Date published: 2026-11-7

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Fibroblast Marker Antibody (ER-TR7) - Western Blotting - Image 370761
成纤维细胞标记物抗体 (ER-TR7) HRP: sc-73355 HRP. 直接 Western 印迹分析 3611-RF 全细胞裂解液中成纤维细胞标记物的表达.
Fibroblast Marker Antibody (ER-TR7) - Western Blotting - Image 370761
成纤维细胞标记物抗体 (ER-TR7) HRP: sc-73355 HRP. 直接 Western 印迹分析 3611-RF 全细胞裂解液中成纤维细胞标记物的表达.
成纤维细胞标记物抗体 (ER-TR7) HRP: sc-73355 HRP. 直接 Western 印迹分析 3611-RF 全细胞裂解液中成纤维细胞标记物的表达.

Fibroblast Marker 抗体 (ER-TR7): sc-73355

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寫評論提問
说明书
  • Fibroblast Marker 抗体 ER-TR7 是大鼠单克隆 IgG2a，Fibroblast Marker抗体, 在105篇文献中引用，规格为200 µg/ml
  • 针对mouse来源的胸腺网状细胞
  • Fibroblast Marker 抗体 (ER-TR7) 推荐用于 WB, IP, IF 和 FCM，检测mouse, rat 和human 来源的 reticular fibroblasts; 不会与 purified Laminin, Collagen Types I-V, Fibronectin or Nidogen产生交联反应
  • 抗Fibroblast Marker抗体（ER-TR7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 目前，我们还没有完成Fibroblast Marker 抗体 (ER-TR7)的首选二抗检测试剂的鉴定。这项工作正在进行中。
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研究信息

関連項目

额外Fibroblast抗体，包括Human Thymic Fibroblasts

成纤维细胞标记物抗体（ER-TR7）是一种鼠单克隆IgG2a抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和流式细胞术（FCM）检测小鼠、大鼠和人类样本中的成纤维细胞标记物。成纤维细胞标记物（ER-TR7）抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。成纤维细胞在合成和维持细胞外基质方面发挥着关键作用，而细胞外基质对于组织的完整性和修复至关重要。这些细胞可产生胶原蛋白、糖胺聚糖和糖蛋白等关键成分，为伤口愈合提供结构支持。成纤维细胞的动态特性使其能够适应各种组织环境，而其增殖能力使其成为生物研究中的宝贵模型。此外，中间丝蛋白（如Vimentin）的表达可作为识别成纤维细胞和了解其中胚层起源的标记。成纤维细胞标记物（ER-TR7）单克隆抗体推动了成纤维细胞生物学研究的发展，使科学家能够探索细胞外基质中的各种功能和相互作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

含汞：所有HRP偶联物（包括组合装）均含有硫柳汞，这是一种汞化合物。请勿丢弃在垃圾中。应按照当地、州和联邦法规，将其作为危险化学品废弃物进行处置。

下载硫柳汞安全数据表（SDS）

Fibroblast Marker 抗体 (ER-TR7) 参考文献:

  1. 全长人成纤维细胞活化蛋白的表达, 纯化和动力学特征。  |  Sun, S., et al. 2002. Protein Expr Purif. 24: 274-81. PMID: 11858723
  2. 人成纤维细胞在三维胶原凝胶中对非侵入性电刺激的迁移。I. 诱导三维细胞运动的特征。  |  Sun, S., et al. 2004. Tissue Eng. 10: 1548-57. PMID: 15588414
  3. 多壁纳米碳管和纳米离子对人类皮肤成纤维细胞的细胞毒性机制的分子表征。  |  Ding, L., et al. 2005. Nano Lett. 5: 2448-64. PMID: 16351195
  4. 转基因大豆种子中碱性成纤维细胞生长因子的高水平表达及其生物活性鉴定。  |  Ding, SH., et al. 2006. Biotechnol Lett. 28: 869-75. PMID: 16786271
  5. 在羟基离子植入的 PHB 薄膜表面培养的成纤维细胞的形态。  |  Hou, T., et al. 2006. J Biomater Sci Polym Ed. 17: 735-46. PMID: 16909942
  6. 利用垂直扫描干涉仪表征生物活性表面上的成纤维细胞形态。  |  Revell, CM., et al. 2006. Matrix Biol. 25: 523-33. PMID: 16962756
  7. 普洱茶提取物的自由基清除效应及其对人成纤维细胞氧化损伤的保护作用  |  Jie, G., et al. 2006. J Agric Food Chem. 54: 8058-64. PMID: 17032009
  8. 成纤维细胞标记物的鉴定和特征描述：FSP1.  |  Strutz, F., et al. 1995. J Cell Biol. 130: 393-405. PMID: 7615639
  9. 肌球蛋白 II 活性对成纤维细胞硬度的影响  |  Schäfer, A. and Radmacher, M. 2005. Acta Biomater. 1: 273-80. PMID: 16701806

