Date published: 2025-9-7

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FucT-VIII 抗体 (B-10): sc-271244

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说明书
  • FucT-VIII 抗体 B-10 是小鼠单克隆 IgG1 κ,FucT-VIII抗体, 在9篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列31-230位于human物种的FucT-VIII的N-terminus附近
  • FucT-VIII 抗体 (B-10) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 FucT-VIII isoforms 1 and 2
  • 抗FucT-VIII抗体(B-10)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取FucT-VIII (B-10): sc-271244免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是FucT-VIII Antibody (B-10) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与FucT-VIII Antibody (B-10) 打包提供(请参阅下面的订购信息)。

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関連項目

FucT-VIII抗体(B-10)是一种IgG1 κ小鼠单克隆FucT-VIII抗体(也称为FucT-VIII抗体),可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类的FucT-VIII蛋白。FucT-VIII抗体(B-10)既可以以非共轭的抗FucT-VIII抗体形式提供,也可以以多种共轭的抗FucT-VIII抗体形式提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。岩藻糖基转移酶催化岩藻糖与糖受体分子中不同位置键的共价结合。生成并共价连接到细胞表面的碳水化合物部分是必要的,以确保满足生理作用的表面轮廓,这依赖于选择素等粘附分子。造血谱系依靠岩藻糖基转移酶赋予表面碳水化合物表型,介导适当的细胞粘附分子募集和细胞迁移。α-(1,6)-岩藻糖基转移酶或岩藻糖基转移酶8(FucT-VIII)催化岩藻糖以α 1-6键连接到N-连接寡糖的最内层GlcNAc残基上。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

FucT-VIII 抗体 (B-10) 参考文献:

  1. 参与α1,6-岩藻糖基转移酶供体底物结合的序列基团:保守精氨酸残基的作用。  |  Takahashi, T., et al. 2000. Glycobiology. 10: 503-10. PMID: 10764839
  2. 新型人类 UDP-GalNAc:多肽 N-乙酰半乳糖氨酰基转移酶 GalNAc-T8 的分子克隆及作为常染色体显性低磷血症佝偻病(ADHR)候选基因的分析。  |  White, KE., et al. 2000. Gene. 246: 347-56. PMID: 10767557
  3. 人类α1,6-岩藻糖基转移酶基因(FUT8)的基因组结构和启动子分析。  |  Yamaguchi, Y., et al. 2000. Glycobiology. 10: 637-43. PMID: 10814706
  4. 编码α6-岩藻糖基转移酶的基因 FUT8 的祖先外显子组织显示了连续的肽结构域,这表明α6-岩藻糖基转移酶家族具有特定的三维核心结构。  |  Javaud, C., et al. 2000. Mol Biol Evol. 17: 1661-72. PMID: 11070054
  5. 表皮生长因子受体(EGFR)的 FUT8 重塑调控牛皮癣发病过程中表皮角质细胞的增殖  |  Kelel, M., et al. 2021. J Invest Dermatol. 141: 512-522. PMID: 32888953
  6. FUT8表达在各种恶性肿瘤的临床病理和患者生存中的作用:系统评价和荟萃分析。  |  Ma, M., et al. 2020. Aging (Albany NY). 13: 2212-2230. PMID: 33323540
  7. FUT8 介导的 B7H3 N-糖基化异常抑制了三阴性乳腺癌的免疫反应。  |  Huang, Y., et al. 2021. Nat Commun. 12: 2672. PMID: 33976130
  8. FUT8介导的核心富集促进肺动脉高压的肺血管重塑。  |  Zhang, W., et al. 2023. Aging Dis. 14: 1927-1944. PMID: 37196106
  9. 核心富集酶FUT8的独特结构域和活性调节。  |  Tomida, S., et al. 2024. Biochim Biophys Acta Gen Subj. 1868: 130561. PMID: 38218458
  10. FUT8在肿瘤发生中的多面作用:从通路到潜在临床应用。  |  Shi, M., et al. 2024. Int J Mol Sci. 25: PMID: 38256141
  11. FUT8介导的SEMA7A异常N-糖基化促进头颈部鳞状细胞癌的进展  |  Liu, Z., et al. 2024. Int J Oral Sci. 16: 26. PMID: 38548747
  12. 从人类胃癌 MKN45 细胞中纯化和克隆 GDP-L-Fuc:N-acetyl-beta-D-glucosaminide:alpha1-6 fucosyltransferase(alpha1-6 FucT)的 cDNA。  |  Yanagidani, S., et al. 1997. J Biochem. 121: 626-32. PMID: 9133635

