Date published: 2025-9-7
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FUS/TLS Antibody (4H11) - Western Blotting - Image 370753
FUS/TLS 抗体 (4H11) HRP: sc-47711 HRP. c4 (A), NAMALWA (B), RAW 264. 7 (C), THP-1 (D), K-562 (E) 和 Jurkat (F) 全细胞裂解液中 FUS/TLS 表达的直接 Western 印迹分析.
FUS/TLS 抗体 (4H11) HRP: sc-47711 HRP. c4 (A), NAMALWA (B), RAW 264. 7 (C), THP-1 (D), K-562 (E) 和 Jurkat (F) 全细胞裂解液中 FUS/TLS 表达的直接 Western 印迹分析.

FUS/TLS 抗体 (4H11): sc-47711

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说明书
  • FUS/TLS 抗体 4H11 是小鼠单克隆 IgG1 κ，FUS/TLS抗体, 在160篇文献中引用，规格为200 µg/ml
  • 针对与humanTLS的C-terminus对应的融合蛋白
  • FUS/TLS 抗体 (4H11) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 FUS/TLS
  • 抗FUS/TLS抗体（4H11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取FUS/TLS (4H11): sc-47711的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 FUS/TLS 抗体 (4H11) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与FUS/TLS 抗体 (4H11) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

FUS/TLS抗体（4H11）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学检测小鼠、大鼠和人类来源的FUS/TLS蛋白。抗FUS/TLS抗体（4H11）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。FUS/TLS蛋白，也称为异质核糖核蛋白（hnRNP）复合物成分，在核内的RNA处理和运输中起着至关重要的作用。FUS/TLS蛋白通过与RNA结合并影响RNA的稳定性和定位来参与基因表达的调控。在细胞应激反应和癌症发展过程中，这种功能尤为重要，因为异常的RNA处理会导致致癌转化。FUS/TLS蛋白与多种核类固醇受体相互作用，表明其参与激素诱导的转录调控。此外，FUS/TLS蛋白还与染色体易位有关，可导致融合蛋白的产生，如EWS/Fli-1嵌合蛋白，该蛋白在尤因肉瘤中充当有效的转录激活因子。了解FUS/TLS蛋白在这些过程中的作用对于阐明其对正常细胞功能和疾病机制的贡献至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

FUS/TLS 抗体 (4H11) 参考文献:

  1. 人类肿瘤中染色体易位引起的带有 ETS DNA 结合域的基因融合。  |  Delattre, O., et al. 1992. Nature. 359: 162-5. PMID: 1522903
  2. 16号染色体上的FUS/TLS基因突变会导致家族性肌萎缩性侧索硬化症。  |  Kwiatkowski, TJ., et al. 2009. Science. 323: 1205-8. PMID: 19251627
  3. 成人和儿童恶性疾病肿瘤溶解综合征（TLS）风险评估和预防建议：TLS专家小组共识。  |  Cairo, MS., et al. 2010. Br J Haematol. 149: 578-86. PMID: 20331465
  4. 从DNA修复到应激反应：FUS/TLS的功能：对ALS的影响。  |  Sama, RR., et al. 2014. ASN Neuro. 6: PMID: 25289647
  5. 缺氧诱导的 FUS-circTBC1D14 应激颗粒促进 TNBC 的自噬。  |  Liu, Y., et al. 2023. Adv Sci (Weinh). 10: e2204988. PMID: 36806670
  6. 肺癌引流淋巴结会影响肿瘤内第三淋巴结构（TLS）的成熟。  |  He, M., et al. 2023. J Immunother Cancer. 11: PMID: 37072348
  7. 变异型尤文氏肉瘤易位（7;22）将 EWS 基因与 ETS 基因 ETV1 融合。  |  Jeon, IS., et al. 1995. Oncogene. 10: 1229-34. PMID: 7700648
  8. 与 FLI-1 相比，尤文氏肉瘤 EWS/FLI-1 融合基因编码一种更强的转录激活剂，是一种更强大的转化基因。  |  May, WA., et al. 1993. Mol Cell Biol. 13: 7393-8. PMID: 8246959
  9. 在人类肌样脂肪肉瘤中，CHOP 与一种新型 RNA 结合蛋白融合。  |  Crozat, A., et al. 1993. Nature. 363: 640-4. PMID: 8510758
  10. EWS-ATF-1基因涉及染色体t（12；22）易位的软组织恶性黑色素瘤，该基因编码一种组成型转录激活因子。  |  Fujimura, Y., et al. 1996. Oncogene. 12: 159-67. PMID: 8552387
  11. 在尤文肿瘤中与 EWS 融合的 ETS 家族新成员。  |  Peter, M., et al. 1997. Oncogene. 14: 1159-64. PMID: 9121764
  12. TLS（易位脂肪肉瘤）是类固醇, 甲状腺激素和视黄醇受体的高亲和性互作因子。  |  Powers, CA., et al. 1998. Mol Endocrinol. 12: 4-18. PMID: 9440806