订购信息

产品名称产品编号规格价格数量收藏夹

Fibroblast Marker 抗体 (ER-TR7)

sc-73355
200 µg/ml
CNY2422.00

Fibroblast Marker 抗体 (ER-TR7) AC

sc-73355 AC
500 µg/ml, 25% agarose
CNY3189.00

Fibroblast Marker 抗体 (ER-TR7) HRP

sc-73355 HRP
200 µg/ml
CNY2422.00

Fibroblast Marker 抗体 (ER-TR7) FITC

sc-73355 FITC
200 µg/ml
CNY2527.00

Fibroblast Marker 抗体 (ER-TR7) PE

sc-73355 PE
200 µg/ml
CNY2625.00

Fibroblast Marker 抗体 (ER-TR7) Alexa Fluor® 488

sc-73355 AF488
200 µg/ml
CNY2738.00

Fibroblast Marker 抗体 (ER-TR7) Alexa Fluor® 546

sc-73355 AF546
200 µg/ml
CNY2738.00

Fibroblast Marker 抗体 (ER-TR7) Alexa Fluor® 594

sc-73355 AF594
200 µg/ml
CNY2738.00

Fibroblast Marker 抗体 (ER-TR7) Alexa Fluor® 647

sc-73355 AF647
200 µg/ml
CNY2738.00

Fibroblast Marker 抗体 (ER-TR7) Alexa Fluor® 680

sc-73355 AF680
200 µg/ml
CNY2738.00

Fibroblast Marker 抗体 (ER-TR7) Alexa Fluor® 790

sc-73355 AF790
200 µg/ml
CNY2738.00

Is it known whether the monoclonal antibody reacts with chicken fibroblasts?

Asked by: Harrie
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-05-18

I want to use this antibody to verify the identity of fibroblasts using Flow. Which fluorescent label version do you suggest?

Asked by: EdLe
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

Hi can I ask for a sample.

Asked by: linguo
Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Technical Support
Date published: 2020-02-19

Can I use this antibody for sorting of fibroblasts from mesenchymal stem cells ?

Asked by: gbjeong
Thank you for your question. This antibody is recommended for Flow cytometry, so sorting using a flow cytometer is fine. It has not been tested for any other sorting method.
Answered by: Tech Service
Date published: 2020-01-14

Is this the same clone as the Abcam antibody- clone: ab51824?

Asked by: clg50
Thank you for your question. Yes, this rat monoclonal antibody, clone designation ER-TR7 is commercially available from different suppliers.
Answered by: Technical Support
Date published: 2019-01-26

Has this antibody been tested for cross-reactivity?

Asked by: AbPolly
Thank you for your inquiry. Our Fibroblast Marker (ER-TR7) sc-73355 is known not to cross react with laminin, collagen types I-V, fibronectin or nidogen. Please contact our Technical Services Department for details or further questions about reactivity.
Answered by: Technical Support 12
Date published: 2017-03-29
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Rated 5 out of 5 by sophia2023 from 可以用于IF，能染出正确结构我们实验室曾经购买过这支抗体，能够正确染出成纤维细胞的结构。推荐IF使用1:100或者1:50的染色比例，效果会更理想。
Date published: 2023-08-30
Rated 5 out of 5 by Michelle B from Alexa Fluor 594 ReviewAnti-Fibroblast Marker Antibody (ER-TR7) Alexa Fluor 594 arrived quickly and a works well!
Date published: 2022-08-27
Rated 5 out of 5 by amn2720 from Good antibody to label fibroblastWorks and label both meningeal and perivascualr fibroblast in CNS.
Date published: 2022-06-20
Rated 4 out of 5 by kawaNIH from Nice work for immunofluorescentI used this antibody with the following conditions and nice worked . sample: cryosection of prefixed neonatal mouse heart blocking: 10% normal donkey serum antibody condition: 1:50, 4C overnight 2nd antibody: anti-Rat Alexa 633
Date published: 2017-09-01
Rated 1 out of 5 by Janetweber from Poor result by IF on fibroblast cell graftCultured fibroblasts were transplanted into monkey spinal cord. Graft seemed to survive but we saw no convincing signal using this antibody at 1:100. Tissue was paraformaldehyde fixed and processed free floating with Alexa Fluor secondary antibody.
Date published: 2017-06-15
Rated 5 out of 5 by jenni from Well publishedFibroblast Marker Antibody (ER-TR7) has been well cited in multiple publications
Date published: 2017-01-17
Rated 4 out of 5 by Yu from Used this in IF in mouse cells and got correctUsed this in IF in mouse cells and got correct staining.
Date published: 2015-09-14
Rated 5 out of 5 by D__Y_ from Worked well in WBWorked well in WB
Date published: 2015-09-04
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Fibroblast Marker抗体 (ER-TR7) is rated 4.4 out of 5 by 10.
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