订购信息

产品名称产品编号规格价格数量收藏夹

FucT-VIII 抗体 (B-10)

sc-271244
200 µg/ml
RMB2377.00

FucT-VIII (B-10): m-IgG Fc BP-HRP 套装

sc-529148
200 µg Ab; 10 µg BP
RMB2662.00

FucT-VIII (B-10): m-IgGκ BP-HRP 套装

sc-521890
200 µg Ab, 40 µg BP
RMB2662.00

FucT-VIII (B-10): m-IgG1 BP-HRP 套装

sc-543353
200 µg Ab; 20 µg BP
RMB2662.00

FucT-VIII 抗体 (B-10) AC

sc-271244 AC
500 µg/ml, 25% agarose
RMB3129.00

FucT-VIII 抗体 (B-10) HRP

sc-271244 HRP
200 µg/ml
RMB2377.00

FucT-VIII 抗体 (B-10) FITC

sc-271244 FITC
200 µg/ml
RMB2482.00

FucT-VIII 抗体 (B-10) PE

sc-271244 PE
200 µg/ml
RMB2580.00

FucT-VIII 抗体 (B-10) Alexa Fluor® 488

sc-271244 AF488
200 µg/ml
RMB2685.00

FucT-VIII 抗体 (B-10) Alexa Fluor® 546

sc-271244 AF546
200 µg/ml
RMB2685.00

FucT-VIII 抗体 (B-10) Alexa Fluor® 594

sc-271244 AF594
200 µg/ml
RMB2685.00

FucT-VIII 抗体 (B-10) Alexa Fluor® 647

sc-271244 AF647
200 µg/ml
RMB2685.00

FucT-VIII 抗体 (B-10) Alexa Fluor® 680

sc-271244 AF680
200 µg/ml
RMB2685.00

FucT-VIII 抗体 (B-10) Alexa Fluor® 790

sc-271244 AF790
200 µg/ml
RMB2685.00

I performed WB on total protein extracts from CRFK cells and revealed with the anti-FUT8 antibody at a 1:1500 dilution. I obtained bands at a weight of approximately 66 kDa (the expected weight) but I obtained two others (45 and 35 kDa respectively). What

Asked by: Itzha
Thank you for contacting Santa Cruz Biotechnology. For assistance please contact technical service. Please email [email protected] or call 800-457-3801.
Answered by: BlakeJ
Date published: 2025-03-27

I need to use an antibody to detect the protein in samples of bovine origin. Do you know if sc-271244: FucT-VIII (B-10) mouse monoclonal antibody will work?

Asked by: Professor Griffin
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2019-08-21
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Rated 4 out of 5 by from funcionó correctamenteLa muestra del anticuerpo funcionó correctamente en WB e inmunofluorescencia, detecta una sola banda en el peso de 65 kDa (correspondiente a fucT8 de gato). Se compró el anticuerpo pero nos enviaron un lote diferente al de la muestra, y este detecta múltiples bandas, el resultado no es limpio. Ya se modificaron las concentraciones de proteína y de anticuerpos, pero el resultado no es óptimo.
Date published: 2024-04-01
Rated 5 out of 5 by from Reliable antibody for WBThis antibody is well-cited for a variety of applications including WB, IP, IF, and ELISA. Strong, clear signal with little to no background. Great in human cell lines.
Date published: 2017-02-01
Rated 5 out of 5 by from Produced positive Western blot data of FucTProduced positive Western blot data of FucT-VIII expression in non-transfected and mouse FucT-VIII transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-10-14
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FucT-VIII 抗体 (B-10) is rated 4.7 out of 5 by 3.
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