订购信息

产品名称产品编号规格价格数量收藏夹

FUS/TLS 抗体 (4H11)

sc-47711
200 µg/ml
RMB2377.00

FUS/TLS (4H11): m-IgG Fc BP-HRP 套装

sc-528510
200 µg Ab; 10 µg BP
RMB2662.00

FUS/TLS (4H11): m-IgGκ BP-HRP 套装

sc-520905
200 µg Ab, 40 µg BP
RMB2662.00

FUS/TLS (4H11): m-IgG1 BP-HRP 套装

sc-542967
200 µg Ab; 20 µg BP
RMB2662.00

FUS/TLS 抗体 (4H11) AC

sc-47711 AC
500 µg/ml, 25% agarose
RMB3129.00

FUS/TLS 抗体 (4H11) HRP

sc-47711 HRP
200 µg/ml
RMB2377.00

FUS/TLS 抗体 (4H11) FITC

sc-47711 FITC
200 µg/ml
RMB2482.00

FUS/TLS 抗体 (4H11) PE

sc-47711 PE
200 µg/ml
RMB2580.00

FUS/TLS 抗体 (4H11) Alexa Fluor® 488

sc-47711 AF488
200 µg/ml
RMB2685.00

FUS/TLS 抗体 (4H11) Alexa Fluor® 546

sc-47711 AF546
200 µg/ml
RMB2685.00

FUS/TLS 抗体 (4H11) Alexa Fluor® 594

sc-47711 AF594
200 µg/ml
RMB2685.00

FUS/TLS 抗体 (4H11) Alexa Fluor® 647

sc-47711 AF647
200 µg/ml
RMB2685.00

FUS/TLS 抗体 (4H11) Alexa Fluor® 680

sc-47711 AF680
200 µg/ml
RMB2685.00

FUS/TLS 抗体 (4H11) Alexa Fluor® 790

sc-47711 AF790
200 µg/ml
RMB2685.00

I got very clean and strong signal when I carried out WB  in canis cells. Recently,I try to detect mRNA level of Fus ;however, Fus have different  transcript variant product. I would like to know this antibody bind to what kind of Fus isoform.Thanks.

Asked by: Johnliliy
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 option 2, allowing for a more interactive discussion of this and other related questions.
Answered by: Blake J
Date published: 2021-08-02

Is it possible to provide the exact FUS/TLS peptide sequence that was used as the epitope for the production of this antibody ?

Asked by: Max19
Thank you for your question. Unfortunately, the exact epitope recognized by this mouse monoclonal antibody, FUS/TLS (4H11): sc-47711, has not been determined by Santa Cruz Biotechnology.
Answered by: Technical Support
Date published: 2018-10-24

Does the FUS/TLS Antibody (4H11) react with bovine tissue samples?

Asked by: Trav11
For additional species reactivity, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website and we will be more than happy to run sequence analysis for your species of interest.
Answered by: Tech Service 8
Date published: 2016-12-28
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Rated 5 out of 5 by from Good for WB & ICHGood detection in WB & ICH for human cells and monkey cells.
Date published: 2024-11-05
Rated 5 out of 5 by from Functions as expected.The antibody was used for immunofluorescence with secondary anti-mouse antibody fused with Alexa488, in osteosarcoma cell lines U2OS. In all the cell lines tested, the signal was of expected localization and intensity, observed mostly as diffuse fluorescence in the nucleus and as foci in the cytoplasm upon induced hyperosmotic stress.
Date published: 2023-07-04
Rated 3 out of 5 by from Didn't work in Xenopus for IFI tried to use this antibody for IF in Xenopus RGC axons with a 1:100 dilution and I got a very low signal.
Date published: 2021-10-07
Rated 5 out of 5 by from Good for WBTested on human peripheral blood mononuclear cells and on a human neuroblastoma cell line. Good signal. Detected some faint bands suspected to be specific for FUS proteolysis
Date published: 2019-01-30
Rated 4 out of 5 by from Excellent for WBI got very clean and strong signal when I carried out WB experiment. Nice antibody!
Date published: 2018-04-25
Rated 4 out of 5 by from buon funzionamentoho testato questo anticorpo in western blot and immunofluorescenza in fibroblasti in coltura con risultati molto buoni. Nell'immagine allegata si può osservare l'effetto dell'anticorpo FUS su cellule 3T3 e fibroblasti umani. Inoltre, sugli gli stessi estratti è stato testato l'Ab ubiquitin (cod Santa Cruz: P4D1). Per questi tests abbiamo utilizzato un altro anticorpo Santa Cruz, il secondario m-IgGk BP-HRP. Questi tre anticorpi funzionano molto bene con un segnale pulito. Sia FUS 4H11 che Ubiquitin P4D1 funzionano anche molto bene in immunofliorescenza
Date published: 2017-11-08
Rated 4 out of 5 by from Good in WB and IHCUsed in transfected HeLa and mouse brain, observed good stainings
Date published: 2017-10-19
Rated 4 out of 5 by from Excellent for WB!It detected specific band for Fus in neural cells.
Date published: 2017-09-21
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FUS/TLS 抗体 (4H11) is rated 4.5 out of 5 by 17.
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