Date published: 2025-9-8
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Fra1 Antibody (D-3) - Western Blotting - Image 353177
Fra-1 抗体 (D-3): sc-376148. MDA-MB-435S (A) 和 MDA-MB-231 (B) 全细胞裂解液及 NIH/3T3 核提取物 (C) 中 Fra-1 表达的 Western 印迹分析.
Fra-1 抗体 (D-3): sc-376148. MDA-MB-435S (A) 和 MDA-MB-231 (B) 全细胞裂解液及 NIH/3T3 核提取物 (C) 中 Fra-1 表达的 Western 印迹分析.

Fra1 抗体 (D-3): sc-376148

4.7(6)
寫評論提問

说明书
  • Fra1 抗体 D-3 是小鼠单克隆 IgG1 κ，Fra1抗体, 在23篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的Fra-1的N-terminus的氨基酸2-29之间
  • Fra1 抗体 (D-3) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Fra-1
  • 抗Fra1抗体（D-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-376148 X, 200 µg/0.1 ml)
  • 关于如何获取Fra1 (D-3): sc-376148的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 Fra1 抗体 (D-3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Fra1 抗体 (D-3) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Fra抗体，包括Fra-2

Fra-1抗体（D-3）是一种IgG1 κ鼠单克隆Fra-1抗体（也被指定为FRA1抗体或FOSL1抗体），它通过WB、IP、IF、IHC(P)和ELISA方法检测小鼠、大鼠和人类来源的Fra-1蛋白。Fra-1抗体（D-3）既可作为非偶联抗Fra-1抗体形式提供，也可作为多种偶联形式的抗Fra-1抗体提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。v-Fos原癌基因最初在两个独立的鼠骨肉瘤病毒分离物和一种禽肾母细胞瘤病毒中被检测到。c-Fos基因家族的成员，包括c-Fos、Fos B、Fra-1和Fra-2，编码核磷酸蛋白，这些核磷酸蛋白可被多种因子快速和短暂诱导，并作为几种基因的转录调节因子。与形成结合特定DNA反应元件的同源和异源二聚体的c-Jun蛋白不同，c-Fos蛋白仅在与Jun基因家族成员形成异源二聚体时才具有活性。此外，选定的ATF/CREB家族成员可以与Fos和Jun形成亮氨酸拉链二聚体。不同的二聚体对AP-1和CRE位点表现出不同的特异性和亲和力。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Fra1 抗体 (D-3)

sc-376148
200 µg/ml
RMB2377.00

Fra1 (D-3): m-IgG Fc BP-HRP 套装

sc-529826
200 µg Ab; 10 µg BP
RMB2662.00

Fra1 (D-3): m-IgGκ BP-HRP 套装

sc-522853
200 µg Ab, 40 µg BP
RMB2662.00

Fra1 (D-3): m-IgG1 BP-HRP 套装

sc-543709
200 µg Ab; 20 µg BP
RMB2662.00

Fra1 抗体 (D-3) X

sc-376148 X
200 µg/0.1 ml
RMB2377.00

Fra1 抗体 (D-3) AC

sc-376148 AC
500 µg/ml, 25% agarose
RMB3129.00

Fra1 抗体 (D-3) HRP

sc-376148 HRP
200 µg/ml
RMB2377.00

Fra1 抗体 (D-3) FITC

sc-376148 FITC
200 µg/ml
RMB2482.00

Fra1 抗体 (D-3) PE

sc-376148 PE
200 µg/ml
RMB2580.00

Fra1 抗体 (D-3) Alexa Fluor® 488

sc-376148 AF488
200 µg/ml
RMB2685.00

Fra1 抗体 (D-3) Alexa Fluor® 546

sc-376148 AF546
200 µg/ml
RMB2685.00

Fra1 抗体 (D-3) Alexa Fluor® 594

sc-376148 AF594
200 µg/ml
RMB2685.00

Fra1 抗体 (D-3) Alexa Fluor® 647

sc-376148 AF647
200 µg/ml
RMB2685.00

Fra1 抗体 (D-3) Alexa Fluor® 680

sc-376148 AF680
200 µg/ml
RMB2685.00

Fra1 抗体 (D-3) Alexa Fluor® 790

sc-376148 AF790
200 µg/ml
RMB2685.00

Fra1 (D-3) 阻断肽

sc-376148 P
100 µg/0.5 ml
RMB511.00

for western blot, what's the concentration did you use in the Hela cells shown in the datashe et? Thanks

Asked by: picia67
Thank you for your question. For Western Blotting experiments with this antibody we recommend a starting dilution of 1:100 and a dilution range of 1:100-1:1000.
Answered by: Tech Support Europe
Date published: 2021-02-18

Hello, I would like to use this antibody for ChIP. What dilution would you recommend? Do you have any validation data on the ChIP performance on this antibody?

Asked by: HesAmenya
Thank you for your question. This antibody has not yet been tested for ChIP, and we are not yet aware of any product citations using this particular antibody for any ChIP applications. We do extend our warranty to cover that research application for our TransCruz reagent for Gel Supershift and ChIP applications (sc-376148 X, 200 µg/0.1 ml). We currently offer a different antibody that has been published for use in ChIP applications. Please refer to our Fra-1 Antibody (C-12): sc-28310. And PMID's: 22198213 and 21088499.
Answered by: Technical Support
Date published: 2020-07-23

Welche Proteinmenge eignet sich am besten für einen Western Blot mit dem Fra-1 Antikörper?

Asked by: Neslihan
Vielen Dank für Ihre Frage. Die optimale Proteinmenge ist abhängig von dem verwendeten Sample. Generell empfehlen wir pro Bahn bei der Elektrophorese eine Proteinmenge von 40–60 µg bei Zelllysaten, 10–20 µg bei Kernextrakten, 5-10 µg bei transfizierten Zelllysaten, oder 10–20 ng bei aufgereinigtem Protein. What amount of protein is ideal for a western blot with Fra-1 Antibody (D-3): sc-376148? Thank you for your question. The ideal amount of protein is dependent on the sample that is being tested. We generally recommend 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-30

I would like to use Fra-1 Antibody (D-3): sc-376148 for double staining with another mouse monoclonal primary antibody. Is this possible?

Asked by: Trav11
Thank you for your question. In cases like this, we recommend using our directly conjugated mouse monoclonal primary antibodies. Currently, sc-376148 is available conjugated to HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Tech Service 8
Date published: 2017-01-31
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Rated 5 out of 5 by from good qualityreceived the samples weeks ago. blotting showed very strong signal
Date published: 2018-02-19
Rated 3 out of 5 by from Average featureswork well in WB at 1:500,Ges-1 Ccell,but when I recycle to use it,the titer is too inefficient,which led to a poor result.
Date published: 2017-12-05
Rated 5 out of 5 by from Works well for western blotThe antibody produced a good western blot for human tissue samples
Date published: 2016-12-19
Rated 5 out of 5 by from Positive detection of endogenously expressed FraPositive detection of endogenously expressed Fra-1 protein in NIH/3T3 cells (nuclear extracts used) through analysis by Western Blot. -SCBT QC
Date published: 2015-03-12
Rated 5 out of 5 by from Excellent immunoperoxidase nuclear and cytoplasmicExcellent immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human rectum tissue. -SCBT QC
Date published: 2015-03-08
Rated 5 out of 5 by from Good specificity shown by using transfectedGood specificity shown by using transfected and non-transfected 293T cells for over-expression of the human Fra-1 protein via Western Blot analysis. -SCBT QC
Date published: 2015-02-04
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Fra1 抗体 (D-3) is rated 4.7 out of 5 by 6.
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