Date published: 2025-9-7
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FR Antibody (E-11) - Western Blotting - Image 355234
FR 抗体 (E-11): sc-515521. 在 SHP-77 (A), A-431 (B), MH-S (C), KNRK (D) 和 PC-12 (E) 全细胞裂解液中 FR 表达的 Western 印迹分析.
FR 抗体 (E-11): sc-515521. 在 SHP-77 (A), A-431 (B), MH-S (C), KNRK (D) 和 PC-12 (E) 全细胞裂解液中 FR 表达的 Western 印迹分析.

FR 抗体 (E-11): sc-515521

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寫評論提問

说明书
  • FR抗体(E-11)是小鼠单克隆IgG2a κ, 在6篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的FR的C-terminus附近的氨基酸210-231之间
  • FR 抗体 (E-11) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测 α-FR, β-FR and γ-FR of human origin and α-FR, and β-FR of mouse and rat origin
  • 抗FR抗体（E-11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取FR (E-11): sc-515521的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 FR 抗体 (E-11) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与FR 抗体 (E-11) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 FR 抗体包括 和 β-FR

FR抗体（E-11）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）等方法检测小鼠、大鼠和人类样本中的叶酸受体（FR）。FR抗体（E-11）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物，可满足不同实验需求。叶酸受体家族在细胞摄取叶酸的过程中发挥着至关重要的作用，叶酸是DNA合成和修复以及氨基酸代谢所必需的一种重要维生素。该家族由五个成员组成：α、β、γ、γ'（由可变剪接产生）和δ，其中α-FR和β-FR在恶性组织中表达，因此具有特别重要的意义，成为癌症治疗的重要靶标。这些受体的独特结构包括α-FR和β-FR的糖基磷脂酰肌醇（GPI）锚，有助于它们定位到细胞膜，增强其介导5-甲基四氢叶酸内吞作用的能力。这种机制对于维持细胞内叶酸的平衡至关重要，尤其是在快速分裂的组织中，它强调了叶酸受体在正常生理和癌症生物学中的重要性。α-FR是一种高度选择性的肿瘤标志物，为靶向药物输送系统提供了潜力，该系统利用叶酸衍生物选择性地输送治疗剂到肿瘤细胞，从而提高治疗效果，同时最大限度地减少全身毒性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

FR 抗体 (E-11) 参考文献:

  1. 通过叶酸受体靶向给药。  |  Sudimack, J. and Lee, RJ. 2000. Adv Drug Deliv Rev. 41: 147-62. PMID: 10699311
  2. 叶酸受体 beta 型的分化依赖性视黄醇诱导，这是骨髓性白血病的潜在肿瘤靶标。  |  Wang, H., et al. 2000. Blood. 96: 3529-36. PMID: 11071651
  3. 肠道叶酸摄取的适应性调节：膳食叶酸缺乏的影响。  |  Said, HM., et al. 2000. Am J Physiol Cell Physiol. 279: C1889-95. PMID: 11078704
  4. 鉴定人类和小鼠的两个假定的新型叶酸受体基因  |  Spiegelstein, O., et al. 2000. Gene. 258: 117-25. PMID: 11111049
  5. 在叶酸耗竭的 CHL 细胞中，叶酸受体α（FR alpha）的合成速率受对介质叶酸水平敏感的翻译机制调节，而其 mRNA 的稳定过表达则由基因扩增和转录本半衰期的延长介导。  |  Zhu, WY., et al. 2001. J Cell Biochem. 81: 205-19. PMID: 11241661
  6. 靶向叶酸受体（FR）：ReI 共轭化合物在 FR 表达过高的癌细胞中的成像和细胞毒性。  |  Viola-Villegas, N., et al. 2008. ChemMedChem. 3: 1387-94. PMID: 18651631
  7. 使用对 pH 值敏感的 N-乙氧基苄基咪唑 (NEBI) 双功能交联剂开发叶酸受体 (FR) 靶向茚异喹啉。  |  Cao, Y. and Yang, J. 2014. Bioconjug Chem. 25: 873-8. PMID: 24758386
  8. 用于 FR 成像的 99m Tc 标记叶酸-PAMAM 树枝状聚合物的合成和初步评估。  |  Song, M., et al. 2017. Chem Biol Drug Des. 89: 755-761. PMID: 27910223
  9. 术后叶酸受体阳性循环肿瘤细胞（FR + CTCs）对肺浸润性腺癌（IAC）患者长期预后的临床意义。  |  Ma, Z., et al. 2024. Thorac Cancer. 15: 1060-1071. PMID: 38532562
  10. 由于缺乏糖基磷脂酰肌醇修饰的有效信号，γ 型叶酸受体主要是一种分泌蛋白：蛋白质特征和细胞类型特异性。  |  Shen, F., et al. 1995. Biochemistry. 34: 5660-5. PMID: 7727426
  11. 叶酸受体（FR-α）高亲和力同工型在人胎盘合胞滋养细胞和绒毛膜癌细胞中的选择性表达。  |  Prasad, PD., et al. 1994. Biochim Biophys Acta. 1223: 71-5. PMID: 8061055
  12. 编码γ/γ型叶酸受体的多态基因的结构和调控。  |  Wang, H., et al. 1998. Nucleic Acids Res. 26: 2132-42. PMID: 9547271

