Date published: 2025-9-7
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FKHRL1/FOXO3a Antibody (D-12) - Western Blotting - Image 380417
FKHRL1 抗体 (D-12): sc-48348. MDA-MB-231 (A), A-673 (B), RD (C), SJRH30 (D) 和 TF-1 (E) 全细胞裂解液中 FKHRL1 表达的 Western 印迹分析.
FKHRL1 抗体 (D-12): sc-48348. MDA-MB-231 (A), A-673 (B), RD (C), SJRH30 (D) 和 TF-1 (E) 全细胞裂解液中 FKHRL1 表达的 Western 印迹分析.

FKHRL1/FOXO3a 抗体 (D-12): sc-48348

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寫評論提問

说明书
  • FKHRL1/FOXO3a 抗体 D-12 是小鼠单克隆 IgG1 κ，FKHRL1/FOXO3a抗体, 在30篇文献中引用，规格为200 µg/ml
  • 免疫human物种的FKHRL1的氨基酸329-472
  • FKHRL1/FOXO3a 抗体 (D-12) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 FKHRL1
  • 抗FKHRL1/FOXO3a抗体（D-12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-48348 X, 200 µg/0.1 ml)
  • 关于如何获取FKHRL1/FOXO3a (D-12): sc-48348的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是FKHRL1/FOXO3a Antibody (D-12) for WB applications. 的首选辅助检测试剂，这些试剂现与FKHRL1/FOXO3a Antibody (D-12) 打包提供（请参阅下面的订购信息）。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

FKHR抗体

FKHRL1抗体（D-12）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的FKHRL1蛋白。FKHRL1 (D-12) 抗体有非结合型和多种结合型可供选择，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。FKHRL1蛋白，也称为FOXO3或FOXO3A，是一种关键的转录激活因子，由673个氨基酸组成，在调节细胞凋亡和细胞周期进展等细胞过程中发挥着重要作用。FOXO3的调节是通过翻译后修饰，特别是丝氨酸/苏氨酸激酶Akt1的磷酸化来实现的。当丝氨酸32和丝氨酸253被磷酸化时，FOXO3通过与14-3-3蛋白结合而被隔离在细胞质中，从而抑制转录活性。在生长因子缺失的条件下，FOXO3经历去磷酸化，转移到细胞核中，激活促凋亡基因的表达。这种动态调节对于维持细胞稳态和应激信号至关重要，使FOXO3成为癌症和代谢性疾病研究的重要靶标。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

FKHRL1/FOXO3a 抗体 (D-12) 参考文献:

  1. Akt 通过磷酸化和抑制叉头转录因子来促进细胞存活。  |  Brunet, A., et al. 1999. Cell. 96: 857-68. PMID: 10102273
  2. 蛋白激酶 SGK 通过磷酸化叉头转录因子 FKHRL1（FOXO3a）来介导生存信号。  |  Brunet, A., et al. 2001. Mol Cell Biol. 21: 952-65. PMID: 11154281
  3. 保守的 MST-FOXO 信号通路介导氧化应激反应并延长寿命。  |  Lehtinen, MK., et al. 2006. Cell. 125: 987-1001. PMID: 16751106
  4. FKHRL1介导的Noxa和Bim表达通过神经母细胞瘤细胞中的线粒体诱导细胞凋亡。  |  Obexer, P., et al. 2007. Cell Death Differ. 14: 534-47. PMID: 16888645
  5. 活化能蛋白激酶直接调节哺乳动物的 FOXO3 转录因子。  |  Greer, EL., et al. 2007. J Biol Chem. 282: 30107-19. PMID: 17711846
  6. 人类 FOXO3a-DBD/DNA 复合物的晶体结构表明了翻译后修饰的影响。  |  Tsai, KL., et al. 2007. Nucleic Acids Res. 35: 6984-94. PMID: 17940099
  7. 叉头转录因子 FOXO3 通过其结合伙伴 DNA 复制因子 Cdt1 调节细胞周期的进展。  |  Zhang, Y., et al. 2012. Proc Natl Acad Sci U S A. 109: 5717-22. PMID: 22451935
  8. 腺病毒介导的FKHRL1/TM使黑色素瘤细胞对替莫唑胺诱导的细胞凋亡敏感。  |  Egger, M., et al. 2014. Hum Gene Ther Clin Dev.. PMID: 25141261
  9. 腺病毒介导的FKHRL1/TM使黑色素瘤细胞对替莫唑胺诱导的细胞凋亡敏感。  |  Egger, ME., et al. 2014. Hum Gene Ther Clin Dev. 25: 186-95. PMID: 25238278
  10. 通过FKHRL1/FOXO3a激活SIRT6可抑制胶质母细胞瘤细胞的Warburg效应。  |  Dong, Z., et al. 2019. Cell Signal. 60: 100-113. PMID: 31004738
  11. AF6q21 是 t(6;11)(q21;q23)中 MLL 基因的一个新伙伴，它定义了一个叉头转录因子亚家族。  |  Hillion, J., et al. 1997. Blood. 90: 3714-9. PMID: 9345057
  12. 组成 FKHR 类基因亚家族的三个人类叉头基因的克隆和特征描述。  |  Anderson, MJ., et al. 1998. Genomics. 47: 187-99. PMID: 9479491

