登陆账户

快速订购

购物车 (0)

FLIP<sub>L</sub> Antibody (5D8) - Western Blotting - Image 67359 — FLIPL 抗体 (5D8): sc-136160. 小鼠肾脏组织提取物 (A), MCF7 全细胞裂解液 (B) 和大鼠脾脏组织提取物 (C) 中 FLIPL 表达的 Western 印迹分析.

FLIPL Antibody (5D8) - Western Blotting - Image 67359 — FLIPL 抗体 (5D8): sc-136160. 小鼠肾脏组织提取物 (A), MCF7 全细胞裂解液 (B) 和大鼠脾脏组织提取物 (C) 中 FLIPL 表达的 Western 印迹分析.

FLIPL 抗体 (5D8): sc-136160. 小鼠肾脏组织提取物 (A), MCF7 全细胞裂解液 (B) 和大鼠脾脏组织提取物 (C) 中 FLIPL 表达的 Western 印迹分析.

FLIP_L 抗体 (5D8): sc-136160

Name: FLIPL抗体 (5D8)
Brand: SCBIO
SKU: sc-136160
Price: 2377.0 CNY
Availability: InStock

4.7(3)

寫評論提問

说明书

FLIPL 抗体 5D8 是小鼠单克隆 IgG₃，FLIP_L抗体, 在3篇文献中引用，规格为50 µg/0.5 ml
与重组蛋白结合，该重组蛋白与FLIP_L的1-376的氨基酸对应，来源于human
推荐用于 mouse, rat 和 human 来源的FLIP long isoform WB, IP 和 ELISA检测
看 FLIP_S/L (G-11): sc-5276，可查看有标记的 FLIP_L 抗体，包括AC, HRP, FITC, PE, Alexa Fluor^® 488, 594, 647, 680 和 790
目前，我们还没有完成FLIP_L 抗体 (5D8)的首选二抗检测试剂的鉴定。这项工作正在进行中。

查看产品引用文献(3)

快捷链接

FLIP_L 抗体 (5D8) 参考文献:

调节半胱天冬酶-8和FLICE抑制蛋白的表达是EB病毒在伯基特淋巴瘤中致癌的潜在机制。 | Tepper, CG. and Seldin, MF. 1999. Blood. 94: 1727-37. PMID: 10477698
Fas相关死亡结构域蛋白（FADD）和caspase-8通过FLICE抑制蛋白（FLIP）调控的途径介导Fas配体和肿瘤坏死因子相关凋亡诱导配体（TRAIL）对c-Fos的上调。 | Siegmund, D., et al. 2001. J Biol Chem. 276: 32585-90. PMID: 11384965
人卵巢癌细胞对肿瘤坏死因子α的抗性是核因子κB介导的Fas相关死亡结构域样白介素-1β转化酶样抑制蛋白诱导的结果。 | Xiao, CW., et al. 2003. Endocrinology. 144: 623-30. PMID: 12538625
TRAF2与细胞FLICE样抑制蛋白短链的结合可防止TNFR1介导的细胞凋亡。 | Kim, DJ., et al. 2008. J Mol Signal. 3: 2. PMID: 18190721
一氧化氮上调肺内皮细胞中caspase-8 mRNA的表达：JAK2/STAT-1信号的作用。 | Moujalled, DM., et al. 2012. Cell Death Differ. 19: 808-15. PMID: 22095280
病毒 FLICE 抑制蛋白（FLIPs）可阻止死亡受体诱导的细胞凋亡。 | Thome, M., et al. 1997. Nature. 386: 517-21. PMID: 9087414
Casper 是一种与 FADD 和 caspase 相关的细胞凋亡诱导剂。 | Shu, HB., et al. 1997. Immunity. 6: 751-63. PMID: 9208847
肿瘤坏死因子受体-1 和 CD-95 诱导细胞凋亡的新型抑制剂 I-FLICE。 | Hu, S., et al. 1997. J Biol Chem. 272: 17255-7. PMID: 9211860
细胞 FLIP 对死亡受体信号的抑制。 | Irmler, M., et al. 1997. Nature. 388: 190-5. PMID: 9217161
FLAME-1，一种新型 FADD 类抗凋亡分子，可调节 Fas/TNFR1 诱导的细胞凋亡。 | Srinivasula, SM., et al. 1997. J Biol Chem. 272: 18542-5. PMID: 9228018
MRIT是一种新型的含死亡效应结构域的蛋白质，它能与caspases和BclXL相互作用并引发细胞死亡。 | Han, DK., et al. 1997. Proc Natl Acad Sci U S A. 94: 11333-8. PMID: 9326610
CLARP是一种含死亡效应结构域的蛋白质，它与caspase-8相互作用并调节细胞凋亡。 | Inohara, N., et al. 1997. Proc Natl Acad Sci U S A. 94: 10717-22. PMID: 9380701

