Date published: 2025-9-7
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FOXE3 Antibody (D-6) - Western Blotting - Image 286021
FOXE3 抗体 (D-6): sc-377465. 非转染: sc-117752 (A) 和人 FOXE3 转染: sc-372347 (B) 293T 全细胞裂解液中 FOXE3 表达的 Western 印迹分析.
FOXE3 Antibody (D-6) - Western Blotting - Image 286021
FOXE3 抗体 (D-6): sc-377465. 非转染: sc-117752 (A) 和人 FOXE3 转染: sc-372347 (B) 293T 全细胞裂解液中 FOXE3 表达的 Western 印迹分析.
FOXE3 抗体 (D-6): sc-377465. 非转染: sc-117752 (A) 和人 FOXE3 转染: sc-372347 (B) 293T 全细胞裂解液中 FOXE3 表达的 Western 印迹分析.

FOXE3 抗体 (D-6): sc-377465

4.5(2)
寫評論提問

说明书
  • FOXE3抗体(D-6)是小鼠单克隆IgG1 κ, 在3篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列232-288位于mouse物种的FOXE3的C-terminus
  • 推荐用于 mouse, rat 和 human 来源的FOXE3 WB, IP, IF 和 ELISA检测
  • 可用于ChIP的TransCruz试剂(sc-377465 X, 200 µg/0.1 ml)
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是FOXE3 Antibody (D-6) for WB applications. 的首选辅助检测试剂，这些试剂现与FOXE3 Antibody (D-6) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外FOX抗体，包括FOXC1, FOXC2, FOXD1, FOXD3, FOXE3, FOXF2, FOXJ1, FOXJ2, FOXK1, FOXL1, FOXL2, FOXM1, FOXN1, FOXN2, FOXN4和FOXP1

人类叉头框（FOX）基因家族至少由43个成员组成，其中包括由288个氨基酸组成的FOXE3。FOXE3是一种翼状螺旋转录因子，在晶状体发育的最初阶段和晶状体囊闭合过程中起着至关重要的作用。FOXE3还可能作为促进生存和增殖的因素，同时防止晶状体上皮的分化。随着晶状体纤维的后部细胞开始分化，FOXE3的表达仅限于覆盖晶状体前表面的未分化细胞。先天性原发性无晶状体（CPA）是一种罕见的发育性疾病，由FOXE3基因的无效突变引起，以无晶状体为特征。CPA的发展通常在人类胚胎发育的第四或第五周受到刺激。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

FOXE3 抗体 (D-6)

sc-377465
200 µg/ml
$316.00

FOXE3 (D-6): m-IgG Fc BP-HRP 套装

sc-540723
200 µg Ab; 10 µg BP
$354.00

FOXE3 (D-6): m-IgG1 BP-HRP 套装

sc-542325
200 µg Ab; 20 µg BP
$354.00

FOXE3 抗体 (D-6) X

sc-377465 X
200 µg/0.1 ml
$316.00

Why does the blot show two different bands or could you precise what does each band correspond to ?

Asked by: Cangee
Thank you for your question. The western blot image shows cell lysates that were transfected with a truncated peptide corresponding to the antibody's binding site. Endogenous FOXE3 will produce a signal at 33 kDa in western blots.
Answered by: Tech Support Europe
Date published: 2022-03-31

Why does the blot show two bands, and neither of which is at 33 kDa (which is the predicted protein size for human FOXE3 protein)?

Asked by: SPadula
Thank you for your question. The Western blot data may not be representative of the endogenous molecular weight for FOXE3. This is detected in a human FOXE3 transfected 293T lysate. The observed molecular weight of FOXE3 may be higher than the predicted molecular weight as a result of post-translational modifications of the protein.
Answered by: Technical Support
Date published: 2022-03-31

I need to use an antibody to detect the protein in samples of avian origin. Do you know if sc-377465: FOXE3 (D-6) mouse monoclonal antibody will work?

Asked by: jenniferc
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2017-03-06
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Rated 4 out of 5 by from Hard to set, but works fineWe bought this product for a western blot on mouse lens epithelium. It took some time to find the right parameters to run the experiment, but when we found them, the antibody worked fine for our samples, and allowed quantification of FOXE3. The only problem remains the aspecific band at 50kDa, it would be interesting to understand what it corresponds to (obviously not FOXE3).
Date published: 2023-08-21
Rated 5 out of 5 by from Works well in WB in mouse FOXP1 transfectedWorks well in WB in mouse FOXP1 transfected 293T whole cell lysate. -SCBT QC
Date published: 2014-09-30
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FOXE3 抗体 (D-6) is rated 4.5 out of 5 by 2.
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