Date published: 2026-10-24

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Fibronectin Antibody (2755-8) - Western Blotting - Image 378991
纤连蛋白抗体 (2755-8): sc-69681. U-87 MG (A) 和 ACHN (B) 全细胞裂解液中纤维连接蛋白表达的 Western 印迹分析.
纤连蛋白抗体 (2755-8): sc-69681. U-87 MG (A) 和 ACHN (B) 全细胞裂解液中纤维连接蛋白表达的 Western 印迹分析.

Fibronectin 抗体 (2755-8): sc-69681

4.3(4)
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说明书
  • Fibronectin抗体(2755-8)是小鼠单克隆IgG1 κ, 在26篇文献中引用，规格为200 µg/ml
  • 针对human的T细胞白血病活检
  • 推荐用于 mouse, rat 和 human 来源的Fibronectin WB, IP, IF 和 IHC(P)检测
  • 看 Fibronectin (EP5): sc-8422，可查看有标记的 Fibronectin 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRP和 m-IgG1 BP-HRP是Fibronectin Antibody (2755-8) for WB and IHC(P) applications. 的首选辅助检测试剂，这些试剂现与Fibronectin Antibody (2755-8) 打包提供（请参阅下面的订购信息）。
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纤维连接蛋白是一种细胞外基质糖蛋白，存在于大多数细胞表面、细胞外液和血浆中。它是一种高分子量的异二聚体蛋白，最初被发现是病毒转化细胞表面缺失的蛋白质，已被证明参与各种功能，包括细胞粘附、细胞运动和伤口愈合。选择性剪接和糖基化产生了几种不同形式的纤维连接蛋白，其中一些表现出有限的组织分布或与恶性肿瘤有关。已经证明，肌成纤维细胞表型的形成与迪皮特伦病中糖基化纤维连接蛋白和纤维连接蛋白剪接变异体的发生有关。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Fibronectin 抗体 (2755-8) 参考文献:

  1. 人血浆纤维粘连蛋白的统计构象。  |  Pelta, J., et al. 2000. Biochemistry. 39: 5146-54. PMID: 10819982
  2. 抗坏血酸钠抑制细胞增殖和纤维蛋白的生物合成。  |  Péterszegi, G., et al. 2002. Eur J Clin Invest. 32: 372-80. PMID: 12064290
  3. 从用作伤口愈合剂的人类胎盘提取物中分离出类似纤维粘连蛋白 III 型的多肽。  |  Chakraborty, PD. and Bhattacharyya, D. 2005. J Chromatogr B Analyt Technol Biomed Life Sci. 818: 67-73. PMID: 15722046
  4. 在没有完整纤连蛋白的情况下，纤连蛋白 N 端 70 kDa 片段与细胞表面纤连蛋白组装位点结合。  |  Tomasini-Johansson, BR., et al. 2006. Matrix Biol. 25: 282-93. PMID: 16567085
  5. 将纤维连接蛋白组装成细胞外基质。  |  Mosher, DF., et al. 1991. Ann N Y Acad Sci. 614: 167-80. PMID: 1673833
  6. 外源性或内源性纤维粘连蛋白都能促进人类内皮细胞的粘附。  |  Clark, RA., et al. 1986. J Cell Sci. 82: 263-80. PMID: 3539950
  7. 纤维粘连蛋白在成纤维细胞迁移到血浆凝块中的作用  |  Knox, P., et al. 1986. J Cell Biol. 102: 2318-23. PMID: 3711148
  8. 正常人类妊娠期间胎盘中纤维粘连蛋白分布的变化。  |  Yamaguchi, Y., et al. 1985. Am J Obstet Gynecol. 152: 715-8. PMID: 3895942
  9. 纤连蛋白由人类巨噬细胞产生。  |  Alitalo, K., et al. 1980. J Exp Med. 151: 602-13. PMID: 7359083
  10. 纤维粘连蛋白与 I 型胶原蛋白和 IV 型胶原蛋白相互作用的差异。  |  Shimizu, M., et al. 1997. Biochim Biophys Acta. 1339: 53-61. PMID: 9165099

订购信息

产品名称产品编号规格价格数量收藏夹

Fibronectin 抗体 (2755-8)

sc-69681
200 µg/ml
CNY2422.00

Fibronectin (2755-8): m-IgG Fc BP-HRP 套装

sc-539216
200 µg Ab; 10 µg BP
CNY2715.00

Fibronectin (2755-8): m-IgG1 BP-HRP 套装

sc-541332
200 µg Ab; 20 µg BP
CNY2715.00

请问该Fibronectin抗体是否能在普通WB条件(还原性，变性)下进行实验？

Asked by: Anonymous
Thank you for your question. This antibody has been tested for denaturing reducing conditions in house, this would be suitable for your application. 感谢您的问题。我们实验室通过WB检测过还原变性条件。因此这支抗体是满足您的实验要求的。
Answered by: Technical Support 9
Date published: 2019-09-02

I am using Fibronectin (2755-8): sc-69681 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: DefinitelyNotMatt
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by Chaoqun from IF for paraffin embedded mouse lungI used this antibody at 1:50 for staining the paraffin embedded mouse lung, and it worked very well.
Date published: 2017-05-14
Rated 5 out of 5 by sticky from specifically stains fibronectinThis antibody specifically stains fibronectin, which is an extra cellular matrix protein. As a result, the cell boundary of cardiomyocytes was clearly stained.
Date published: 2017-01-21
Rated 2 out of 5 by AB from Clean blots in WesternClean blots in Western -SCBT QC
Date published: 2014-10-29
Rated 5 out of 5 by QZ from Human HFFs stained by IHCHuman HFFs stained by IHC, good localization. -SCBT Publication Review
Date published: 2012-10-02
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Fibronectin 抗体 (2755-8) is rated 4.3 out of 5 by 4.
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