订购信息

产品名称产品编号规格价格数量收藏夹

FKHRL1/FOXO3a 抗体 (D-12)

sc-48348
200 µg/ml
RMB2377.00

FKHRL1/FOXO3a (D-12): m-IgG Fc BP-HRP 套装

sc-526742
200 µg Ab; 10 µg BP
RMB2662.00

FKHRL1/FOXO3a (D-12): m-IgG1 BP-HRP 套装

sc-532115
200 µg Ab; 20 µg BP
RMB2662.00

FKHRL1/FOXO3a 抗体 (D-12) X

sc-48348 X
200 µg/0.1 ml
RMB2377.00

FKHRL1/FOXO3a 抗体 (D-12) AC

sc-48348 AC
500 µg/ml, 25% agarose
RMB3129.00

FKHRL1/FOXO3a 抗体 (D-12) HRP

sc-48348 HRP
200 µg/ml
RMB2377.00

FKHRL1/FOXO3a 抗体 (D-12) FITC

sc-48348 FITC
200 µg/ml
RMB2482.00

FKHRL1/FOXO3a 抗体 (D-12) PE

sc-48348 PE
200 µg/ml
RMB2580.00

FKHRL1/FOXO3a 抗体 (D-12) Alexa Fluor® 488

sc-48348 AF488
200 µg/ml
RMB2685.00

FKHRL1/FOXO3a 抗体 (D-12) Alexa Fluor® 546

sc-48348 AF546
200 µg/ml
RMB2685.00

FKHRL1/FOXO3a 抗体 (D-12) Alexa Fluor® 594

sc-48348 AF594
200 µg/ml
RMB2685.00

FKHRL1/FOXO3a 抗体 (D-12) Alexa Fluor® 647

sc-48348 AF647
200 µg/ml
RMB2685.00

FKHRL1/FOXO3a 抗体 (D-12) Alexa Fluor® 680

sc-48348 AF680
200 µg/ml
RMB2685.00

FKHRL1/FOXO3a 抗体 (D-12) Alexa Fluor® 790

sc-48348 AF790
200 µg/ml
RMB2685.00

Can this antibody act as IF in rats

Asked by: renxue
Thank you for your question. Anti-FKHRL1/FOXO3a Antibody (D-12) is recommended for detection of FKHRL1 of mouse, rat and human origin by WB, IP, IF and ELISA, so it would be suitable for your proposed experiment.
Answered by: Tech Support Europe
Date published: 2022-03-25

which dilution i should use for Chip assay .

Asked by: rania
Thank you for your question. This antibody has not yet been tested of published for use in ChIP applications. We do warranty our TransCruz reagent (sc-48348 X) for use in ChIP applications. We recommend using 0.1-1 µl TransCruz reagent (0.2–2 µg) for 100–500 µg supernatant.
Answered by: Technical Support
Date published: 2019-07-13

Hello I would like to request free sample of this antibody please

Asked by: MMARTINEZ
Thank you for your question. Please contact Technical Service for more information on how to receive a 10 μg sample of our well characterized monoclonal antibody.
Answered by: Technical Support
Date published: 2018-12-05

When should the TransCruz reagent sc-48348 X be used instead of the standard form sc-48348?

Asked by: Germaine
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from Segnale buono in WBHo confrontato questo anticorpo con un'alternativa e il segnale era buono seppure leggermente più debole (WB).
Date published: 2021-05-20
Rated 3 out of 5 by from 功效不是很好，没有官网图片那么清晰。抗体按照1:300浓度，4℃孵育24h，与内参进行比较，效果不是很好。并且曝光时间很长，并不是很好用。
Date published: 2018-01-13
Rated 5 out of 5 by from verry wellthe antibody works well.The expression of it in human is strong signal.
Date published: 2017-12-14
Rated 5 out of 5 by from It works well!The antibidy is very good for western blot and IF. It works very well.
Date published: 2017-12-07
Rated 4 out of 5 by from Good antibodyI use this antibody for IF application in human melanocytes. Results were great and pictures showed clear results.
Date published: 2017-11-16
Rated 5 out of 5 by from Excellent antibodyI bought this antibody a month ago. It works very well for WB, IP and CHIP assays.
Date published: 2017-11-13
Rated 4 out of 5 by from can be used in WBI use it in WB . SK-N-SH cell lysis used。 10% gel，electrophoresis： 30 mA,90 min。 transfer membrane ：300 ma，45min。5% milk block，1：:75 first antibody overnight。1X TTBS wash 3 times ，second antibody 1:2000 ，1 h ， temperature。then 1X TTBS wash 3 times。 2 min can appearance band，which is a specific band ,which is weak.
Date published: 2017-09-20
Rated 4 out of 5 by from goodI bought this antibody for Western blot experiments. The results presented a band without non-specific background! It's a good antibody for WB experiment!
Date published: 2017-08-02
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FKHRL1/FOXO3a 抗体 (D-12) is rated 4.4 out of 5 by 17.
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