢型谷氨酸受体的相似性。  |  Kaupmann, K., et al. 1997. Nature. 386: 239-46. PMID: 9069281
  12. GABA(A)受体亚型:苯并二氮杂卓部位配体的临床疗效和选择性。  |  Korpi, ER., et al. 1997. Ann Med. 29: 275-82. PMID: 9375983

订购信息

产品名称产品编号规格价格数量收藏夹

GABAA Rβ3 抗体 (D-12)

sc-376252
200 µg/ml
RMB2377.00

GABAA Rβ3 (D-12): m-IgGκ BP-HRP 套装

sc-522894
200 µg Ab, 40 µg BP
RMB2662.00

GABAA Rβ3 (D-12): m-IgG1 BP-HRP 套装

sc-551907
200 µg Ab; 20 µg BP
RMB2662.00

GABAA Rβ3 抗体 (D-12) AC

sc-376252 AC
500 µg/ml, 25% agarose
RMB3129.00

GABAA Rβ3 抗体 (D-12) HRP

sc-376252 HRP
200 µg/ml
RMB2377.00

GABAA Rβ3 抗体 (D-12) FITC

sc-376252 FITC
200 µg/ml
RMB2482.00

GABAA Rβ3 抗体 (D-12) PE

sc-376252 PE
200 µg/ml
RMB2580.00

GABAA Rβ3 抗体 (D-12) Alexa Fluor® 488

sc-376252 AF488
200 µg/ml
RMB2685.00

GABAA Rβ3 抗体 (D-12) Alexa Fluor® 546

sc-376252 AF546
200 µg/ml
RMB2685.00

GABAA Rβ3 抗体 (D-12) Alexa Fluor® 594

sc-376252 AF594
200 µg/ml
RMB2685.00

GABAA Rβ3 抗体 (D-12) Alexa Fluor® 647

sc-376252 AF647
200 µg/ml
RMB2685.00

GABAA Rβ3 抗体 (D-12) Alexa Fluor® 680

sc-376252 AF680
200 µg/ml
RMB2685.00

GABAA Rβ3 抗体 (D-12) Alexa Fluor® 790

sc-376252 AF790
200 µg/ml
RMB2685.00

GABAA Rβ3 (D-12) 中和勝肽

sc-376252 P
100 µg/0.5 ml
RMB511.00

Hello, I would like to know if you could you provide me the epitope sequence of this antibody? We would want to perform a sequence alignment with GABAA-R from insects, to test if this antibody would be useful to detect the receptor from these organisms.

Asked by: ivan_f
Thank you for your question. This mouse monoclonal antibody is specific for an epitope mapping between amino acids 243-481 near the C-terminus of GABAA Rβ3 of human origin (protein accession number P28472). Please contact our Technical Service Department if you would like our help performing sequence analysis against the corresponding protein in insects. Please call us toll-free at (800) 457-3801 option 2, e-mail <[email protected]>, or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-12-07

What application is the blocking peptide sc-376252 P appropriate for?

Asked by: Professor Griffin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Good featuresThe research resullts is good. I used the antibody at 1:1000 in Western Blot. The obtained protein bands are clear.
Date published: 2017-09-18
Rated 5 out of 5 by from Produced nice Western blot data of GABAAProduced nice Western blot data of GABAA R 3 expression in H4, SK-N-SH, IMR-32 and A549 whole cell lysates. -SCBT QC
Date published: 2013-07-03
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GABA A Rβ3 抗体 (D-12) is rated 5.0 out of 5 by 2.
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