订购信息

产品名称产品编号规格价格数量收藏夹

FR 抗体 (E-11)

sc-515521
200 µg/ml
$316.00

FR (E-11): m-IgGκ BP-HRP 套装

sc-525082
200 µg Ab, 40 µg BP
$354.00

FR (E-11): m-IgG2a BP-HRP 套装

sc-546178
200 µg Ab; 10 µg BP
$354.00

FR 抗体 (E-11) AC

sc-515521 AC
500 µg/ml, 25% agarose
$416.00

FR 抗体 (E-11) HRP

sc-515521 HRP
200 µg/ml
$316.00

FR 抗体 (E-11) FITC

sc-515521 FITC
200 µg/ml
$330.00

FR 抗体 (E-11) PE

sc-515521 PE
200 µg/ml
$343.00

FR 抗体 (E-11) Alexa Fluor® 488

sc-515521 AF488
200 µg/ml
$357.00

FR 抗体 (E-11) Alexa Fluor® 546

sc-515521 AF546
200 µg/ml
$357.00

FR 抗体 (E-11) Alexa Fluor® 594

sc-515521 AF594
200 µg/ml
$357.00

FR 抗体 (E-11) Alexa Fluor® 647

sc-515521 AF647
200 µg/ml
$357.00

FR 抗体 (E-11) Alexa Fluor® 680

sc-515521 AF680
200 µg/ml
$357.00

FR 抗体 (E-11) Alexa Fluor® 790

sc-515521 AF790
200 µg/ml
$357.00

FR (E-11) 中和勝肽

sc-515521 P
100 µg/0.5 ml
$68.00

Bonjour, Je souhaiterai savoir si la référence FR (E-11) anticorps contre Folate receptor est suitable pour la cytométrie en flux ? Étant donné que les produits sont également couplés à des fluorochromes, je me pose la question. Merci.

Asked by: spotiron
Thank you for your question. FR Antibody (E-11) is only recommended for detection of α-FR, β-FR and γ-FR of human origin and α-FR, and β-FR of mouse and rat origin by WB, IP, IF, IHC(P) and ELISA. The antibody is also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM.
Answered by: Tech Support Europe
Date published: 2024-03-21

Can I use this product to detect murine macrophages (raw 264.7) FR using goat anti-mouse secondary antibody?

Asked by: Amir2
Thank you for your question. This FR antibody is recommended for detection in mouse samples. We have not specifically tested the antibody in RAW 264.7. This is a mouse monoclonal IgG2a, and so it can be detected using a secondary antibody that is goat anti-mouse IgG.
Answered by: Technical Support
Date published: 2020-03-19

hi,can you please tell me, FR antibody conjugated with FITC can be useful to find the folate receptors on colon cancer-based spheroids

Asked by: Lokesh
Thank you for your question. We have not tested this antibody (conjugated or unconjugated) for immunofluorescent staining of 3D spheroids.
Answered by: Technical Support
Date published: 2020-03-19

Hi, Can you please tell me what species your FR Antibody (E-11): sc-515521 recognizes? i.e. does it detect human and/or mouse FOLR1? Thanks! JSM

Asked by: JSKM
Thank you for your question. This antibody is reactive with mouse, rat and human.
Answered by: Technical Support
Date published: 2020-03-19

Hi, I have another question about your FR Antibody (E-11): sc-515521. Has it been evaluated for use in flow cytometry? Thanks!

Asked by: JSKM
The conjugated primary antibody is recommended and warrantied for flow cytometry (FCM), however we are still gathering data to show on our website. Check back for updates!
Answered by: Technical Support
Date published: 2020-12-16

Hi, We intend to procure FR (E-11): sc-515521. Can you please send us a free 10 ug to 20 ug sample? Please reach out to me in the following address. Thank you, Anubhab

Asked by: Anubhab
Thank you for your question. Please contact Technical Service for more information on how to receive a 10 μg sample of our well characterized monoclonal antibody.
Answered by: Technical Support
Date published: 2020-03-22

What application is the blocking peptide sc-515521 P appropriate for?

Asked by: Dr Ninau Qelp
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2020-03-22
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Rated 5 out of 5 by from KB细胞染色文献调研KB细胞高表达FR，试验结果也与预期一致，强阳。另外做了人的胎盘、肾脏的染色均为阳性，基本ok
Date published: 2020-06-12
Rated 5 out of 5 by from LE TOPEN A ACHETER UN ET TRES CONTENTE au plaisir de le ravoir
Date published: 2020-04-03
Rated 5 out of 5 by from good antibodyThis antibody have powerful band detected in a large number of cell lines, good specificity, the analysis results of Western blot were very strong and clear.We are in a very good place in that regard.
Date published: 2017-05-28
Rated 5 out of 5 by from Used to functionalize particlesI received a sample of this product and will be ordering a full vial. I used this antibody to functionalize particles in order to target certain cancer cells - particles bound to cells expressing FR and did not bind to non-FR-expressing cells. A great product!
Date published: 2017-05-16
Rated 5 out of 5 by from recommend use itWe used Western blot test mosue brain samples, dilution 1:200 effect is obvious, will continue to use this antibody.
Date published: 2017-02-03
Rated 5 out of 5 by from Western blot analysis of FR expression in SHP-77 (. Western blot analysis of FR expression in SHP-77 (A), A-431 (B), MH-S (C), KNRK (D) and PC-12 (E) whole cell lysates.
Date published: 2017-01-08
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FR 抗体 (E-11) is rated 5.0 out of 5 by 6.
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