订购信息

产品名称	产品编号	规格	价格	数量		收藏夹
FLIP_L 抗体 (5D8)	sc-136160	50 µg/0.5 ml	RMB2377.00

This antibody is described as anti-FLIP-L, with a companion product that is described as anti-FLIP S/L. But the epitope used is amino acids 1-376 of human FLIP-L. This epitope will also encompass FLIP-S. Won't this antibody also recognize FLIP-S?

Asked by: LisaCT

Thank you for your question. While (5D8) is raised against a recombinant protein corresponding to amino acids 1-376 of 55 kDa long form (c-FLIPL) of human origin, and a portion of this region does encompass 24-26 kDa short form (c-FLIPS), current observations in western blotting (5D8): sc-136160 on mouse kidney tissue extract, MCF7 whole cell lysate, and rat spleen tissue extract, indicate detection of only the 55kDa long form of c-FLIP. Please refer to the following article link for proteolytic mapping of post-translational c-FLIP. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4219646/

Answered by: BlakeJ

Date published: 2022-11-17

For Western Blot, is it recommended to use denatured or non-denatured conditions with FLIPL (5D8): sc-136160 antibody?

Asked by: TinTin

Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.

Answered by: Technical Support

Date published: 2017-03-27

Rated 5 out of 5 by SCBT QC Lab from Good for Western BlotThis antibody has been used for western blotting in published research. -SCBT QC

Date published: 2023-09-14

Rated 5 out of 5 by Yixiao Feng from Suitable for WBWorked well in Western blot. Not sure about other expts.

Date published: 2017-03-29

Rated 4 out of 5 by SS from Worked really well in multiple whole cellWorked really well in multiple whole cell lysates such as mouse kidney tissue extract, MCF7 whole cell lysate, and rat spleen tissue extract. -SCBT QC

Date published: 2013-06-26

FLIP L 抗体 (5D8) is rated 4.7 out of 5 by 3.

Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。

种属	基因名称	基因ID	染色体位置	亚型（mRNA）登录号	蛋白质登录号	OMIM™ 号码
Human	CFLAR	Entrez Gene ID: 8837	2q33.1	XM_017005189, XM_017005190, XM_017005191, XM_017005192, XM_017005193, XM_017005194, XM_017005195, XM_017005196, XM_017005197, XM_017005198, NM_001127183, NM_001127184, NM_001202515, NM_001202516, NM_001202517, NM_001202518, NM_001202519, NM_001308042, NM_001308043, NM_003879	UniProt ID: O15519	603599
Mouse	Cflar	Entrez Gene ID: 12633	1 C1.3	XM_006495635, XM_011238420, XM_011238421, XM_011238422, XM_011238423, XM_017313559, XM_017313595, NM_001289704, NM_001293804, NM_001293805, NM_009805, NM_207653	UniProt ID: O35732	N/A

FLIPL 抗体 (5D8): sc-136160

FLIPL 抗体 (5D8) 参考文献:

優選的 FLIPS/L 抗體是 FLIPS/L 抗體 (G-11): sc-5276.

FLIPL 抗体 (5D8)

This antibody is described as anti-FLIP-L, with a companion product that is described as anti-FLIP S/L. But the epitope used is amino acids 1-376 of human FLIP-L. This epitope will also encompass FLIP-S. Won't this antibody also recognize FLIP-S?

For Western Blot, is it recommended to use denatured or non-denatured conditions with FLIPL (5D8): sc-136160 antibody?

FLIP_L 抗体 (5D8): sc-136160

FLIP_L 抗体 (5D8) 参考文献:

優選的 FLIP_S/L 抗體是 FLIP_S/L 抗體 (G-11): sc-5276.

FLIP_L 抗体 (5D8)